锥体束受侵犯程度与岛叶胶质瘤患者术后疗效的关系

目的探讨锥体束受侵犯程度与岛叶胶质瘤患者术后疗效的关系。方法回顾性分析2010年7月至2019年7月于中国科学技术大学附属第一医院(安徽省立医院)神经外科行手术治疗的41例岛叶胶质瘤患者的临床资料。根据MRI显示锥体束受侵犯程度将患者分为3型,其中肿瘤仅侵犯岛叶前下部为Ⅰ型,侵犯岛叶后上部为Ⅱ型,浸润破坏内囊结构为Ⅲ型。分析各型患者术前、术后肢体运动功能障碍,病理学类型及术后疗效的差异。结果41例患者中,Ⅰ型18例(43.9%),Ⅱ型20例(48.8%),Ⅲ型3例(7.3%)。Ⅰ型患者术前、术后均无运动功能障碍。Ⅱ型患者术前4例有运动功能障碍,其中3例术后得到了改善,1例症状进一步加重,1例术后新发生了运动功能障碍。Ⅲ型患者术前均有运动功能障碍,术后症状均进一步加重。Ⅰ、Ⅱ、Ⅲ型肿瘤全切除者分别有11例、5例、1例,与Ⅰ型比较,Ⅱ+Ⅲ型肿瘤全切除比例低[分别为26.1%(6/23),11/18],差异有统计学意义(P=0.026);Ⅰ、Ⅱ、Ⅲ型患者中,世界卫生组织(WHO)Ⅲ~Ⅳ级胶质瘤患者分别有3例(3/18)、7例(7/20)、3例(3/3),差异有统计学意义(P=0.015)。术后29例获3~72(22.7±12.0)个月的随访。Ⅰ型患者随访13例,均未复发;Ⅱ型患者随访15例,其中7例复发;Ⅲ型患者随访1例,术后第3个月因残留肿瘤增大而死亡。Kaplan-Meier生存分析3型患者的生存率差异有统计学意义(P<0.01)。结论锥体束受侵犯程度越重,岛叶胶质瘤的病理学级别越高,手术全切除率越低,术后疗效越差。     

Abnormalities in diffusion tensor imaging of the uncinate fasciculus relate to reduced memory in temporal lobe epilepsy

Aims: To use Diffusion Tensor Imaging (DTI) to explore structural integrity and connectivity of the uncinate fasciculus (UF) in patients with temporal lobe epilepsy (TLE) and its relationship to memory performance.
Methods: DTI and UF reconstruction were performed in 28 patients with TLE (18 left, 10 right) and 10 normal controls. Differences between left and right UF fractional anisotropy (FA) and apparent diffusion coefficient (ADC) values and correlations between DTI measures and memory scores in the TLE groups were computed.
Results: In controls, FA was higher in the left than right UF (p < 0.01). In left TLE, FA values were lower and ADC values higher than controls in the left UF and ADC values were higher in the right UF (all p < 0.05). In right TLE, ADCs were higher in the left and right UF compared to controls, and FA was reduced in the left UF (all p < 0.05). In left TLE, ADCs in the left UF were negatively correlated with Auditory Immediate (p < 0.05) and Delayed Memory (p < 0.01). Visual Delayed Memory was positively correlated with reduced FA in the ROI of the right UF and increased radial diffusivities (p < 0.05). No significant correlations were found in right TLE. Thus, DTI values correlated with memory scores in the expected direction in patients with left TLE.
Conclusions: Abnormal diffusion measures in the UF ipsilateral to the epileptogenic zone suggest that integrity of the UF is related to memory performance in patients with left TLE. Larger sample sizes are needed to evaluate structure-function correlations further.     

Awake craniotomy versus craniotomy under general anesthesia for the surgical treatment of insular glioma: choices and outcomes

Objective: To investigate differences in outcomes in patients who underwent surgery for insular glioma using an awake craniotomy (AC) vs. a craniotomy under general anesthesia (GA).

Methods: Data from patients treated at our hospital between 2005 and 2015 were analyzed retrospectively. The preoperative, intraoperative, postoperative, and longer term follow-up characteristics and outcomes of patients who underwent surgery for primary insular glioma using either an AC or GA were compared.

