空心加压螺钉固定加带蒂大转子骨瓣移植治疗GardenⅢ、Ⅳ型股骨颈骨折

目的 探讨空心加压螺钉固定加带蒂大转子骨瓣移植治疗GardenⅢ、Ⅳ型股骨颈骨折的治疗价值。方法应用带蒂大转子骨瓣移植并空心加压螺钉固定术，治疗GardenⅢ、Ⅳ型股骨颈骨折共23例，其中新鲜骨折2l例，陈旧骨折2例。结果平均随访1．5年～4年，随访X线片显示，22例骨折愈合，股骨头正常，1例陈旧骨折发生股骨头坏死。结论空心加压螺钉固定加带蒂大转子骨瓣移植治疗GardenⅢ、Ⅳ型股骨颈骨折术，可促进股骨颈骨折愈合，有效预防股骨头坏死。     

加压螺纹钉和带臀中肌蒂骨块移植治疗股骨颈骨折的临床观察

目的　探讨加压螺纹钉加带臀中肌蒂大转子骨块移植治疗青壮年股骨颈骨折的方法和疗效。方法　采用加压螺纹钉内固定加带臀中肌蒂骨块移植治疗青壮年股骨颈骨折 4 7例。结果　术后随访 2年～ 5年 2个月 ,平均时间 3年 5个月 ,所有病例骨折均达到解剖复位 ,无骨不连发生 ,发生股骨头缺血性坏死 2例 ,手术治疗优良率达 91 5 %。结论　该术式能提高青壮年股骨颈骨折的愈合率 ,避免发生骨不连和股骨头缺血性坏死等严重并发症。     

带旋髂深血管蒂髂骨瓣联合骨髓移植治疗GardenⅢ、Ⅳ型股骨颈骨折

目的:回顾性总结分析带旋髂深血管蒂髂骨瓣加骨髓移植治疗GardenⅢ、Ⅳ型股骨颈骨折的疗效.方法:对39例GardenⅢ、Ⅳ型股骨颈骨折采用带旋髂深血管蒂髂骨瓣联合红骨髓移植治疗,同时应用3根空心加压螺纹钉内固定.结果:术后随访9～35个月,39例在术后6个月至1年全部骨折愈合,4例出现股骨头坏死,优良率达89.7%.结论:带旋髂深血管蒂髂骨瓣具有充足血供,红骨髓具有很强成骨作用,结合坚强内固定能明显提高股骨颈骨折的治愈率,减少股骨头无菌性坏死的发生,对治疗GardenⅢ、Ⅳ型股骨颈骨折具有重要价值.     

空心钉加股方肌骨瓣移植治疗股骨颈骨折

目的:探讨用空心加压螺纹钉加股方肌骨瓣移植治疗股骨颈骨折的方法和疗效. 方法:回顾分析35例股骨颈骨折经多枚空心加压螺纹钉内固定,并行带股方肌肌蒂骨瓣移植的治疗. 结果:术后随访1～4年,32例骨折愈合,1例骨折不愈合,2例发生股骨头坏死. 结论:该术式能提高股骨颈骨折的愈合率,减少发生骨不连和股骨头缺血坏死等并发症,是治疗股骨颈骨折较好的手术方法.     

带缝匠肌蒂髂骨瓣移植加空心加压螺纹钉内固定术治疗青壮年股骨颈骨折45例中长期随访

目的 回顾性分析移植带缝匠肌蒂髂骨瓣加空心加压螺纹钉内固定术治疗青壮年股骨颈骨折的疗效及术后注意事项.方法 自1999年9月至2006年2月对我院45例青壮年股骨颈骨折行空心加压螺纹钉内固定加带缝匠肌髂骨瓣移植术后回顾性分析,结果有35例获得随访,平均随访6年2个月,除1例骨折延迟愈合,1例患肢短缩2厘米伴有约6%股骨头局部囊变(2个月股骨颈陈旧性骨折)外,其余均无股骨头缺血性坏死,髋膝关节功能恢复良好.结论 采用带缝匠肌蒂髂骨瓣移植加空心加压螺纹钉内固定治疗青壮年股骨颈骨折可促进骨折愈合,降低股骨头缺血坏死的发生率,是一种治疗青壮年股骨颈骨折的较好方法.     

带股方肌肌蒂骨瓣治疗青壮年股骨颈骨折

目的 评价带股方肌肌蒂骨瓣治疗青壮年股骨颈骨折的效果。方法 本组32例患者均为Garden3-4型股骨颈骨折,术前证实有股骨头血液回流障碍。全部采用手术切开复位,加压螺纹钉内固定联合带股方肌肌蒂骨瓣移植术,术后制动12周。平均随访时间3年9个月。结果 股骨颈骨折全部愈合,时间6－9个月。3例发生股骨头坏死,1例于术后6个月在X线片上出现股骨头坏死像,1例于术后9个月出现坏死像,1例术后发生感染,经清创后,于术后6个月出现股骨头坏死的X线表现。其中前2例为Garden4型,术前检查,股骨头部完全无静脉回流。结论 带股方肌肌蒂骨瓣移植加压螺纹钉内固定术治疗青壮年股骨颈肌骨折,具有血供丰富,创伤小及减少股骨头坏死发生率的良好效果。     

