7圈滑行成绩并血乳酸值对运动训练及运动员功能状态的监控作用

目的通过少儿短道速滑运动员7圈滑行成绩与血乳酸值来评定竞技水平，对提高运动员运动训练水平有着重要实践价值。方法由教练员指挥控制的7圈滑行，强度要求本人最大摄氧量100％～120％，滑行结束后3～5min取末稍血测定乳酸值，同时记录成绩。结果7圈滑行后血乳酸可达到，男6．3～9．6mmol／L，女：5．8～11mmol／L；成绩分别为男73．40～80．31s，女：74．93～80．79s。结论7圈滑行是评定短道速滑运动员竞技水平可靠手段，配合血乳酸的测试，对更客观评定运动员竞技水平及功能状态起到科学互补作用。     

血乳酸监测与心外科病人的病情评估

血乳酸作为体内代射和组织内部氧供求关系的标志,其准确监测对心外科病人病情评估及预后评估有重要意义.但其监测值受采血部位、采血方法及标本送检时间等的影响.     

血乳酸监测与心外科病人的病情评估

血乳酸作为体内代射和组织内部氧供求关系的标志，其准确监测时心外科病人病情评估及预后评估有重要意义。但其监测值受采血部位、采血方法及标本送检时间等的影响。     

新生儿动脉血与末梢血血细胞计数结果的比较

随着医学水平的不断提高,检验技术日益完善,血细胞计数逐步由末梢血向静脉血检测转变,采用静脉血检测血细胞计数不仅结果准确,便于复查,其稳定性也优于末梢血,儿童静脉血与末梢血细胞各项参数有区别[1]。由于新生儿是特殊人群,生理条件变化较大,为了给临床提供更加准确的分析参数,我们对新生儿动脉血与末梢血的各项参数进行分析,旨在为新生儿血细胞计数选择较为合适的采集方法。     

4种便携式血糖仪与生化分析仪的一致性和抗干扰能力的研究

目的 通过对不同便携式血糖仪与贝克曼库尔特A U 5800全自动生化分析仪(A U 5800)检测血糖结果的一致性比对和抗干扰能力的研究,为临床选择适合的血糖仪提供参考.方法 共选取4种不同检测原理的2台进口(A和B)和2台国产(C和D)血糖仪,与A U 5800同时检测不同浓度的血糖并进行比较,分析其一致性.分别检测...     

便携式血糖监测仪与生化分析仪检测糖尿病患者血糖的结果分析

目的 比较便携式血糖监测仪与生化分析仪两种检测方法对糖尿病患者血糖检测结果的差异.方法 回顾性分析曲靖市第一人民医院内分泌科住院的120例糖尿病患者应用便携式血糖监测仪与生化分析仪检测空腹血糖及早餐后2h血糖检测结果的差异.结果 两组患者空腹血糖及餐后血糖便携式血糖监测仪检测结果稍低于生化分析仪检测结果,差异有统计学意义(P＜ 0.05),但相关系数γ=0.978,提示两种检测方法有相关性.结论 便携式血糖监测仪与生化分析仪检测糖尿病患者的空腹血糖及餐后血糖前者稍低于后者,但结果具有相关性,便携式血糖监测仪检测血糖简便、快速、准确,可用于糖尿病患者自我监测血糖.     

血液分析仪测定静脉血与末梢血的比较

过去，用手工方法测定血液常规大都用末梢血．随着全自动血液分析仪的临床应用，用静脉抗凝血越来越多。因此．给临床护理增加了负担。用静脉血还是末梢血做血常规．各家说法不一．为此我们用CD—1600血液分析仪对40例病人．同时取静脉血和末梢血进行测定比较．现将结果报告如下。     

124例指血与静脉血18项血液参数结果比较

指血与静脉血的血细胞检查结果有无差异是大家非常关注的问题。作者对１２４例门诊患者的指血与静脉血１８项血细胞参数进行了比较，现报道如下。１材料与方法１．１仪器与试剂雅培ＣＤ－１６００型血细胞分析仪及进口配套试剂。仪器批内重复性测试ＣＶ值ＷＢＣ２％，ＲＢ...     