Results: Of the 52 identified patients, 24 had surgery using an AC and 28 had surgery under GA. The extent of resection was similar for the two anesthesia techniques: the median extent of resection was 61.4% (IQR: 37.8–74.3%) in the WHO grade <4 AC group vs. 50.5% (IQR: 35.0–71.2%) in the grade <4 GA group and 73.4% (IQR: 54.8–87.2%) in the grade 4 AC group vs. 88.6% (IQR: 61.2–93.0%) in the grade 4 GA group. Consistent with literature, there were more early neurological deficits after an AC, while the GA group showed more new late neurological deficits; however, these trends were not significant. Survival was similar between the two groups, with 100% 1- and 2-year survival in the grade <4 groups.

Conclusion: Our results showed that the extent of resection, neurological outcomes, and survival were similar using the two anesthesia techniques. Since AC is more challenging for the patient and for his or her caregiver after surgery, this finding has implications for clinical decision-making.     

免疫磁珠法分离、培养人脑胶质瘤干细胞

目的建立免疫磁珠法分离，并培养人脑胶质瘤干细胞的方法。方法将术中取得的脑胶质瘤标本，通过剪切、消化和吹打成单细胞悬液，筛网过滤，免疫磁珠分选试剂盒分选出CD133^＋细胞，用神经干细胞无血清培养法培养出具有单细胞克隆能力的细胞球，取第3代进行诱导分化，分化前后用免疫细胞荧光化学方法鉴定肿瘤干细胞及分化后细胞。结果免疫磁珠分选出的CD133^＋细胞，可悬浮生长并形成神经干细胞样细胞球，有较强的增殖能力，干细胞标志物巢蛋白（nestin）阳性，分化后细胞表达神经元小管相关蛋白β-3（β-tubulin3）和星形胶质细胞胶质纤维酸性蛋白质（GFAP）特异性抗原，而巢蛋白、CD133^＋阴性，并具有肿瘤的核型。结论免疫磁珠分选法可避免原代培养中众多细胞混杂生长的发生，能够从大量肿瘤细胞中分离出只占极少比例的肿瘤干细胞，细胞结合磁珠后在体外可以长期培养和传代，进一步证实了肿瘤干细胞的存在，并为胶质瘤干细胞的研究奠定基础。     

Cystic glioma of the cerebral hemispheres,with long survival

Summary Seven cases of cystic benign glioma of the cerebral hemispheres are presented. The histological characteristics of this type of tumor are described, stressing the need to consider it as an independent entity in the gliomas' classification. The prognosis is exceptionally good in terms of long survival and minimal residua after surgical treatment.     

姜黄素对胶质瘤C6细胞垂体瘤转化基因下调作用的体外研究

目的 探讨姜黄素对体外培养胶质瘤C6细胞垂体瘤转化基因(PTTG)表达的影响.方法 体外培养胶质瘤C6细胞,给予不同浓度的姜黄素(10μmol/L、20 μmol/L、30μmol/L)作用24h后应用半定量逆转录聚合酶链式反应(RT-PCR)检测PTTG mKNA表达情况,Western blot方法 检测PTTG蛋白水平的变化. 结果 姜黄素作用后,C6细胞PTTG mRNA表达与PTTG蛋白水平均下降,差异有统计学意义(P<0.01),且随着浓度的增加,其下降越明显. 结论 姜黄素具有下调胶质瘤C6细胞PTTG表达的作用,并呈剂量依赖性.     

树突状细胞瘤苗抗脑胶质细胞瘤作用体外实验研究

目的通过体外实验探讨应用树突状细胞制备的肿瘤疫苗抗脑胶质瘤的作用机制.方法培养大鼠树突状细胞,冻融法制备胶质瘤抗原,以肿瘤抗原致敏树突状细胞而制备胶质瘤疫苗.将实验动物分为4组,分别将胶质瘤疫苗、树突状细胞、生理盐水注入正常大鼠体内,到达预定时间取出大鼠脾脏,四噻唑蓝(MTT)法检测大鼠淋巴细胞活性.结果一次输入胶质瘤疫苗的正常大鼠的淋巴细胞对肿瘤细胞的杀伤活性明显低于3次输入胶质瘤疫苗的正常大鼠;比较输入培养8 d的树突状细胞和输入生理盐水的大鼠,其淋巴细胞对肿瘤细胞的杀伤活性无统计学差异.结论应用树突状细胞制备胶质瘤疫苗可明显激活体内抗肿瘤免疫反应.     

