微针介导HGF基因表达治疗湿疹的实验研究

目的 通过微针经皮递送携带肝细胞生长因子(HGF)重组质粒pHGF,探究微针介导HGF基因表达治疗湿疹的作用.方法 制备筛选pHGF透明质酸可溶性微针贴并进行表观机械性能检测、皮肤插入染色试验及小动物活体成像.实验小鼠随机分为模型组、阳性对照组、微针贴组及皮下注射组,采用2,4-二硝基氯苯(DNCB)皮肤致敏建立湿疹模型,按皮肤症状评分和苏木素-伊红(HE)染色切片观察皮肤病理情况,评价载药微针贴的治疗效果.结果 透明质酸可溶性微针贴结构完整,机械强度较好,能较好地插入小鼠皮肤成功染色并能将质粒DNA有效递送到皮内表达.第28天后与模型组BALB/c小鼠相比,微针贴组和皮下注射组皮损积分较低(均P＜0.01),HE病理染色切片显示,皮肤炎性浸润较轻,皮下附件修复较好.结论 微针经皮递送pHGF基因表达能对湿疹模型小鼠起到较好的治疗作用.     

湿润烧伤膏对寻常型银屑病小鼠IL23/Th17轴及其相关因子表达水平的影响

目的观察湿润烧伤膏(moist exposed burn ointment,MEBO)对咪喹莫特诱导的寻常型银屑病小鼠皮损组织中白细胞介素-17 (IL-17)、白细胞介素-23 (IL-23)、肿瘤坏死因子-α(TNF-α)及干扰素-γ(IFN-γ)水平的影响,并探讨其作用机制。方法选取40只SPF级健康雌性6～7周龄近交系BALB/c小鼠建立咪喹莫特诱导的寻常型银屑病模型,模型建立成功后采用随机数表法将35只小鼠随机分为对照组、模型组、MEBO低剂量组、MEBO中剂量组及MEBO高剂量组,并分别采用卡泊三醇乳膏、麻油、1:3湿润烧伤膏与麻油混合物、1:1湿润烧伤膏与麻油混合物、湿润烧伤膏处理皮损创面,治疗第7、14天参照银屑病皮损面积与严重程度指数(psoriasis area and severity index,PASI)评分标准评估5组小鼠皮损严重程度,治疗第14、28天采用Western blotting法检测IL-17、IL-23、TNF-α及IFN-γ蛋白表达水平,并予以对比。结果治疗第7天,对照组、MEBO中剂量组及MEBO高剂量组小鼠背部皮损PASI评分均显著低于模型组和MEBO低剂量组(P均0.05),且对照组、MEBO中剂量组及MEBO高剂量组组间两两对比,PASI评分均无明显差异(P均0.05);治疗第14天,5组小鼠皮损症状均明显改善,PASI评分无明显差异(P0.05);治疗第14天,小鼠背部皮损组织中IL-17、IL-23、TNF-α及IFN-γ蛋白表达水平对比,对照组显著低于其他各组(P均0.05),且MEBO高剂量组显著低于模型组(P均0.05);治疗第28天,小鼠背部皮损组织中IL-17、IL-23、TNF-α及IFN-γ蛋白表达水平对比,对照组显著低于模型组、MEBO低剂量组及MEBO中剂量组(P均0.05),与MEBO高剂量组无明显差异(P均0.05),MEBO高剂量组和MEBO中剂量组显著低于模型组和MEBO低剂量组(P均0.05),且MEBO高剂量组与MEBO中剂量组无明显差异(P均0.05)。结论湿润烧伤膏治疗银屑病的疗效与卡泊三醇软膏相当,且调节IL-23/Th17轴及其相关因子的表达水平,抑制炎症反应可能是其作用机制。     

Sirolimus therapy for a cGVIID murine model with scleroderma by up-regulating Tregs

