白细胞介素-10与牙周炎

白细胞介素-10(IL-10)是一种抗炎细胞因子,在牙周病的发生发展过程中,尤其在复杂的细胞因子网络调节中发挥着重要作用。本文就IL-10与牙周炎的关系作一综述。     

IL-10mRNA及其蛋白在慢性牙周炎牙龈组织中的表达

目的检测IL-10 mRNA及其蛋白在慢性牙周炎牙龈组织中的表达及其组织细胞来源.方法随机选择12例慢性牙周炎翻瓣术患者作为牙周炎组,10例龈切术患者作为牙龈炎组,6例阻生牙拔除术患者作为健康对照组.分别采用原位杂交和免疫组化检测技术,检测各组牙龈标本中IL-10的表达.每组IL-10 mRNA及蛋白两种水平间的比较采用秩和检验;各组间数据的两两比较采用单因素方差分析.结果IL-10 mRNA及其蛋白在牙周局部牙龈组织均有表达,表达细胞类型有淋巴细胞、浆细胞、巨噬细胞及成纤维细胞等.IL-10在mRNA水平及蛋白水平表达无显著差异(P＞0.05)(牙龈炎组P＜0.05).牙周炎组IL-10表达强度显著高于健康对照组和牙龈炎组(P＜0.01),牙周炎组IL-10 mRNA表达强度显著高于健康对照组(P＜0.01),但与牙龈炎组比较差异无显著性(P＞0.05).结论IL-10在牙周组织中存在局部分泌机制.     

白细胞介素-10与牙周炎

白细胞介素-10(IL-10)是一种抗炎细胞因子，在牙周病的发生发展过程中，尤其在复杂的细胞因子网络调节中发挥着重要作用。本文就IL-10与牙周炎的关系作一综述。     

白细胞介素-10基因型与墨玉县维吾尔族成人慢性牙周炎易患性的相关性研究

目的 探讨白细胞介素-10（IL-10）基因-597（C/A）位点单核苷酸多态性与新疆维吾尔自治区墨玉县维吾尔族成人慢性牙周炎（CP）之间的相关性。方法 随机选取2013年4—5月本课题组对新疆墨玉县维吾尔族成人进行的口腔健康流行病学调查资料及采集的颊黏膜拭子样本共300例,根据纳入排除标准将其分为健康对照组、轻度CP组和中重度CP组,每组各100例。采用四引物等位基因特异性聚合酶链反应扩增法检测受试者IL-10-597位点基因型和等位基因的分布。采用卡方检验、有序多分类Logistic回归等方法进行统计学分析。结果 IL-10-597基因型及等位基因频率在健康对照组、轻度CP、中重度CP组分布的差异均无统计学意义（P>0.05）;抽取样本人群的年龄与CP具有相关性,55~65岁人群患CP的风险是低于35岁人群的25倍（OR=25.56,P<0.001）。结论 IL-10-597（C/A）位点单核苷酸多态性与维吾尔族成人CP的发病风险无明显相关性。     

IL-1β与IL-10mRNA在牙龈组织中的表达

目的:检测IL-1β与IL-10mRNA在正常牙龈及牙周炎患者牙龈组织中的表达,探讨二者与炎症的关系及内源性抗炎因子IL-10对致炎因子IL-1β是否有拮抗作用。方法:采集14例成人牙周炎患者炎症区牙龈组织,6例正常牙龈组织,利用逆转录-聚合酶链反应检测其中IL-1β与IL-10mRNA的表达及其强弱程度。结果:成人牙周炎组牙龈组织IL-1βmRNA的阳性表达率为92.86%,IL-10mRNA阳性表达率为71.43%,二者与正常对照组相比均有显著性差异;两者之间无明显相关性;两者与临床指标间亦无明显相关性。结论:致炎性和抗炎性细胞因子均可在牙龈组织中表达;机体自身IL-10水平不足以完全拮抗IL-1β活性;IL-10对致炎性细胞因子IL-1β的调控作用有待进一步研究。     

白细胞介素-33在牙周炎方面的研究进展

牙周炎是由牙周病原菌引起的牙周组织慢性炎症性、破坏性疾病,是牙齿缺失的主要原因。白细胞介素-33属于白细胞介素-1家族的新成员。白细胞介素-33参与牙周组织的炎性反应和免疫应答,在牙周炎的发生、发展过程中起重要作用。因此白细胞介素-33有望成为治疗牙周炎的新靶点。本文就白细胞介素-33的生物学特性以及其在牙周炎中的作用等方面的研究进展进行综述。     

白细胞介素- 8对牙周炎易感性的作用研究进展

白细胞介素-8(interleukin-8,IL-8)是一种中性粒细胞功能调节因子,对牙周炎的发生、发展具有重要影响.而牙周炎在一定程度上表现出明显的宿主易感性,与遗传基因关系密切.从基因水平研究IL-8对牙周炎易感性的作用有助于牙周炎的预防和早期控制.笔者从IL-8的基因结构、表达、致炎机制等方面对其在牙周炎遗传易感...     