Spontaneous regression of experimental gliomas--an immunohistochemical and MRI study of the C6 glioma spheroid implantation model

OBJECTIVE: The orthotopic C6 glioma spheroid implantation model has been used to examine factors of neoangiogenesis, growth factor release, and protease expression as well the effect of antitumor agents. The present study systematically investigates the long-term course of orthotopically implanted C6 spheroid gliomas. METHODS: Reaggregated C6 spheroid tumors were implanted into the forebrain of 48 male Sprague-Dawley rats (32 immunocompetent, 16 thymectomized). The animals were examined by MRI at postoperative day (POD) 7, 14, 21, 28, 32, 45, 60, and 70. The MRI protocol included a T2-w and T1-w SE sequence before and after application of contrast medium and a CISS 3D sequence for volumetry. A total of six animals were selected after each MR exam from both groups and sacrificed for HE light microscopy and CD8+ T-lymphocyte, ED1+ macrophage, CD31+ endothelial cell immunohistochemistry. RESULTS: The tumors progressed to reach a maximum volume on day 28: 0.23 +/- 0.05 ml in the thymectomized and 0.16 +/- 0.021 ml in the immunocompetent group. Tumors then consistently regressed to vanish completely by POD 70. The influx of cytotoxic CD8+ T-lymphocytes correlated with tumor progression and the tumors reached a larger size in the thymectomized group. However, the time course of tumor regression was the same for both groups. CONCLUSION: The present data suggest that the orthotopic C6 glioma implanted into Sprague-Dawley rats will progress within a time span of approximately 4 weeks and can then retrogress again spontaneously. This finding has to be taken into account when deciding on a study protocol and the appropriate animal model. The C6 glioma model may be suitable to study the cell biological steps involved in the phenomenon of spontaneous tumor regression.     

屏状核与其周围白质纤维束关系的显微解剖学研究

目的 对屏状核及其周围的白质纤维束进行显微解剖学关系研究,并分析其功能意义.方法 在4～25倍手术显微镜下,运用Klingler纤维剥离技术,解剖剥离10例被10％甲醛固定的成人尸头,观察屏状核及其周围白质的解剖结构.结果 屏状核分为两部分:位于后上的背侧屏状核及位于前下的腹侧屏状核.屏状核是一位于岛叶深部、为众多白质所包绕的不规则薄层状灰质,屏状核内侧和背侧是外囊纤维,外侧为最外囊纤维,腹侧是钩状束及枕额下束,后下为内囊的豆核下部纤维由内向外穿出.其中来自钩状束、枕额下束的纤维进入或横贯腹侧部屏状核,来自外囊的纤维起始或终止于背侧屏状核,来自最外囊的部分纤维起始或终止于背侧屏状核,来自内囊豆核下部的部分纤维进入或横贯屏状核后下极.结论 通过研究屏状核周围白质纤维束的解剖功能学或许可以为屏状核的功能研究提供一条新的思路.     

pEGFP-ING4抑制裸鼠皮下人U87移植瘤生长及肿瘤血管生成

目的 探讨pEGFP-ING4对裸鼠体内人U87细胞移植瘤生长及对肿瘤血管生成的影响.方法 将成功构建的18只人U87细胞裸鼠皮下移植瘤模型,随机分为pEGFP-ING4和pEGFP-C2转染组及PBS空白对照组3组,测量各组肿瘤的体积变化,荧光显微镜观察转染瘤体内绿色荧光蛋白(green fluorescent protein,GFP)的表达情况,免疫组织化学SABC法检测微血管密度(microvessel density,MVD).结果 荧光显微镜下pEGFP-ING4 组和pEGFP-C2组均观察到GFP表达,接种后第21d、28d、35d各组皮下肿瘤体积(mm3)为:PBS 组(201.8±19.3,418.9±26.4,622.1±51.3),pEGFP-C2 组(197.6±18.9,398.4±20.4,593.7±48.7),pEGFP-ING4 组(138.9±8.4,198.7±21.5,293.2±31.6),肿瘤接种后在同一时间点内,pEGFP-ING4 组肿瘤体积明显小于另外两组(P<0.05);MVD检测(个/mm2):PBS 组(15.83±0.98),pEGFP-C2 组(15.83±1.62),pEGFP-ING4 组(4.17±1.17),与另外两组比较,pEGFP-ING4 组MVD明显降低(P<0.01).结论 ING4基因能够明显抑制人U87细胞裸鼠皮下移植瘤的生长,抑制胶质瘤血管的生成可能是其抗肿瘤的重要机理之一.     