目的 基于建立的表现为皮肤胶原沉着的小鼠慢性移植物抗宿主病模型,探讨西罗莫司对该慢性排斥反应的治疗作用及其作用机制.方法 建立B10.D2→BALB/c异基因移植小鼠模型,随机分为两组,异基因移植治疗组(实验组)口服西罗莫司,连续治疗,剂量3 mg/(kg·d);异基因移植对照组(对照组)口服橄榄油.同时移植BALB/c供体小鼠细胞混悬液给受体BALB/c小鼠作为同基因移植组.记录小鼠体质量和状态变化情况;取背部皮肤,进行病理分析;提取RNA,实时荧光定量PCR检测趋化因子(RANTES、MCP-I)及纤维生成相关因子(TGF-β1、胶原I)表达水平的变化;取外周血和脾细胞,通过流式细胞仪分析CD4+CD25+FOXP3+Treg细胞水平的变化.结果 观察期结束(11周)时,西罗莫司治疗组小鼠体质量[(20.43±2.27)g]与橄榄油对照组[(14.13±1.47)g]相比明显恢复(P相似文献   

自配外用中药治疗小腿慢性湿疹38例

湿疹是一种常见的过敏性炎性皮肤病。慢性湿疹多由急性湿疹反复发作而致。由于病因不确切 ,单纯采用西医治疗往往疗效不佳 ,而且容易反复。1995～ 2 0 0 0年 ,我们自配中药外用水剂和膏剂辅以口服西药治疗小腿慢性湿疹 38例 ,效果显著。1　临床资料1 1　一般情况　 38例中 ,男 2 2例 ,女 16例 ;年龄 5～ 60岁。病程 3个月～ 3年。大多以双侧小腿伸侧皮肤发病 ,以局部皮肤瘙痒、皮损呈多样性为特点。早期局部皮损以红斑、丘疹、水疱为主 ,因瘙痒抓破后出现糜烂、渗液 ,继而出现结痂和脱屑。病情反复发作 ,久治不愈而进入慢性期 ,进而皮肤变厚…     

芥菜籽对BALB/c小鼠变应性接触性皮炎的作用研究

目的探讨食用5%芥菜籽饲料对小鼠变应性接触性皮炎模型的作用及机制。方法将30只SPF级雌性BALB/c小鼠随机分为芥菜籽组、模型对照组、正常对照组。模型对照组和正常对照组给予普通饲料,芥菜籽组给予含5%芥菜籽饲料。喂养3周后,用2,4-二硝基氟苯诱导小鼠ACD模型:实验第1、2天用0.5%2,4-二硝基氟苯溶液外涂于背部剃毛处,第6天用0.25%2,4-二硝基氟苯溶液外涂左耳背腹面进行激发。耳部激发24 h后,取小鼠耳片测量质量差,行HE染色观察局部炎症细胞浸润情况,采用ELISA方法检测血清TNF-α的变化。结果芥菜籽组小鼠耳片质量的改变明显小于模型对照组,局部炎症细胞计数明显减少,血清TNF-α值亦低于模型对照组(P<0.05)。结论喂饲5%芥菜籽饲料可有效抑制2,4-二硝基氟苯所诱导的小鼠ACD,显著减轻水肿和炎症细胞浸润,下调血清TNF-α。     

芥菜籽对BALB/c小鼠变应性接触性皮炎的作用研究

目的探讨食用5%芥菜籽饲料对小鼠变应性接触性皮炎模型的作用及机制。方法将30只SPF级雌性BALB/c小鼠随机分为芥菜籽组、模型对照组、正常对照组。模型对照组和正常对照组给予普通饲料,芥菜籽组给予含5%芥菜籽饲料。喂养3周后,用2,4-二硝基氟苯诱导小鼠ACD模型：实验第1、2天用0.5%2,4-二硝基氟苯溶液外涂于背部剃毛处,第6天用0.25%2,4-二硝基氟苯溶液外涂左耳背腹面进行激发。耳部激发24 h后,取小鼠耳片测量质量差,行HE染色观察局部炎症细胞浸润情况,采用ELISA方法检测血清TNF-α的变化。结果芥菜籽组小鼠耳片质量的改变明显小于模型对照组,局部炎症细胞计数明显减少,血清TNF-α值亦低于模型对照组（P〈0.05）。结论喂饲5%芥菜籽饲料可有效抑制2,4-二硝基氟苯所诱导的小鼠ACD,显著减轻水肿和炎症细胞浸润,下调血清TNF-α。     