便携式血糖仪与生化分析仪血糖检测结果的相关性研究

目的 通过对三台不同型号便携式血糖仪与生化分析仪血糖检测结果进行比对和偏差分析,了解血糖仪与生化仪血糖检测结果的相关性.方法 参照《医疗机构便携式血糖检测仪管理和临床操作规范(试行)》中静脉血样比对试验方案,收集50例不同血糖浓度患者静脉全血样本,血糖仪测定全血葡萄糖,剩余样本立刻离心分离血浆,30 min内用生化仪测定血浆葡萄糖,计算血糖仪与生化分析仪测定结果的相关系数和偏差.结果 三台血糖仪批内CV在1.28％～4.87％之间;日间不精密度低浓度质控品s ＜0.13 mmol/L,中、高浓度质控品CV 在0.81％～2.14％之间,符合《规范》中精密度的要求.三台血糖仪与生化仪测定结果的相关系数r均＞0.98.回归方程斜率b＞1.0±0.05的理想范围.血糖浓度2.3～4.2 mmol/L的样本,稳步、卓越和活力血糖仪只有60％,30％和80％检测结果误差在±0.83 mmol/L的范围内;血糖浓度4.6～22.6 mmol/L样本,有95％～97.5％检测结果误差在允许范围内.结论 三台血糖仪精密度符合《规范》要求,与生化分析仪血糖测定结果有较好相关性.血糖仪的低浓度样本(血糖＜4.2 mmol/L)误差不符合《规范》要求,必要时抽取静脉血生化仪复查;中、高浓度样本误差符合《规范》要求.     

便携式血气分析仪与生化分析仪在检测电解质方面的对比研究

目的比较血气分析仪和自动生化分析仪对电解质检测结果的差异,为临床判断提供依据。方法观察100例重症监护病房患者,同时抽取静脉血和动脉血分别应用自动生化分析仪和血气分析仪检测电解质结果;数据采用SPSS13.0统计软件分析。结果通过对100对样本分析,动脉血气分析仪（ABG）检测钾离子结果（3.69±0.55）mmol/L,自动生化分析仪（AA）检测钾离子结果（3.84±0.57）mmol/L,差异有统计学意义（P〈0.01）;ABG检测钠离子结果为（141.53±9.86）mmol/L,AA检测钠离子结果为（142.72±8.60）mmol/L,差异无统计学意义（P〉0.05）。结论危急情况下可以依据动脉血气分析仪检测的钠离子结果,及时作出病情判断及处理。     

三种仪器检测血糖的对比分析

目的 研究利用血气分析仪与便携式血糖仪以及全自动生化分析仪测定血糖的相关性与偏倚,并探讨其差异.方法 分别用GEM Premier3000血气分析仪、罗氏罗康全型便携式血糖仪及日立7080全自动生化分析仪对40例患者的动脉血液标本进行血糖浓度的检测,对检测结果做统计学处理,观察三者结果差异有无统计学意义,并分析原因.结果 GEM Premier3000血气分析仪、罗氏罗康全型血糖仪与日立7080生化仪测定值间差异均无统计学意义(P>0.05).结论 同一实验室的检测系统应进行方法学对比和结果偏差评估,从而判断其临床可接受性,以保证检验结果的可比性.     

Analytical performance of a portable critical care blood gas analyzer

The portable blood gas analyzer OPTI Critical Care Analyzer was evaluated in comparison to routine laboratory assays using heparinized blood samples of adults and newborns. Within-run imprecision studies were performed with native blood using tonometry to adjust blood gas concentrations. The results obtained show a very close agreement between the OPTI system and the comparison methods for all parameters tested: hemoglobin (y=1.00x-0.2 g/l, r=0.997, n=81), sodium (y=1.13x-18.7 mmol/l, r=0.951, n=79), potassium (y=1.03x-0.04 mmol/l, r=0.972, n=79), pH (y=1.03x-0.29, r=0.958, n=57), pCO2 (y=1.03x-1.14 mm Hg, r=0.988, n=57) and pO2 (y=1.07x-0.85 mm Hg, r=0.995, n=57). The coefficients of variation for the within-run imprecision were below 1.1% for sodium and hemoglobin, and below 2.6% for all other parameters, except for pCO(2) with coefficients of variation up to 3.6% at low calibration gas concentrations. Due to this analytical performance and its portability, the OPTI system is well suited for low to medium test frequencies and immediate use in emergency rooms, intensive care or surgery units.     

Evaluation of a portable blood gas analyzer for pediatric interhospital transport

We evaluated a portable blood gas analyzer for its speed, reliability, and usefulness during interhospital transportation of critically ill children in a prospective study. The accuracy of a portable blood gas analyzer (PBGA) was first established by comparing its results with values obtained from a standard blood gas analyzer. The speed, accuracy, and usefulness of the PBGA were then compared with those of standard analyzers at 10 referring hospitals during interhospital transportation of critically ill children. A highly significant linear correlation was demonstrated between values obtained using the PBGA and those derived from standard analyzers. The time required to obtain blood gas results was 2 min with the portable device, significantly less than the mean of 8.4 min ± 6.4 min (range 1–24 min) required to get results from the laboratory facilities of the referring hospitals. Modification of treatment or adjustment to mechanical ventilation was required in 30% of transported patients based on blood gas results obtained immediately before departure from the referring hospital. We conclude that a portable blood gas analyzer provides rapid, reliable, and useful data that help to determine therapy for critically ill children awaiting interhospital transport.     

