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目的:分析不同亚型高危型人乳头瘤病毒(high riskhumanpapillomavirus,HR HPV)的感染情况与不同级 别宫颈病变中的相关性,探讨 HR HPV在宫颈癌前病变及宫颈癌发生发展中的作用。方法:回顾性收集 2016年 1月 至 2020年 12月在海南省海口市妇幼保健院行阴道镜检查及镜下活检的 591例宫颈病变患者作为研究对象,根据病 理检查结果按照宫颈病变严重程度将患者分为慢性宫颈炎(chroniccervicitis,CC)组(195例)、低级别鳞状上皮内病 变(low gradesquamousintraepitheliallesion,LSIL)组(236例)、高级别鳞状上皮内病变(high gradesquamousintraepi theliallesion,HSIL)组(150例)、鳞状细胞癌(squamousofcervicalcarcinoma,SCC)组(10例),回顾性分析各组 HR HPV感染情况,对不同 HR HPV感染亚型与宫颈病变程度进行相关性分析。结果:CC、LSIL、HSIL和 SCC组患者的 HR HPV感染率分别为 28.2%(55/195)、79.7%(188/236)、93.3%(140/150)、100.0%(10/10),随着宫颈病变严重 程度的增加,HR HPV感染率也随之增加,差异有统计学意义(χ2趋势 =172.464,P<0.001)。不同级别宫颈病变组, 随着宫颈病变严重程度的增加,HR HPV单一感染率也随之增加,差异有统计学意义(P<0.05),HR HPV多重感染 率之间随病变严重程度的增加亦呈升高趋势,但在 SCC组的感染率稍有回落(P<0.05)。在不同级别宫颈病变患者 及全部宫颈病变患者中,感染率最高的亚型均为 HPV16,且 HPV16的感染率随着病变严重程度的增加而升高,差异 具有统计学意义(χ2趋势 =14.805,P<0.001)。结论:HR HPV感染率与宫颈病变的严重程度呈正相关关系,不同宫 颈病变严重程度组患者 HR HPV的单一感染均明显大于多重感染率,本地区导致宫颈癌及癌前病变感染率最高的 5 种亚型依次为 HPV16、52、58、18、51。 相似文献
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目的探讨宫颈病变与HPVDNA基因型感染的关系。方法导流杂交基因分型技术检测HPVDNA。结果炎症、CINI、CINII、CINIII及宫颈癌中HPV感染率分别为62.24%、67.64%、91.67%、96.87%、96.00%;宫颈病变中6种最常见的HPV基因型为16、18、52、58、33、31。炎症、CINI、CINII、CINIII及宫颈癌患者多重感染率分别为19。0%、35.8%、45.9%、54.9%、60.8%、70.2%。结论随宫颈病变程度加重HPV感染率增加,宫颈病变中6种最常见HPV基因型为16、18、52、58、33、31。多重HPV感染可能促进宫颈癌的发生。 相似文献
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宫颈高级别病变与HPV感染型别分析 总被引:1,自引:0,他引:1
目的探讨HPV在宫颈高级别病变中的感染率及感染型别。方法采用导流杂交法分别检测CINII~Ⅲ30例和宫颈癌患者160例HPV基因型别,比较HPV感染与宫颈病变的关系。结果CINⅡ~III和宫颈癌患者HPV感染率均为90%,且以单型别感染为主,分别为70.37%(19/27)、81.94%(118/144);在CIN II~Ⅲ中HPV58型、52型感染居多,宫颈癌则以HPV16型、18型感染最常见;无论宫颈鳞癌还是宫颈腺癌,以HPV16型检出率最高。结论HPV16型、18型是宫颈癌的主要致病型,不同病理类型并无HPV型别上的差异;宫颈上皮高级别内瘤变则以HPV58型、52型感染为主;对HPV58型、52型感染者应重视随访。 相似文献
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深圳女性人群生殖道HPV感染与宫颈病变相关性 总被引:1,自引:0,他引:1
[目的]了解深圳女性人群生殖道HPV感染与液基细胞学异常及宫颈上皮内瘤样病变间相关性。[方法]采取整群分层抽样的方法,在深圳市南山区沙河街道办事处辖区随机选择15~59岁智力正常的妇女为研究对象。研究对象1137例接受问卷调查、妇科检查、HPV检测、液基细胞学和阴道镜检查。[结果]研究人群HPV阳性率为14.0%,宫颈液基细胞学异常率13.6%,CIN现患率为4.4%。不同细胞学类型和不同病理类型的HPV阳性率有显著性差异(P〈0.01),细胞学和病理学病变程度均与HPV滴度呈正相关(P〈0.01)。[结论]随着HPV阳性率与病毒载量水平的增高,宫颈细胞学和组织学病变程度均加重。深圳妇女的宫颈病变尚处于发病的早期阶段,预防HPV感染至关重要。 相似文献