白细胞介素-8对牙周炎易感性的作用研究进展

白细胞介素-8（interleukin-8,IL-8）是一种中性粒细胞功能调节因子,对牙周炎的发生、发展具有重要影响。而牙周炎在一定程度上表现出明显的宿主易感性,与遗传基因关系密切。从基因水平研究IL-8对牙周炎易感性的作用有助于牙周炎的预防和早期控制。笔者从IL-8的基因结构、表达、致炎机制等方面对其在牙周炎遗传易感性中的作用进行综述。     

IL-1βmRNA、TNF-αmRNA在成人牙周炎患者牙龈组织中表达的研究

目的:检测白细胞介素1B(IL-1B)mRNA、肿瘤坏死因子A( TNF-A)mRNA在成人牙周炎患者牙龈组织中表达, 探讨IL-1B和TNF-A与牙周炎致病机理的关系。方法:采用RT- PCR方法检测IL-1BmRNA、TNF-AmRNA在19例成人牙周炎患者炎症牙龈和9例牙周健康者牙龈组织中表达。结果:IL-1BmRNA表达强度在成人牙周炎病变牙龈 (0.819?01045)显著高于正常牙龈(0.306?0.087)(t=3.71,P<0.01)。TNF-AmRNA表达强度在成人牙周炎病变牙龈(0.696?0.098)显著高于正常牙龈(0)(t=7.09,P<0.01)。结论:IL-1B和TNF-A作为炎症和骨吸收介质可能在牙周炎的发生发展中起一定的作用。     

白细胞介素-21在不同牙周状态龈沟液中的表达及相关性分析

目的检测白细胞介素(Interleukin,IL)-21在不同牙周状态下龈沟液中的表达,探讨IL-21在牙周免疫中的可能作用。方法本研究共纳入47例患者,分为健康对照组(15例),轻度牙周炎组(10例),中重度牙周炎组(22例);记录患者一般信息、用Florida牙周探针检查牙周袋探诊深度(PD)和临床附着丧失(CAL)情况;收集牙周治疗前的龈沟液样本,采用酶联免疫吸附法(ELISA)测定不同牙周条件下龈沟液中IL-21的水平;并进行Pearson秩相关检验统计IL-21的表达与相关临床指标的关系。结果 3组龈沟液中IL-21平均浓度分别为(107.20±2.54)、(218.90±5.11)、(367.80±7.27)pg/m L,轻度牙周炎组及中重度牙周炎组龈沟液中IL-21水平显著高于对照组(P<0.05),其中以中、重度牙周炎组增高最为显著;中、重度牙周炎组龈沟液中IL-21水平分别与PD、CAL呈正相关关系(P<0.001)。结论 IL-21在牙周疾病中有较高的表达,尤其是以中重度牙周炎患者最为显著;由此推测IL-21在牙周疾病的发生发展中占有重要地位,与牙周组织的破坏有密切关系。     

牙周炎患者牙龈组织中IL-21表达的研究

目的：研究不同类型牙周炎患者牙龈组织中IL-21基因的表达,探讨其在牙周炎发病中的作用。方法：选择慢性牙周炎患者12例,侵袭性牙周炎8例,健康对照组8例,采用实时定量PCR方法定量检测IL-21 mRNA在牙龈组织表达情况。结果：慢性牙周炎、侵袭性牙周炎、健康对照组牙龈组织中均有IL-21 mRNA的表达,慢性牙周炎组和侵袭性牙周炎组IL-21 mRNA相对表达量分别为0.000534±0.000504和0.00602±0.000137,显著高于健康对照组0.000161±0.000352,差异有统计学意义(P<0.05)。慢性牙周炎和侵袭性牙周炎牙龈组织IL-21mRNA表达没有显著性差异（P>0.05）。结论：IL-21在慢性牙周炎发病机制中可能发挥重要作用。     

Increased interleukin-18 in gingival crevicular fluid from periodontitis patients

