口腔粘膜病损HPV的检测

应用ＰＣＲ技术检测口腔粘膜病损中ＨＰＶ的感染情况。病损包括白斑、扁平苔藓、慢性盘状红斑狼疮、日光性唇炎、鳞癌及鳞癌术后上皮高度增生共３４例。结果表明在口腔癌与白斑中斑可存在ＨＰＶ的感染，ＨＰＶ可能是口腔癌的致病因子之一。     

口腔扁平苔藓人乳头瘤病毒的检测

目的 探讨口腔扁平苔藓（OLP）和人乳头瘤病毒（HPV）的关系，从而为确定OLP的原因，诱因和发病机理提供理论依据。方法 应用通用引物（General Primer,GP)介导的PCR技术检测OLP和正常口腔黏膜的HPVDNA，并对HPV－16DNA进行特异性检测。结果 30例OLP检测到25例HPV－6，11，33DNA阳性，而对照组检测到5例，两者相比有显著性差异（P＜0．05）；OLP组检测到3例HPV－16，18DNA阳性，对照组1例，两者相比无显著性差异（P＞0．05）。结论 OLP是以低危型HPV感染为主，其中糜烂型OLP可检测到较高高危HPV16 DNA。因此，对OLP尤其是糜烂型OLP要进行HPV的检测，从而进行有效治疗。     

乳头状瘤病毒与口腔粘膜病损关系研究现状

早在50年前,有关口腔乳头状瘤与病毒的关系首先在动物体内得到了证实。目前,对乳头状瘤病毒(papillomaviruses,PVs)在人体口腔粘膜病损方面所起的作用尤为关注。最近发现该病毒与各种疣状损害、癌前病变和恶性上皮瘤的病因学相关,本文就乳头状瘤病毒的基本特性及其在口腔粘膜良性病变、癌前病损和一些口腔癌的发生发展中可能起到的作用作一综述。一、基本特性 1、性质和种类: 乳头状瘤病毒属乳头状瘤多型空泡形病毒A亚群。本病毒是一些最小的DNA病毒(直径为50—55nm),无包膜,含有蛋白及超螺旋单环双股DNA;其二十面体微粒含72质粒。PVs与大多数     

口腔粘膜组织中HPV16DNA和空泡细胞关系的研究

本研究采用斑点杂交技术，检测８３例口腔扁平苔藓、口腔白斑、口腔鳞癌及正常口腔粘膜组织中的ＨＰＶ１６ ＤＮＡ，并分析ＨＰＶ１６ ＤＮＡ与空泡细胞出现的关系。口腔粘膜组织ＨＰＶ１６ ＤＮＡ的检出率为１０．８％，空泡细胞的检出率为３４．９％。两者均阳性的检出率为７．２％，结果提示空泡细胞不是ＨＰＶ感染所特有的，不能作为诊断ＨＰＶ的特征性指标。     

正常口腔粘膜组织中人类乳头状瘤病毒的研究现状

人类乳头状瘤病毒(HPV)目前已鉴定60余型,与正常口腔粘膜相关的有HPV 4,6,11,16,16-R18,31,33等,它以低拷贝、潜伏状态存在于上皮组织内。漱口、搔刮比活检方法所获标本的阳性率更高GP-PCR是检测正常口腔粘膜HPV的敏感方法。     

口腔扁平苔藓中人乳头瘤病毒的检测

为研究口腔扁平苔藓（OLP）与人乳头瘤病毒（HPV）的关系．作者采用聚合酶链（PCR）技术，对22例OLP中的HPV低危型──11型以及高危型—16型DNA分别进行了检测，并以10例正常口腔粘膜（NOM）作为对照。结果表明：PCR是检测OLP中HPV较为理想的方法。OLP中的HPV与NOM相比较，11型相对升高。OLP各临床分型中，网纹型的HPV阳性百分比最高，该型的病程迁延．治疗后易复发，可能与HPV的潜伏和感染有关，4例糜烂型，即有2例含有HPV16，提示糜烂型与癌变的关系。HPV在NOM中的存在。表明HPV可以无症状或以潜伏的形式存在于口腔粘膜。     

HPV感染与口腔癌——口腔癌诊疗思路的变革

人乳头瘤病毒（Human papilloma virus HPV）是一种嗜黏膜和皮肤上皮的DNA病毒。自20世纪70年代发现宫颈癌的发生与HPV有关,人们对其进行了大量的研究。研究的进展之一是HPV感染与头颈部肿瘤,特别是与口咽癌（包括扁桃体癌和舌根癌）密切相关。并且,HPV相关口腔癌患者数目的大量上升,引起了头颈肿瘤医生,特别是口腔颌面外科医生们的高度关注。     