西罗莫司对小鼠皮肤胶原沉着病模型的治疗作用及可能作用机制的探讨

目的基于建立的表现为皮肤胶原沉着的小鼠慢性移植物抗宿主病模型,探讨西罗莫司对该慢性排斥反应的治疗作用及其作用机制。方法建立B10.D2→BALB/c异基因移植小鼠模型,随机分为两组,异基因移植治疗组(实验组)口服西罗莫司,连续治疗,剂量3 mg/(kg.d);异基因移植对照组(对照组)口服橄榄油。同时移植BALB/c供体小鼠细胞混悬液给受体BALB/c小鼠作为同基因移植组。记录小鼠体质量和状态变化情况;取背部皮肤,进行病理分析;提取RNA,实时荧光定量PCR检测趋化因子(RANTES、MCP-1)及纤维生成相关因子(TGF-β1、胶原Ⅰ)表达水平的变化;取外周血和脾细胞,通过流式细胞仪分析CD4+CD25+FOXP3+Treg细胞水平的变化。结果观察期结束(11周)时,西罗莫司治疗组小鼠体质量[(20.43±2.27)g]与橄榄油对照组[(14.13±1.47)g]相比明显恢复(P<0.05);病理分析显示,与橄榄油对照组相比,西罗莫司治疗组小鼠皮肤无明显的增厚变硬,纤维增生以及炎症细胞浸润;实时荧光定量PCR检测证实,西罗莫司治疗组小鼠皮肤RANTES、胶原Ⅰ、TGF-β1以及MCP-1 mRNA转录水平较橄榄油对照组显著下调(P<0.05)。此外,流式细胞仪检测结果显示,西罗莫司治疗组小鼠外周血中CD4+CD25+FOXP3+Treg细胞所占比例[(11.47±1.42)%]与橄榄油对照组[(7.57±0.63)%]相比显著上调(P<0.05);西罗莫司治疗组小鼠脾细胞中CD4+CD25+FOXP3+Treg细胞所占比例[(24.55±0.21)%]与橄榄油对照组[(11.05±1.2)%]相比显著上调(P<0.01)。结论西罗莫司可能通过上调CD4+CD25+FOXP3+Treg细胞,抑制效应细胞的活化,下调炎症细胞以及趋化因子的分泌表达,从而改善小鼠皮肤病变(胶原沉着以及炎性浸润),显著提高小鼠的生存质量。     

十二烷基硫酸钠诱导银屑病动物模型的建立

目的建立一种简单、快速筛选银屑病治疗药物的模型。方法应用10%十二烷基硫酸钠(sodium dodecyl sulfate,SDS)涂抹模型组小鼠背部皮肤,对照组小鼠涂抹蒸馏水,观察两组皮肤组织的改变。结果经过10% SDS涂抹7 d后的模型组小鼠皮肤可见明显银屑病样体征,如皮肤增厚[(0.87±0.33)mm vs(0.60±0.02)mm]、红肿和皮下血管增生等。病理检测证明模型组小鼠皮肤出现角化过度和角质层增厚,真皮层和皮下层可见毛细血管扩张和充血等银屑病样改变。West-ern-blot结果证明,血清中的PKCβ、IL-1β、TNF-α、IL-23等细胞因子表达上调。结果显示SDS所诱导的小鼠模型能够反映银屑病的某些特征。结论利用10%SDS涂抹小鼠皮肤可建立银屑病动物模型,方法简单、快速。     