INTRODUCTION: This study aimed to measure the levels of interleukin-18 (IL-18) in inflamed shallow sites and inflamed deep sites in patients with periodontitis and to compare the data with results from inflamed shallow sites in patients with gingivitis. A secondary aim was to examine the composition of the subgingival microbiota in the sampled sites. METHODS: Gingival crevicular fluid was collected from five gingivitis sites and five periodontitis sites from 18 patients with chronic periodontitis, and from five gingivitis sites from 15 patients with gingivitis. Samples from each site category were pooled and IL-18 levels were measured using an enzyme-linked immunosorbent assay. The subgingival microbiota was analyzed by checkerboard DNA-DNA hybridization. RESULTS: All clinical parameters and gingival crevicular fluid volumes were higher in periodontitis sites compared with gingivitis sites from patients with periodontitis and gingivitis. The total amount of IL-18 was higher in periodontitis sites than gingivitis sites in both periodontitis (P = 0.018) and gingivitis (P = 0.002) patients and was higher in gingivitis sites from periodontitis patients than in those from gingivitis patients (P = 0.015). There were higher levels of Tannerella forsythia, Porphyromonas gingivalis, and Treponema denticola (red complex species) in periodontitis sites compared with gingivitis sites in both the periodontitis and gingivitis patients (P < 0.001). CONCLUSION: Levels of IL-18 were higher in patients with chronic periodontitis compared with patients with gingivitis, even at sites with similar pocket depths. The presence of similar levels of red complex species in gingivitis sites from periodontitis patients and from gingivitis patients suggested that the higher levels of IL-18 were not associated with a different microbial challenge.     

慢性牙周炎患者IL-17mRNA的表达及意义

目的：观察TL-17mRNA基因在慢性牙刷炎和健康牙龈组织中的表达水平变化,探讨TL-17在慢性牙周炎发生发展巾的作用。方法：选择慢性牙周炎患者23例,正常对照组15例,记录才周临床指标,采用Real—time PCR方法定量检测TL-17mRNA在牙龈组织中的表达。结果：慢性牙周炎组TL-17mRNA相对表达晕（0．00147＋0．00055）显著高于正常牙龈组（O．00047±0．00019）（P〈0．01）,并且慢性牙周炎组TL-17mRNA表达水平与牙龈指数（r=0．58,P〈0．01）、牙周袋探诊深度（r=0．57,P〈0．01）、附着丧失水平（r=0．49,P〈0．05）呈正相关。结论：Th17相关细胞凶子IL—17呵能住慢性牙周炎发病机制中发挥致炎作用。     

慢性牙周炎患者龈沟液中白细胞介素-4的检测和意义

目的检测慢性牙周炎患者牙周基础治疗前后龈沟液中白细胞介素-4(IL-4)的质量浓度,探讨IL-4与牙周炎的关系及其在牙周炎发病机制、病情进展等方面所起的作用。方法用滤纸条浸润法采集成年健康者和牙周炎患者治疗前后的龈沟液样本,用酶联免疫吸附测定检测样本中IL-4的质量浓度。结果慢性牙周炎患者龈沟液中IL-4的质量浓度低于健康对照组(P<0.05)。经牙周基础治疗1个月后,IL-4的质量浓度无明显变化,治疗前后的差异无统计意义(P>0.05);IL-4的质量浓度与探诊深度呈显著负相关,与牙龈指数和附着丧失无明显相关性。结论IL-4缺乏可能会导致牙周病的发生,IL-4可作为早期诊断牙周病和检测易患人群的敏感性指标。     

Effects of scaling and root planing on the amounts of interleukin-1 and interleukin-1 receptor antagonist and the mRNA expression of interleukin-1beta in gingival crevicular fluid and gingival tissues

OBJECTIVE: The purpose of this study was to evaluate the relationship between the clinical changes after non-surgical periodontal therapy and interleukin 1 (IL-1) in gingival crevicular fluid (GCF) and gingival tissues from patients with chronic periodontitis. BACKGROUND: The inflammatory responses mediated by IL-1 play an important role in periodontal tissue destruction. Although numerous studies have attempted to elucidate the dynamic movement involved in chronic periodontitis, the results have often conflicted. Such discrepancies may have been due to the inability to determine clinical disease activity. METHODS: Seven patients with chronic periodontitis were examined. The severity of periodontal inflammation was expressed using clinical parameters before and after a scaling and root planing (SRP) procedure. The amounts and concentrations of IL-1alpha, IL-1beta and IL-1 receptor antagonist in GCF were measured by enzyme-linked immunosorbent assay (ELISA) and IL-1 activity index was calculated. A needle biopsy in matching gingival tissues was also performed before and after the SRP procedure. The localization and mRNA expression of IL-1beta were determined using histological methods. RESULTS: Clinical parameters improved slightly after the SRP procedure. Only the probing pocket depth (PPD) was reduced significantly (p < 0.05). However, the amount of IL-1beta in GCF was slightly increased. The localization and mRNA expression of IL-1beta could still be observed after the SRP procedure. Therefore, none of the clinical parameters showed a high sensitivity or specificity for evaluating subgingival inflammation. CONCLUSION: These observations suggest that IL-1 is effective for evaluating in detail the state of subgingival inflammation.     