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[目的]了解高危型人乳头瘤病毒感染与宫颈病变的关系。[方法]对杭州市萧山区20~60岁常住妇女4446人进行妇科检查及宫颈HPV检测,并行宫颈细胞学检查,分析高危型人乳头瘤病毒感染与宫颈病变间相关性。[结果]HPV感染者462例,HPV总感染率为10.39%(462/4446),其中HR-HPV感染431例,HR-HPV感染率为9.69%(431/4446)。HR-HPV感染阳性者宫颈细胞学异常的检出率为40.13%(173/431);而HR-HPV阴性组中,宫颈细胞学异常的检出率为6.37%(256/4015),两者比较差异有统计学意义(χ2=508.443,P〈0.001,OR=9.838,95%CI:7.813~12.388)。[结论]HR-HPV感染是宫颈病变的重要因素。 相似文献
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子宫颈病变HPV感染与端粒酶活性相关性研究 总被引:1,自引:0,他引:1
目的探讨人乳头状瘤病毒(human papillomavirus type,HPV)感染与端粒酶活化在各种子宫颈病变中的关系.方法对146例子宫颈病变应用TRAP-PCR银染定性及TRAP-PCR-ELISIA定量法检测端粒酶活性,PCR法检测HPV分型;其中子宫颈癌43例,子宫颈上皮内瘤变(CIN)47例(CIN Ⅰ~Ⅱ级39例,其中子宫颈湿疣28例;CIN Ⅲ级8例),子宫颈炎56例(轻度子宫颈炎11例,中重度子宫颈炎45例),另取正常子宫颈标本10例做对照.结果 146例子宫颈病变标本中检测结果为:(1)端粒酶阳性率和HPV16/18型阳性率:子宫颈癌>子宫颈CIN Ⅲ级>CIN Ⅰ~Ⅱ级>子宫颈炎>正常子宫颈(P<0.05);HPV6/11型阳性率:CIN Ⅰ~Ⅱ级>CIN Ⅲ级(P<0.001).(2)子宫颈癌中端粒酶阳性率:Ⅰ期<Ⅱ期(P<0.05),高分化组<中分化组(P<0.01);HPV16/18阳性率:Ⅰ期<Ⅱ期(P<0.025),高分化组<中分化组(P<0.05);子宫颈腺癌与鳞癌端粒酶及HPV差异无显著性(P>0.05).(3)端粒酶阳性值及HPV6/11、HPV16/18拷贝数:子宫颈癌>子宫颈CIN(CIN Ⅲ级>CIN Ⅰ~Ⅱ级)>子宫颈炎(P<0.001).(4)4例端粒酶及HPV均阳性子宫颈CIN Ⅰ~Ⅱ级病例经治疗后,端粒酶阳性率差异无显著性(P>0.05),端粒酶活性及HPV拷贝数下降差异有显著性(P<0.05,HPV6/11:P<0.01).结论 HPV感染尤以16/18型和端粒酶的激活与子宫颈病变恶变关系密切,其可能通过激活端粒酶诱发癌变. 相似文献
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目的 通过分析山东青岛地区宫颈组织中HPV16型E6和E2基因突变情况,探讨其与该地区宫颈癌的关系。方法 从104例青岛地区宫颈疾病组织中提取DNA作为模板,采用聚合酶链式反应(PCR)技术筛选出高危型HPV及HPV16阳性标本,扩增出HPV16型E6、E2全长基因,PCR产物纯化后测序,与德国HPV标准株进行比对分析。结果 宫颈组织中高危型HPV阳性率为93.27%(97/104),HPV16阳性率为69.23%(72/104)。HPV16阳性标本中扩增出E6基因37例,有5例与标准株序列相同,32例存在突变,其中25例突变型别为T178G或T178A(D25E)。在E2基因全序列测序中,23例均存在C3684A(T-K),14例同时存在T3524C、C3684A(T-K)和C3787A(D-E),9例同时存在A2926G、C3159A(T-K)、G3249A(R-Q)、T3384C(I-T)、C3410T(P-S)和C3684A(T-K)。结论 山东青岛地区宫颈癌患者HPV16型E6、E2基因与德国标准株比较存在多处变异,E6与E2基因突变可能存在相关性。 相似文献
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目的 探讨女性宫颈高危型人乳头瘤病毒(hrHPV)感染现状并分析hrHPV病毒载量与宫颈病变之间剂量—效应关系。方法 对26294份宫颈细胞学送检标本采用杂交捕获第二代方法(HC-Ⅱ)检测HPV-DNA含量,病毒载量以相对光单位(RLU)与界值点(Cutoff)比值衡量。以RLU/CO≥1.0为阳性。宫颈病变按组织学活检以CIN2及以上级别病变为病例。假设检验采用χ2检验;用Mantel趋势分析分析剂量—效应关系。结果 26294例受检对象总体阳性检出率为26.74%,年龄分布在15~84岁之间,平均年龄为(33.54±8.43)岁。hrHPV病毒载量与宫颈病变存在剂量—效应关系(χtrend=8.000,P<0.001)。结论 广东省女性hrHPV感染阳性检出率较高;hrHPV病毒载量与宫颈病变之间存在剂量—效应关系。 相似文献