湿疹乳膏的药效学研究

目的:考察湿疹乳膏的药效作用。方法:观察湿疹乳膏高、中、低剂量对二甲苯所致小鼠耳肿胀、DNCB诱发豚鼠过敏性接触性皮炎的作用和对磷酸组胺致痒阈的影响以及对常见病原菌的体外抑菌情况。结果:湿疹乳膏高、中、低剂量对二甲苯所致小鼠耳肿胀有显著的抑制作用,对DNCB诱发豚鼠过敏性接触性皮炎有非常显著的抑制作用,能显著提高磷酸组胺致痒阈,对常见病原菌有一定的抑制作用。结论:湿疹乳膏具有抗炎、止痒及抗菌的功效。     

自制湿疹膏治疗小儿湿疹70例

自1993年6月以来，应用自制的湿疹膏治疗小儿湿疹70例，取得满意疗效。临床资料一、一般情况本组男34例，女36例；均为门诊病人。年龄14d～11岁，其中14d～1岁占44例。病程3d～11年。皮损部位以面部、四肢为多，其次为外耳道、阴囊、全身皮肤等。皮肤症状以亚急性居多，其次为慢性湿疹。全部病例严格按湿疹诊断标准确诊。二、制剂湿疹膏主要由持美肤[2]0．1g加基质至100g配制成。并用KM小鼠皮肤斑贴实验方法，观察该药对小鼠皮肤的刺激性，未见明显不良反应。三、治疗方法每日在患处落涂湿疹膏2次，7～10d为1个疗程，如果皮报有渗出，可先…     

Effect of corticosteroids on 18F-FDG uptake in tumor lesions after chemotherapy.

To be a reliable predictor of response, (18)F-FDG uptake should reflect changes in the amount of viable tumor cells. However, (18)F-FDG also accumulates in inflammatory cells. Shortly after treatment, the influx of inflammatory cells in the tumor can therefore interfere with early response evaluation. The aim of this study was to investigate whether this inflammation is suppressed by the administration of corticosteroids and, in turn, can improve the correlation of (18)F-FDG uptake with tumor cell kill. METHODS: Severe combined immunodeficiency mice were inoculated subcutaneously with Daudi cells. When the tumor measured 15 mm, mice were divided in 2 groups treated with 1 single dose of cyclophosphamide, 125 mg/kg (group A) or cyclophosphamide followed by hydrocortisone (0.2 mg/d) for 5 d (group B). The change in (18)F-FDG uptake was evaluated with small-animal PET (5 mice/group) on D+6, D+9, D+13, and D+16 (days after treatment). At each time point, 4 mice per group were sacrificed for quantification of the different tumor cell fractions by flow cytometry and histopathology. Changes in (18)F-FDG uptake were correlated with inflammation and viable tumor cells. RESULTS: Cyclophosphamide administration resulted in a steady reduction in viable cell fraction until D+9 (reduction from baseline, -64%). The viable cell fraction increased again on D+13. A transient influx of inflammatory cells was seen from D+6 to D+13 (peak on D+9, 24% of total cell fraction). After hydrocortisone administration, a similar reduction in the viable cell fraction was seen. The inflammatory response was less pronounced but developed with earlier kinetics (peak on D+6 [15% of total cell fraction], almost resolved on D+9) and consisted primarily of granulocytes instead of mononuclear cells in the absence of corticosteroids. In both groups, a significant reduction in (18)F-FDG uptake was seen until D+6. On D+9, a transient increase in (18)F-FDG uptake was seen in group A, whereas a further decrease was observed in group B. CONCLUSION: After corticosteroid administration, the contribution of inflammatory cells to the (18)F-FDG uptake was less important than that in mice treated with chemotherapy alone. The earlier, but weaker, inflammatory response after corticosteroid administration consists primarily of granulocytes instead of mononuclear cells.     