正常口粘膜组织中人类乳头状瘤病毒的研究现状

人类乳头状瘤病毒目前已鉴定６０余形，与正常口腔粘膜相关的有ＨＰＶ４，６，１１，１６，１６－Ｒ，１８，３１，３３等，它以低拷贝、潜伏状态存在于上皮组织内。漱口、搔刮比活检方法所荻标本的阳性率更高。     

人口腔黏膜角化上皮细胞的体外培养及生物学特性研究

目的:探讨人口腔黏膜角化上皮细胞的体外培养扩增方法及其生物学特性。方法:取牙周手术中切取的正常角化牙龈组织,经0.25％Dispase分离上皮层后,0.05％的胰酶分离为单个细胞,接种在无血清培养基中,进行原代培养及传代培养,并进行形态学观察、角蛋白免疫组化染色及其生长曲线、传代特点、各代生长特点的观察。结果:口腔黏膜角化上皮细胞能够在Epilife无血清培养基中稳定增殖传代,可连续传4-6代,成活45-60d,细胞生长呈铺路石状,角蛋白免疫组化染色阳性。结论:应用Epilife无血清培养基,可在短期内获得大量具有增殖能力的口腔黏膜角化上皮细胞。     

Detection of human viruses in periodontal pockets using polymerase chain reaction

Even though viruses have been implicated in the etiology of several medical and dental disorders, little or no data are available on the possible involvement of human viruses in the pathogenesis of human periodontal disease. This study investigated the presence of human cytomegalovirus, Epstein-Barr virus, herpes simplex virus, human papillomavirus and human immunodeficiency virus (HIV) in crevicular fluid samples from 30 patients with advanced periodontitis and 26 subjects with gingivitis. Viral identification was performed on direct subgingival samples from 3 diseased sites in each patient using the polymerase chain reaction technique. Seventy-eight percent of advanced periodontitis patients were positive for at least one of the five test viruses. Cytomegalovirus was detected in 60% of the periodontitis patients, Epstein-Barr virus in 30%, herpes simplex virus in 20%, human papillomavirus in 17% and HIV in 7%. Forty percent of the periodontitis patients revealed coinfection by 2 to 5 viruses. Only 31 % of the gingivitis subjects showed a positive viral identification in crevicular fluid, and infected individuals only revealed human cytomegalovirus. This study demonstrated that human viruses may occur in periodontitis lesions with relatively high prevalence. The pathogenetic significance of human viruses in destructive periodontal disease needs to be determined.     

正常口腔黏膜和口腔鳞癌中人乳头瘤病毒感染率的研究进展

黏膜高危型HPV-16、HPV-18感染是宫颈癌的主要致病因素,且与口腔鳞癌的发生密切相关,但目前对口腔鳞癌中HPV的感染率和亚型的分布尚不十分清楚。作者系统查阅了目前已经发表的有关HPV在正常口腔黏膜或口腔鳞癌中感染率和亚型分布的文献资料,对正常口腔黏膜、口腔鳞癌中人乳头瘤病毒的感染率的研究进展进行了综述。     

口腔粘膜嗜酸性溃疡的临床病理分析

目的 探讨口腔粘膜嗜酸性溃疡的病因、临床病理表现、诊疗与预后,以期对该病有所了解和认识。方法 对８例口腔粘膜嗜酸性溃疡病人进行临床病理分析。结果 口腔粘膜哮酸性溃疡界限清楚,表面清洁,所有病例均正规闰于舌部且为单发,３例有复发史,镜下以固有层嗜酸性细胞为特点。结论 口腔粘膜嗜酸性是一种少见的自限性良性疾病,好于发老人和儿童,病因不清,可能与创伤有关,诊断根据临床病理表现,治疗采用活检切除产完全切除     

人乳头状瘤病毒16/18型在口腔疣状癌中的表达及意义

目的　研究人乳头状瘤病毒 16 /18型在口腔疣状癌中的表达状况 ,探讨其在口腔疣状癌发生发展中的生物学意义。方法　采用SP免疫组化和原位杂交方法分别检测 8例正常口腔粘膜、13例疣状癌、10例高分化鳞状细胞癌、10例低分化鳞癌组织中HPV16 /18E6蛋白和HPV16 /18DNA的表达。结果　①疣状癌HPV16 /18E6蛋白及HPV16 /18DNA阳性表达率均为 6 9.2 % (9/13) ,E6蛋白平均染色强度高于高分化鳞状细胞癌和低分化鳞状细胞癌组 (P <0 .0 5 )。②免疫组化方法检测HPV16 /18E6蛋白与原位杂交方法检测HPV16 /18DNA结果有良好的一致性。结论　HPV16 /18型感染是口腔疣状癌的重要致病因子 ,与高分化鳞状细胞癌、低分化鳞状细胞癌组织相比 ,HPV16 /18型感染与疣状癌的关系更为密切。     