树突状细胞活化的CTLs对神经胶质瘤的体外杀伤作用研究

目的研究负载肿瘤抗原的树突状细胞(DCs)活化的特异性细胞毒性T淋巴细胞(CTLs)对神经胶质瘤细胞的体外杀伤效应,探讨其用于临床治疗的可行性。方法体外原代培养胶质瘤细胞,冻融法获取胶质瘤细胞抗原,联合应用粒细胞/巨噬细胞集落刺激因子、白细胞介素-4和肿瘤坏死因子-α等,对人外周血单核细胞进行体外诱导来获取DCs并负载肿瘤抗原,激活自体T淋巴细胞,制备特异性CTLs,MTT法检测对胶质瘤细胞的体外杀伤效应。结果负载胶质瘤抗原的DCs激活的CTLs对胶质瘤的杀伤作用显著高于对K562细胞的杀伤作用(P<0.01),并且杀伤活性随着效靶比的增加而增加;负载胶质瘤抗原的DCs激活的CTLs对胶质瘤的杀伤活性明显高于未经胶质瘤抗原致敏的DCs刺激的CTLs对胶质瘤的杀伤作用(P<0.01)。结论联合应用细胞因子从人外周血中诱导出的DCs负载胶质瘤抗原后,激活的CTLs在体外对胶质瘤细胞能产生高效而特异的杀伤作用,为临床应用DCs瘤苗奠定基础。     

干细胞样抗原提高树突状细胞介导胶质瘤免疫治疗的体外研究

目的 比较胶质瘤干细胞样抗原与非干细胞样抗原致敏树突状细胞(DCs)疫苗对胶质瘤的体外作用.方法 体外用无血清法和血清法培养恶性胶质瘤细胞,有限稀释法检测其克隆形成率;冻融法获取相应胶质瘤抗原;GM-CSF、IL-4体外诱导人外周血单核细胞获取DCs,与抗原孵育后再激活T淋巴细胞,同位素法检测效应细胞对胶质瘤细胞的杀伤率;流式细胞术检测DCs表面分子变化以及非贴壁细胞中T细胞比例的变化.结果 无血清培液中的胶质瘤细胞具有更高的克隆形成率(P<0.01);DCs经不同抗原刺激后HLA-A、HLA-DR、CD80、CD86表达上调(P<0.01);非贴壁细胞与DCs共培养后T细胞比例明显增高;干细胞样抗原激活的效应细胞对非干细胞样细胞和干细胞样细胞杀伤率分别为(70.2±5.13)%和(56.7±7.81)%(P<0.001),而非干细胞样抗原活化的效应细胞相应的杀伤率为(36.6±6.45)%和(9.05±3.49)%(P<0.001).结论 无血清培养液中的胶质瘤细胞具有更强的增殖能力,胶质瘤干细胞样抗原较传统抗原具有更强的抗原性,为进一步提高胶质瘤DCs疫苗疗效奠定基础.     

外源性TRAIL基因转染联合氯喹诱导胶质瘤U251细胞凋亡

目的探讨外源性肿瘤坏死因子凋亡相关诱导配体(TRAIL)联合氯喹对U251细胞凋亡的作用。方法构建稳定表达TRAIL的质粒p EGFP-TRAIL,然后转染到胶质瘤细胞系U251细胞中(TRAIL组),以p EGFP-C1质粒为阴性对照,以不转染质粒为空白对照;将氯喹(50μmol/L)加入到转染p EGFP-TRAIL质粒U251细胞培养基中,作为联合组。共聚焦显微镜检测GFP-TRAIL蛋白表达,MTT法检测细胞抑制率,Annexin V-FITC/PI双染法检测细胞凋亡,免疫印迹法检测GFP-TRAIL和Cleaved caspases-8蛋白表达。结果质粒转染后,共聚焦荧光显微镜检测结果显示,p EGFP-C1在细胞质中成弥散分布;而GFP-TRAIL在细胞质中成聚点分布,且荧光表达可以持续48 h以上;免疫印迹法分析结果显示TRAIL组TRAIL蛋白表达水平明显高于空白对照组和阴性对照组(P0.05)。联合组细胞增殖抑制率[(47.22±0.15)%]明显高于阴性对照组[(3.21±0.04)%,P0.05]和TRAIL组[(23.88±0.22)%,P0.05]。联合组细胞凋亡率[(41.62±0.44)%]明显高于空白对照组[(2.14±0.09)%,P0.05]、阴性对照组[(3.46±0.17)%,P0.05]和TRAIL组[(22.48±0.43)%,P0.05]。联合组Cleaved caspases-8蛋白表达水平明显高于阴性对照组、TRAIL组(P0.05)。结论外源性TRAIL基因转染后可以在细胞中稳定表达并诱导细胞凋亡,与氯喹联合应用可以增强TRAIL诱导的细胞凋亡,其机制可能是氯喹抑制细胞自噬。     