胸部增强CT扫描鉴别诊断肺内孤立性结节的研究

目的探讨增强CT扫描鉴别诊断肺内孤立性结节的意义以及强化程度与富血管度的关系。方法病理证实的肺癌11例、炎性结节13例、结核瘤6例,术前行CT平扫和以2.5ml/s速率注入碘造影剂100ml后增强扫描,观察病灶强化程度及强化形态,并计算以上30例病灶的手术切除标本的血管数目,与术前增强CT扫描强化程度做相关分析。结果恶性肿瘤的强化程度明显高于结核(P<0.01)。两者的强化形态也不同。恶性肿瘤与炎症强化程度无显著差异(P>0.05),两者强化形态亦无明显不同。观察手术切除标本,病灶强化程度与富血管度有相关性。结论CT平扫不能鉴别肺内孤立性结节的性质。增强CT扫描肿瘤与结核的强化程度及强化形态均不同,可以进行鉴别。但恶性肿瘤与炎性结节强化程度无显著差异,强化形态也相似,故不易鉴别。病灶强化程度反映其富血管度。     

Production of TNF-alpha by skin explants of dinitrochlorobenzene-challenged ears in rats: a model for the evaluation of contact hypersensitivity

BACKGROUND: Contact hypersensitivity (CHS) is a local inflammatory response of the skin following challenge of hapten-sensitized animals. It is the consequence of cell infiltration of derm and the release of inflammation mediators, among which Tumor necrosis factor-alpha (TNF-alpha) is one of the most important factors. The intensity of the inflammation could be quantified by ear swelling which is the classical manifestation of the reaction. This study was testing the working hypothesis that levels of TNF-alpha in skin organ culture medium should correlate with the intensity of CHS reaction measured in vivo by ear swelling assay, and with the density of dermal infiltrate in ear skin samples. In order to test the working hypothesis, the intensity of inflammatory reaction following challenge was evaluated by classical measurements of ear swelling, by the determination of TNF-alpha levels in culture fluids of ear skin following epicutaneous application of dinitrochlorobenzene (DNCB) into the ears of sensitized animals. METHODS: Animal model of CHS reaction to DNCB in Albino Oxford rats was used as described. Ear swelling was quantified in percentage terms as the difference in thickness between the challenged and nontreated ears of the same animal. Dermal infiltrate density in histopathologically analyzed samples of ear skin was evaluated by computer-assisted image analysis. Ear skin samples were cultured in standard medium for 24 h, and TNF-alpha concentration in the conditioned medium was subsequently determined with ELISA test. RESULTS: Dose-dependent increase in the density of the dermal infiltrate and in TNF-alpha in CM were noted following the application of 0.65%, 1.3% and 2.6% of DNCB to the ears of previously sensitized rats. The correlation between ear swelling and the levels of TNF-alpha (r = 0.933, p < 0.001) in CM, and between ear swelling and dermal infiltrate density (r = 0.916, p < 0.001) was found. Correlation was also found between the density of the dermal infiltrate and the levels of TNF-alpha (r = 0.865, p < 0.001). CONCLUSION: Presented data suggested that skin-organ culture system and the quantification of inflammatory mediators might be used for the evaluation of contact hypersensitivity reaction and its intensity.     

A rabbit model of atherosclerosis at carotid artery: MRI visualization and histopathological characterization

To induce a rabbit model of atherosclerosis at carotid artery, to visualize the lesion evolution with magnetic resonance imaging (MRI), and to characterize the lesion types by histopathology. Atherosclerosis at the right common carotid artery (RCCA) was induced in 23 rabbits by high-lipid diet following balloon catheter injury to the endothelium. The rabbits were examined in vivo with a 1.5-T MRI and randomly divided into three groups of 6 weeks (n=6), 12 weeks (n=8) and 15 weeks (n=9) for postmortem histopathology. The lesions on both MRI and histology were categorized according to the American Heart Association (AHA) classifications of atherosclerosis. Type I and type II of atherosclerotic changes were detected at week 6, i.e., nearly normal signal intensity (SI) of the injured RCCA wall without stenosis on MRI, but with subendothelial inflammatory infiltration and proliferation of smooth muscle cells on histopathology. At week 12, 75.0% and 62.5% of type III changes were encountered on MRI and histopathology respectively with thicker injured RCCA wall of increased SI on T(1)-weighted and proton density (PD)-weighted MRI and microscopically a higher degree of plaque formation. At week 15, carotid atherosclerosis became more advanced, i.e., type IV and type V in 55.6% and 22.2% of the lesions with MRI and 55.6% and 33.3% of the lesions with histopathology, respectively. Statistical analysis revealed a significant agreement (p<0.05) between the MRI and histological findings for lesion classification (r=0.96). A rabbit model of carotid artery atherosclerosis has been successfully induced and noninvasively visualized. The atherosclerotic plaque formation evolved from type I to type V with time, which could be monitored with 1.5-T MRI and confirmed with histomorphology. This experimental setting can be applied in preclinical research on atherosclerosis.     