带血管蒂肌骨瓣结合空心加压螺纹钉治疗头下型股骨颈骨折41例分析

头下型股骨颈骨折，尤其是损伤较严重的GardenⅢ、Ⅳ型骨折较容易发生股骨头缺血性坏死及骨不连．本院从1994年3月～2002年3月采用带股方肌血管蒂肌骨瓣或带缝匠肌血管蒂肌骨瓣植骨加3枚空心加压钉内固定．术后配合使用解痉、扩血管等药物治疗41例GardenⅢ、Ⅳ型头下型股骨颈骨折并取得了良好的效果．较大降低了股骨头缺血坏死及骨不连率。     

空心加压螺纹钉经皮内固定治疗股骨颈骨折

目的 探讨空心加压螺纹钉经皮内固定治疗股骨颈骨折的临床疗效.方法 对55例股骨颈骨折Garden Ⅰ、Ⅱ型21例,GardenⅢ、Ⅳ型34例采用空心加压螺纹钉经皮内固定.结果 术中出血量<20ml,手术时间20～55min,平均31min.55例随访18～42个月,平均27.6个月,96.4%(53/55)骨折愈合,愈合时间3～13个月,平均6.1个月;髋关节功能Harris评分:优37例,良12例,可4例,差2例,优良率89.1%(49/55).21例Garden Ⅰ、Ⅱ型中,无骨折不愈合及股骨头缺血坏死;34例Gar-denⅢ、Ⅳ型中,骨折不愈合率及股骨头缺血坏死率分别5.9%(2/34)和11.7%(4/34).结论 空心加压螺纹钉经皮内固定治疗股骨颈骨折疗效理想,具有操作简便、创伤小、手术时间短等优点.     

股方肌骨瓣转移空心加压钉治疗青壮年股骨颈GardenⅢ、Ⅳ型骨折

目的：探讨股方肌骨瓣移植空心加压钉治疗青壮年股骨颈GardenⅢ、Ⅳ型骨折的疗效。方法对52例青壮年股骨颈GardenⅢ、Ⅳ型骨折患者采用股方肌骨瓣移植空心加压钉治疗,观察其治疗效果。结果本组获随访6个月～5年,2例未愈合,1例畸形愈合。6例股骨头坏死。根据Harris髋关节功能评分标准,优良率84.6%。结论股方肌骨瓣移植空心加压螺钉治疗青壮年股骨颈GardenⅢ、Ⅳ型骨折疗效可靠,手术操作简单,髋关节功能恢复好。     

空心双头螺纹钉加压固定骨瓣移植治疗青壮年股骨颈骨折

目的:探讨青壮年股骨颈骨折的治疗。方法:采用切开复位空心双头螺纹钉加压内固定缝匠肌骨瓣移植治疗青壮年股骨颈骨折,随访2~8年,平均4年。结果:43例新鲜骨折中1例不愈合股骨头坏死,1例骨折愈合后股骨头坏死;27例陈旧性骨折中,3例骨折不愈合股骨头坏死,2例愈合后股骨头坏死。愈合率94%,股骨头坏死率10%。结论:缝匠肌骨瓣移植可改善股骨头血供并使植骨以"活骨骨折愈合"的方式完成。陈旧性骨折股骨颈吸收可重建股骨颈,与空心双头螺纹加压钉有协同支撑固定的作用,是治疗青壮年股骨颈骨折的有效方法。     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Expression of OPGmRNA and ODFmRNA in the patients of hip fracture due to osteoporosis

Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.     

The clinicopathological and immuohistochemical analysis of solid-pseudopapillary tumor of the pancreas： report of 9 cases

Objective：To investigate the clinical features, pathological characteristics and immunophenotype of solid-pseudopapillary tumor of the pancreas（SPTP）. Methods：Nine surgically treated cases of SPTP were retrospectively reviewed. Hematoxylin and Eosin（HE） staining and immunohistochemical staining were used to analyze all cases, and the general clinical data was collected. Results：Six patients were asymptomatic except for a palpable mass. Two patients complained of vague-epigastric pain. One patient appeared jaundice. The tumor was encapsulated and solid tissues alternately with cystic tissues. Histologically, the histological structure of solid portion was pseudopapillary with a fibrovascular core. Tumor cells were uniform and medium-sized which were arranged in sheets ets or nests or pseudopapillary patterns. Immunohistochemical studies demonstrated that SPTP proved positive in vimentin（9/9 cases）, AAT（9/9 cases）, NSE（9/9 cases）, ACT（7/9 cases）, CK20（2/9 cases）, CgA（1/9 cases）, S-100（3/gcases）, PR（4/gcases）, Syn（3/9 cases） and CD56（5/9cases）, negative in CEA and ER. Conclusion：SPTP is a tumor predominantly occurring in young women frequently without special symptoms. This tumor has various characteristical histological patterns with different immunophenotype.     

Dynamic Changes in Serum Estradiol and Progesterone levels in Patients of Premenstrual Syndrome with Adverse Flow of Liver-qi

Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.     

Real-time Three-dimensional Echocardiography in Assessment of Left Ventricular and Right Ventricular Volumes

Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.     

SCREENING FOR A 21-CHROMOSOME ABNORMALITY IN PREIMPLANTED EMBRYOS OF ELDERLY WOMEN

Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.