Detection of Epstein-Barr virus and human papillomavirus type 16 DNA in hairy leukoplakia by in situ hybridisation and the polymerase chain reaction

Demonstration of Epstein-Barr virus (EBV) is considered desirable for the accurate diagnosis of hairy leukoplakia (HL). Previous studies have reported possible associations with human papillomavirus (HPV) infection although this is not a universal finding. Presence of EBV and HPV 16 was examined in biopsy specimens from 18 cases of HL and ten control specimens by in situ hybridisation using digoxigenin-labellcd synthetic oligonucleotide probes and by the polymerase chain reaction (PCR). The presence of EBV was demonstrated in 12 cases by both techniques. Of the remaining six cases EBV could be detected in three by in situ hybridisation but not by PCR; EBV was not detected by either method in a further three cases. All samples were negative for HPV 16 by both techniques under conditions of high stringency, although when stringency of in situ hybridisation was reduced, four samples appeared to harbour HPV DNA sequences. This study provides further evidence to support the role of EBV in the pathogenesis of HL and suggests that HPV 16 is not regularly encountered.     

慢病毒介导过表达端粒酶逆转录酶的 人口腔黏膜上皮稳定细胞系的构建

目的 通过慢病毒法建立稳定表达外源性人端粒酶逆转录酶（hTERT）基因的口腔黏膜上皮细胞（OMECs）,探索构建高效、稳定的永生化OMECs细胞系的方法。方法 提取293T细胞总RNA,应用聚合酶链反应（PCR）法扩 增hTERT基因全长,构建重组慢病毒载体pLVX-puro-hTERT。包装慢病毒颗粒后感染人正常OMECs,经嘌呤霉素抗 性筛选获得阳性克隆,采用实时荧光定量PCR法和Western blot法检测hTERT基因mRNA和蛋白的表达水平。结果 成功构建了pLVX-puro-hTERT过表达慢病毒载体并感染到OMECs中;感染细胞与正常OMECs形态相似,呈铺路石 样生长;实时荧光定量PCR和Western blot结果均显示,hTERT在感染细胞中高表达,与正常细胞相比差异有统计学 意义（P<0.05）。结论 通过慢病毒法成功建立了过表达hTERT的OMECs稳定细胞系,为构建高效、稳定增殖的人 永生化OMECs细胞系奠定了实验基础。     

正常人口腔粘膜不同部位表面感觉的测试

目的 建立正常人口腔粘膜不同部位表面感觉的正常参考值，并测试各年龄组感觉值是否存在差异。方法 利用动静两点辨别觉试验，测试90例健康人口腔粘膜不同部位的表面感觉。结果 舌尖感觉功能最好，其次为舌背、舌腹、颊粘膜、口底和硬腭。健康人的口腔粘膜不同部位的表面感觉随着年龄增长有显著性变化。结论 口腔粘膜不同部位感觉辨别力存在差异，健康人口底、舌腹、舌背、颊和硬腭粘膜感觉功能有增龄性变化。     

Human papilloma virus in erosive oral lichen planus

Several types of human papilloma viruses (HPV) have been associated with benign and malignant squamous cell tumours of mucosal epithelium. To identify HPV in erosive oral lichen planus (OLPe), considered as a premalignant lesion, tissues from 20 patients were examined by Southern blot hybridization with 32P-labeled HPV DNA probes. Type 11 was found in 6 of the lesions while HPV types 6, 16 and 18 were not detected in any of the tissues examined. Using a type-specific polymerase chain reaction (PCR) assay for HPV-6, 11, 16 and 18, HPV-11 was detected in 8 of the samples (all of those positive by Southern blot), and, in addition, HPV-6 was found in 5 samples and HPV-16 in 3 samples. Overall, by the more sensitive PCR assay, 65% of samples were positive for HPV DNA. The finding of HPV DNA in many of the samples using two different techniques indicates a high prevalence of HPV in the OLPe afflicted oral mucosa. However, the role of HPV in the pathogenesis of OLPe has yet to be determined.     

Herpes simplex virus type 1 and type 2 infection in human oral mucosa in culture

To examine the sensitivity of human oral mucosa to herpes simplex virus type 1 (HSV-1) and type 2 (HSV-2) infection, human gingival mucosa explants were infected with either HSV-1 or HSV-2 in vitro and the expression of virus specific antigen was examined by the immunofluorescent antibody technique. HSV-2 antigen was found in the basement membrane, basal cell layer and lower prickle cell layer. This finding was consistent with the HSV-1 infection. Electron microscopic study revealed the presence of nucleocapsids and enveloped virus particles in the basal cells of HSV-2-infected organ cultures. These findings indicate that human gingival mucosa is sensitive to infection with HSV-2, as well as HSV-1, and that the virus may replicate in the undifferentiated epithelial cells of mucosal epithelium.