An experimental study on differential diagnosis of tumor from inflammation by using 125I labeled Pisum sativum agglutinin

We have reported that Pisum sativum agglutinin (PSA), a plant lectin which recognizes mannosyl residues, accumulates markedly in Ehrlich solid tumor (EST) and suggested the possibility of applying PSA to tumor imaging radiopharmaceuticals. In the present work, an inflammation was induced by implantation of cotton thread in the left rear leg skeletal muscle of ddY mice and Ehrlich ascites tumor cells were inoculated into the right rear leg. ⁶⁷Gacitrate accumulated in the tumor tissue and the inflammatory lesion to almost equal extents. On the other hand, ¹²⁵IPSA preferentially accumulated in tumor tissues in mice bearing both tumor and inflammation. The results suggest that differential diagnosis of tumor from inflammation using radiolabeled PSA may be possible.     

Intraductal papillary tumors of the pancreas. Histopathologic correlation of MR cholangiopancreatography findings

Purpose: To evaluate MR cholangiopancreatography (MRCP) findings of intraductal papillary tumors of the pancreas and correlate them with histopathology.Material and Methods: Seventeen patients with intraductal papillary tumor of the pancreas underwent MRCP before surgery. MRCP findings were correlated to histopathology with regard to the presence of septa and excrescent nodules in the cystic lesion, communication between the cystic lesion and the main pancreatic duct (MPD), degree of dilatation of MPD, and dilatation of the common bile duct (CBD).Results: MRCP demonstrated septa in 17 cases (100%), excrescent nodules in 8 cases (47.1%), communication between the intraductal papillary tumor and the MPD in 14 cases (82.3%), dilatation of MPD over 50% in 6 cases (35.3%), and dilatation of CBD in 3 cases (17.6%). These findings showed excellent correlation with histopathology. The septum on MRCP corresponded with a layer of connective tissue with pancreatic duct epithelium. Excrescent nodules in the carcinomas consisted not only of malignant cells, but also of dysplasia and adenoma. Excrescent nodules in adenomas were consistent not only with minimal papillary growth of adenoma, but also with proliferation of fibrosis, and hematoma and organized fibrin with minimal fibrosis. Pancreatic tissue was affected by chronic pancreatitis in all cases. Cases with dilatation of CBD on MRCP were due to microscopic invasion by the carcinoma.Conclusion: MRCP appearances of intraductal papillary tumors are well correlated with the findings at histopathology.     