Effects of an intratumoral injection of human recombinant tumor necrosis factor-α on cerebrovascular permeability and leukocytic infiltration in a rat glioma model

The pro-inflammatory and blood-brain barrier (BBB) effects of intratumoral (IT) injection of human recombinant tumor necrosis factor-α (rTNF-α) were studied in the Fischer rat RT-2 glioma model. Animals received a single stereotaxic injection of either 6×10⁴ U rTNF-α or excipient (vehicle) into the center of an intracerebrally implanted glioma. In order to demonstrate any effects rTNF-α might have on the BBB, studies were conducted using endogenous IgG (150 kD) as a tracer. Forty-eight hours following injection of excipient, a margin of peritumoral IgG extravasation was observed while rats treated with 6×10⁴ U rTNF-α showed a dense and extensive IgG extravasation involving both hemispheres. Histological examination revealed that an IT rTNF-α injection induced leukocytic adherence, neutrophilic cuffing and infiltration throughout the lesion from 12 to 72 h after injection. These histological observations were supported by quantification of cerebral myeloperoxidase (MPO) levels which indicated a significant increase in neutrophils over the excipient recipients at 4 and 12 h. These MPO levels contrasted with our earlier studies in normal rats which revealed no significant difference in tissue MPO levels following injection of excipient or rTNF-α. In addition, when MPO levels in tumor models and normal rats receiving TNF were compared, a significantly greater presence of neutrophils was seen in tumor models at 12 h post-TNF injection. We believe that the increased inflammatory response seen in a progressing glioma compared to normal brain may be the result of decreased resistance to leukocytic infiltration due to increased vascular surface area, the lack of infiltration-resistant perivascular basement membrane, and/or increased extracellular space. Received: 8 June 1995 / Revised: 19 March 1996 / Accepted: 17 June 1996     

白藜芦醇抑制SHG-44胶质瘤细胞生长实验研究

目的探讨白藜芦醇(Res)在体外诱导脑胶质瘤SHG-44细胞凋亡并抑制其生长的作用。方法四甲基偶氮唑蓝(MTT)比色法测量不同剂量的Res作用6h、24h和48h后对SHG-44胶质瘤细胞增殖的影响。HE染色、Hoechst33342荧光染色观察细胞形态改变,DNAladder检测细胞DNA裂解情况,流式细胞仪用异硫氰酸荧光素标记的膜联蛋白V(AnnexinV-FITC)和碘化丙啶(PI)双染检测凋亡率,并测定细胞周期的改变。结果Res明显抑制SHG-44细胞的生长和增殖(P<0.01),呈浓度及时间依赖性反应;Res所致的SHG-44胶质瘤细胞凋亡为浓度依赖关系,随着浓度的增高,凋亡更明显。此凋亡细胞周期主要发生G1期比例升高,S、G2期比例降低。结论Res明显抑制SHG-44细胞生长并诱导其发生凋亡和细胞周期改变,为Res用于治疗脑胶质细胞瘤提供了实验依据。     

诺帝诱导人胶质瘤细胞系CHG-5分化的蛋白质表达图谱的建立及差异蛋白质组分析

目的建立诺帝诱导人胶质瘤细胞系CHG-5分化的蛋白质表达图谱并比较其表达差异。方法分别用100μmol/L、200μmol/L诺帝诱导CHG-5细胞分化,观察处理后24h、48h、72h细胞的形态改变;分别提取200μmol/L诺帝处理后的CHG-5细胞和对照组细胞总蛋白,进行双向电泳,所获蛋白表达图谱用PDquest 7.1软件比较其蛋白质表达差异,选取部分高表达的差异蛋白进行质谱分析。结果100pμmol/L和200μmol/L诺帝处理后均可明显诱导CHG-5细胞分化,以72h时细胞分化最为明显。双向电泳发现诱导分化后细胞出现了18个差异蛋白点,其中9个蛋白点表达上调,9个蛋白点表达下调,并获得差异蛋白的分子量、等电点等信息。对其中部分高表达的差异蛋白点成功地进行了质谱鉴定。结论诺帝诱导人胶质瘤细胞系CHG-5分化时蛋白质组改变涉及到细胞增殖、凋亡、基因转录、蛋白表达调控等各个方面。     