Lactate analyzer with continuous blood sampling for monitoring blood lactate during physical exercise

To monitor changes in the concentration of blood lactate during physical exercise, we used an automated lactate analyzer based on an electro-enzymatic method with continuous blood sampling through a catheter. The lactate concentration was measured every 2 min; between measurements, the instrument was calibrated with a lactate standard. Ascorbic acid, bilirubin, hemoglobin, creatinine, uric acid, and glucose did not interfere with the measurements. The lactate concentrations in blood samples from apparently healthy subjects before and after exercise correlated well (r = 0.993) with results by the conventional enzymatic method. We measured the blood lactate concentrations in nine apparently healthy volunteers during exercise on a treadmill with an increasing workload. The point at which lactate concentrations started to increase was detected easily. Thus, the lactate analyzer is suitable for monitoring changes in blood lactate concentrations during exercise.     

血液分析仪白细胞分类报警阈值的ROC曲线分析

目的应用受试者工作特征(ROC)曲线对CELL-DYN 3700血液分析仪白细胞分类结果进行分析,建立本室最佳报警参考阈值。方法将238例患者静脉血标本以EDTA-K2抗凝,分别用仪器和显微镜进行白细胞分类,以SPSS 10.5统计软件的ROC曲线对5种分类参数不同阈值进行分析,选择曲线下面积(AUC)最大者为本实验室报警阈值,并与仪器设置阈值进行比较。结果经ROC曲线分析,仪器设置各参数阈值分别为中性粒细胞(N)<50%或>75%、淋巴细胞(L)<20%或>40%、单核细胞(M)>12%、嗜酸性粒细胞(E)>5%、嗜碱性粒细胞(B)>2%诊断试验整体准确性中等(AUC=0.792);当N>80%或N<20%、L>50%或L<10%、M>10%、E>5%、B>1.5%时AUC最大(AUC=0.926),诊断试验整体准确性优秀。最佳报警阈值对显微镜分类正常或异常标本判别的敏感度、特异度和准确度分别为0.91、0.90和0.91,明显优于仪器设置阈值。结论实验室应建立自己的报警阈值,以提高显微镜协同工作的有效性。     

血液分析仪白细胞分类报警阈值的ROC曲线分析

目的应用受试者工作特征（ROC）曲线对CELL—DYN3700血液分析仪白细胞分类结果进行分析,建立本室最佳报警参考阈值。方法将238例患者静脉血标本以EDTA—K2抗凝,分别用仪器和显微镜进行白细胞分类,以SPSS10．5统计软件的ROC曲线对5种分类参数不同阈值进行分析,选择曲线下面积（AUC）最大者为本实验室报警阈值,并与仪器设置阈值进行比较。结果经ROC曲线分析,仪器设置各参数阈值分别为中性粒细胞（N）〈50％或〉75％、淋巴细胞（L）〈20％或〉40％、单核细胞（M）〉12％、嗜酸性粒细胞（E）〉5％、嗜碱性粒细胞（B）〉2％诊断试验整体准确性中等（AUC=0．792）;当N〉80％或N〈20％、L〉50％或L〈10％、M〉10％、E〉5％、B〉1．5％时AUC最大（AUC=0．926）,诊断试验整体准确性优秀。最佳报警阈值对显微镜分类正常或异常标本判别的敏感度、特异度和准确度分别为0．91、0．90和0,91,明显优于仪器设置阈值。结论实验室应建立自己的报警阈值,以提高显微镜协同工作的有效性。     

Multiple site analytical evaluation of a portable blood gas/electrolyte analyzer for point of care testing

OBJECTIVE: To evaluate the analytical performance of the SenDx 100 portable blood gas and electrolyte analyzer (SenDx Medical, Carlsbad, CA). DESIGN: Accuracy was evaluated by correlation of whole blood patient samples with the Nova Stat Profile 5 (Nova Biomedical, Waltham, MA) and the Ciba Corning 865 (Chiron Diagnostics, Medford, MA). Precision was evaluated using quality control materials (RNA Medical, Acton, MA). SETTING: Critical care laboratories and operating rooms in two institutions. MEASUREMENTS AND MAIN RESULTS: Precision studies performed at three different concentration levels for each analyte demonstrated intra-assay precision of < or =2.5% coefficient of variation and interassay precision of < or =4.0% coefficient of variation in all cases. Analysis of patient specimens in general showed good to excellent correlation to reference analyzers. Regression variables are tabulated. CONCLUSIONS: The SenDx 100 portable blood gas and electrolyte analyzer is a simple and easy to use analyzer demonstrating acceptable performance compared with reference methods.