MR imaging in an experimental model of brain tumor immunotherapy

A murine model of implanted CNS neoplasia was used to study a new form of brain tumor immunotherapy with intralesional Corynebacterium parvum (C. parvum). Assessment of treatment protocols has been limited by the inability to assess, noninvasively, tumor burden and/or the inflammatory reaction induced in the murine brain by treatment with C. parvum. This study demonstrates that contrast-enhanced MR imaging can monitor in vivo tumor burden and the immune response to intracerebral C. parvum. KHT murine sarcoma was stereotaxically implanted into the right frontal lobe of C3H/HeN mice at doses of 10,000 and 50,000 tumor cells. The KHT sarcoma is 100% fatal in untreated mice. Therapy consisted of an intraperitoneal injection of 350 micrograms of killed C. parvum 1 day after tumor implantation followed by 70 micrograms of C. parvum stereotaxically injected into the tumor 5 days after implantation. MR imaging was performed on mice injected with saline only, C parvum only, tumor only, and tumor treated with C. parvum. C. parvum alone elicited an intense transitory mononuclear cell inflammatory reaction in the meninges, ependyma, and to a variable degree at the injection site. The inflammatory response reached a peak 2 weeks after intracerebral injection. Contrast-enhanced MR imaging was able to detect the presence and severity of C. parvum-induced inflammation, which decreased 3 weeks after intracerebral injection. The transitory nature of this type of inflammation should allow its differentiation from tumor in subjects undergoing serial scanning following intracerebral injection of C. parvum as a form of brain tumor immunotherapy.     

Postinflammatory hyperpigmentation following torture

Hyperpigmentation after torture in darker skinned patients has regularly been noted, although its pathophysiology, and thus its forensic importance, has not previously been documented. Hyperpigmentation is not well described in the dermatological literature. It is the result of inflammation. Any inflammation can cause hyperpigmentation, and the shape of the resulting lesion can closely follow the contours of the site of original inflammatory response. This can be important in correlating the lesion with the alleged cause. It also helps to establish the differential diagnosis of the lesion, which also assists in assessing the degree of consistency between the lesion and the alleged cause. Patterns of hyperpigmentation can therefore, be helpful in assessing allegations of torture months or years after the event.     

依达拉奉对实验性小鼠自身免疫性脑脊髓炎的抑制作用

目的：研究依达拉奉对实验性自身免疫性脑脊髓炎（experimental autoimmune encephalomyelitis , EAE）小鼠的抑制作用。方法将60只雌性C57BL/6小鼠随机分为3组：正常对照组、EAE组、EAE＋依达拉奉组,每组20只。正常对照组用PBS作为对照。后两组应用髓鞘少突胶质细胞糖蛋白35-55、完全福氏佐剂（ CFA）、结核分枝杆菌,制成的抗原乳剂进行造模。免疫2 d后,EAE＋依达拉奉组腹腔注射依达拉奉5 mg/（ kg· d）。3组均监测20 d,每日称重,观察小鼠的摄食和发病情况,并进行神经功能评分。20 d后取小鼠脊髓组织,进行病理学观察。结果 EAE组小鼠的发病率（80％）高于EAE＋依达拉奉组（45％）,差异有统计学意义（P＜0．05）。 EAE＋依达拉奉组神经功能评分各个时间点都低于EAE组,差异有统计学意义（P＜0．05）,其中,监测10 d后,EAE组神经功能评分（3．6±0．4）级;而EAE＋依达拉奉组评分为（1．3±0．5）级。 HE染色后发现,EAE组小鼠的脊髓内有大量的炎性细胞浸润,白质脱髓鞘改变明显。而EAE＋依达拉奉组小鼠有较少的炎性细胞浸润,且没有白质脱髓鞘改变。结论依达拉奉对EAE的保护作用可能与其清除自由基、减轻炎性反应有关。     

An autopsy case of otogenic intracranial abscess and meningitis with Bezold’s abscess: Evaluation of inflammatory bone destruction by postmortem cone-beam CT

The deceased was an unidentified young male found unconscious on a walkway. On autopsy, outer and inner fistulae of the left temporal bone, subcutaneous abscess in the left side of the neck and head, and an intracranial abscess were noted. A portion of the left temporal bone was removed and scanned by cone-beam computed tomography (CT) (normally used for dentistry applications) to evaluate the lesion. The cone-beam CT image revealed roughening of the bone wall and hypolucency of the mastoid air cells, consistent with an inflammatory bone lesion. According to autopsy and imaging findings, the cause of death was diagnosed as intracranial abscess with Bezold’s abscess secondary to left mastoiditis as a complication of otitis media. Although determining the histopathology of bone specimens is time-consuming and costly work, we believe that use of cone-beam CT for hard tissue specimens can be useful in forensic practice.