Observations on the lesions in the white matter of the spinal cord of swayback sheep

Summary Eleven sheep with swayback aged 2, 3 1/2, 6 1/2 and 8 months, and 10 normal sheep of similar age were examined. The histopathology and biochemistry of the spinal cord white matter was studied in detail. There was less myelin in the spinal cord white matter of the swayback animals and it had a lower content of cerebroside and ethanolamine phospholipid than that of the controls. Myelin breakdown products were only found in the older animals and there was a close correlation between the results obtained by the OTAN, Sudan II and Marchi techniques. These results are discussed in relation to the pathogenesis of the lesion.

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Zusammenfassung Elf Schafe mit Swayback im Alter von 2, 3 1/2, 6 1/2 und 8 Monaten sowie 10 Normaltiere gleichen Alters wurden untersucht. Die Histopathologie und Biochemie der weißen Substanz des Rückenmarks wurde ausführlich studiert. Im Rückenmark der kranken Tiere lag weniger Myelin und ein geringerer Gehalt an Cerebrosiden und Ethanolamin-Phospholipid als in den Kontrollen vor. Markscheidenzerfallsprodukte wurden nur bei älteren Tieren gefunden, wobei gute Übereinstimmung der Ergebnisse der OTAN-, Sudan II- und Marchi-Methoden bestand. Die Befunde werden hinsichtlich ihrer Beziehung zur Pathogenese der Läsion diskutiert.

     

ZFNet模型在胶质瘤MRI诊断中的应用

研究背景胶质瘤疾病负担较重,尽早确诊和及时治疗可有效延长无进展生存期,临床实践中初诊疑似胶质瘤时首选头部MRI检查,人工阅片存在诊断结果不一致和阅片效率下降的缺陷,而通过深度学习算法进行医学影像识别与诊断成为可能.本研究采用人工神经网络相关机器学习算法,辅助影像科医师对胶质瘤患者头部MRI图像的人工阅片,以期改善人工阅...     

人脑胶质瘤干／祖细胞系SU-2放射耐受及其相关机制的初步研究

目的研究胶质瘤干细胞在胶质瘤耐受放射中的作用,为克服恶性胶质瘤放射耐受寻找新的干预靶点。方法干细胞培养条件下培养自建人脑胶质瘤干/祖细胞系SU-2,以及人脑胶质瘤细胞系U251和SHG-44,观察不同剂量直线加速器照射前后细胞形态变化、Hoechst 33342-细胞和CD133 细胞比例、肿瘤细胞存活率和裸小鼠致瘤率等项指标,并以实时荧光定量PCR方法检测人脑胶质瘤细胞系U251和人脑胶质瘤干/祖细胞系SU-2照射前后MGMT基因表达水平。结果当照射剂量为1～15Gy时,人脑胶质瘤干/祖细胞系SU-2中的Hoechst 33342-细胞和CD133 细胞比例明显增加,高达18.73%和13.70%,细胞存活率升高、致瘤率(8/8)增加、侵袭性增强,MGMT基因表达水平轻度升高。结论经一定剂量的X线照射后,胶质瘤干细胞因具有强于其他肿瘤细胞的放射耐受性而出现选择性存活且细胞比例升高,其生物学特性如细胞存活率、体内致瘤率增加,侵袭性增强。可能与胶质瘤干细胞DNA损伤修复能力提高有关,确切的分子学机制值得进一步深入研究。     

内抑制素对人脑胶质瘤细胞增殖的实验研究

目的观察内抑制素对人脑胶质瘤细胞增殖的影响。方法①细胞培养：体外培养人脑胶质瘤细胞并进行光镜观察;②抑制因子试验：设立对照组与内抑制素实验组,采用四甲基偶氮唑蓝（MTT）法来判定该抑制因子对人脑胶质瘤细胞有无抑制作用;③细胞内游离Ca2＋浓度测定：将特异性ca2＋荧光指示剂-Fluo-3用来负载人脑胶质瘤细胞,应用激光共聚焦显微镜检测细胞内游离钙的浓度。结果对照组光密度（OD）值明显高于各实验组（P〈0．05）;内抑制素能明显增加细胞内游离Ca2＋的浓度,并且随药物浓度的增加而增加。结论内抑制素能够抑制人脑胶质瘤细胞的增殖且呈剂量依赖性,同时也能够增加胶质瘤细胞内游离ca2＋的浓度。