The interleukin-10 promoter haplotype ATA is a putative risk factor for aggressive periodontitis

Background and Objective:  Interleukin-10 has been described as an anti-inflammatory cytokine and a B-cell proliferation factor. Promoter polymorphisms of the interleukin-10 gene have been associated with altered interleukin-10 expression. Therefore, the aim of this study was to evaluate three polymorphisms at positions −1082G>A, −819C>T and −590C>A in patients with generalized chronic periodontitis ( n  = 27) and generalized aggressive periodontitis ( n  = 32) in comparison with periodontitis-free controls ( n  = 34).
Material and Methods:  Interleukin-10 promoter polymorphisms were analyzed by polymerase chain reaction with sequence-specific primers (PCR-SSP). Distributions of single alleles, genotypes and haplotypes were calculated by the chi-square test. Risk factor analyses were carried out by logistic regression. Subgingival bacteria were subjected to molecular biological analyses using the micro-Ident^® test.
Results:  The combination ATA/ATA was found only in patients with aggressive periodontitis (15.6 vs. 0.0%, p  = 0.023). Taking into account age, gender, smoking and plaque level, an increased odds ratio (3.7, p  = 0.04) for aggressive periodontitis was shown for subjects with the haplotype ATA. Prevotella intermedia was found to be decreased in ACC- positive (41.3 vs. 66.7%, p  = 0.022), ATA-positive (33.3 vs. 57.1%, p  = 0.032) and ACC/ATA-positive (20.0 vs. 55.9%, p  = 0.002) individuals. In GCC/GCC-positive subjects, P. intermedia occurred more frequently (86.7 vs. 42.3%, p  = 0.002).
Conclusion:  The haplotype ATA, which is known as a 'low interleukin-10 producer' is a putative risk indicator for generalized aggressive periodontitis.     

广泛型侵袭性牙周炎患者龈沟液中白介素-17的检测

目的 比较广泛型侵袭性牙周炎患者与健康人龈沟液中白介素-17(IL-17)的表达水平及与临床指标的关系。方法 选择广泛型侵袭性牙周炎患者18例和健康人16例,共68颗牙,记录临床牙周检查指标,用滤纸条法收集龈沟液。采用抗体夹心ELISA法测定广泛型侵袭性牙周炎患者治疗前、治疗后3个月及健康对照者龈沟液中IL-17总量和浓度。结果 广泛型侵袭性牙周炎患牙治疗前龈沟液中IL-17的总量和浓度高于牙周健康牙,基础治疗3个月后广泛型侵袭性牙周炎患牙龈沟液中IL-17的总量和浓度较治疗前下降。广泛型侵袭性牙周炎患牙龈沟液中IL-17浓度与探诊深度、附着丧失和出血指数呈正相关关系,而IL-17总量仅与探诊深度呈正相关关系。结论 龈沟液中IL-17浓度可能与广泛型侵袭性牙周炎牙周破坏及炎症的严重程度相关。?     

Immunohistological analysis of gingival lymphocytes in adult periodontitis

Inflammatory periodontal diseases are mediated by interactions between the dental plaque and the components of the host immune system. This study was designed to analyse the phenotypic properties of gingival lymphocytes in adult periodontitis. Biopsies were obtained from 12 patients and aged between 35 and 55 years. The tissues were processed for both histopathological and immunohistological examinations. Gingival tissue lymphocytes were identified using monoclonal and polyclonal antibodies with the immunoperoxidase technique. All specimens revealed a significant degree of CD3(+) cell infiltration beneath the pocket epithelium, which is located adjacent to the bacterial plaque, compared to that on the oral epithelial side. CD4(+) and CD8(+) cells were evenly distributed within these infiltrates. Numerous HLA-DR(+) cells were also noted. The majority of plasma cells in the central lamina propria bore IgG isotypes. These findings suggest that T-cell mediated regulatory mechanisms play an important role in the pathogenesis of adult periodontitis.     

Interleukin-10 gene promoter polymorphism in Japanese patients with adult and early-onset periodontitis

BACKGROUND: IL-10 is an anti-inflammatory cytokine, which may modulate disease expression in chronic inflammatory periodontal disease. 3 dimorphic polymorphisms within the IL-10 gene promoter have recently been identified and appear to influence regulation of its expression. AIM: The aim of the present study was to investigate whether the promoter polymorphisms are associated with adult periodontitis (AP) and generalized early-onset periodontitis (G-EOP). METHODS: Genomic DNA was obtained from 34 AP patients, 18 G-EOP patients and 52 controls. The promoter region between -506 and -1140 was amplified by polymerase chain reaction, and polymorphisms were detected by nucleotide sequencing. RESULTS: The haplotype frequencies in Japanese were quite different from those of Caucasian and were even slightly different from those of southern Chinese with systemic lupus erythematosus. We found no significant difference in allele or haplotype frequencies between patients and controls. CONCLUSIONS: IL-10 production may be regulated within the complex cytokine network in chronic inflammatory periodontal disease, rather than the gene polymorphisms.