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目的探讨HPV感染与宫颈病变发生的关系。方法将病理检查确诊宫颈有病变的187例患者分为3组:慢性炎症组(n=41)、宫颈上皮瘤变(CIN)组(n=62)和宫颈癌组(n=84)。采用人乳头瘤病毒核酸扩增分型技术对患者宫颈脱落细胞样本作HPV基因分型检测,分析HPV感染状况及HPV基因型在各组疾病中的分布。结果 1187例患者中共检出HPV阳性者136例,阳性率为72.72%(136/187),其中高危型HPV感染阳性率为95.58%(130/136)。在被测的21个HPV亚型中最常见的6个类型依次为16型(46.32%、63/136),58型(21.32%、29/136),52型(15.44%、21/136),18型(12.50%、17/136),33型(9.56%、13/136),31型(8.09%、11/136),未检测出44型、43型及6型。2慢性炎症组HPV阳性率为14.63%(6/41);CIN组HPV阳性率为85.48%(53/62)、其中CINⅠ组、CINⅡ组、CINⅢ组中HPV感染率分别为62.50%(10/16)、90.47%(19/21)、96.00%(24/25);宫颈癌组HPV阳性率91.67%(77/84)。CIN及宫颈癌组HPV阳性率均高于炎症组,而CINⅡ~CINⅢ组及宫颈癌组HPV阳性率又明显高于CINⅠ组。3组阳性表达率差异有统计学意义(P<0.05)。3HPV阳性者中单一感染率为67.65(92/136),多重感染率为32.35%(44/136),其中以双重感染为主,占79.55%(35/44)。4HPV感染患者年龄以30~59岁最高,但各年龄段HPV感染检出率比较无统计学差异(P>0.05)。5慢性炎症组、CIN组和宫颈癌组的16型感染率分别为16.67%(1/6)、47.16%(25/53)、48.05%(37/77)。CIN组和宫颈癌组的16型感染率高于慢性炎症组,差异具有统计学意义(P<0.05)。结论 HPV感染与宫颈病变发生、发展密切相关,HPV基因分型诊断对宫颈病变高危人群的筛查、预防、临床诊断、治疗可提供重要的理论依据。 相似文献
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Cervical cancer is one of the most common types of cancer in women worldwide, with the highest rates observed in underdeveloped
countries. In the last decades, its incidence has decreased after the implementation of screening programs, mainly in developed
countries. Iinfection with high-risk oncogenic HPV is associated with precancerous lesions and cervical cancer. Advances in
the understanding of the role of HPV in the etiology of high-grade cervical lesions (CIN 2/3) and cervical cancer have led
to the development, evaluation and recomendation of two prophylactic HPV vaccines. This review article provides a summary
of the studies related with their development and efficacy. 相似文献
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Objective: Cervical cancer (CC) is the second most common type of cancer in women worldwide, after breast cancer. High-risk human papillomaviruses (HR-HPVs) are considered to be the major causes of cervical cancer. HPV16 is the most common type of HR-HPVs and HPV16 E6 gene is one of the major oncogenes. Specific mutations are considered as dangerous factors causing CC. This study was designed to find mutations of HPV16 E6 and the relationship between the mutations and the happening of CC.Methods: The tissue DNA was extracted from 15 biopsies of CC. Part of HPV16 E6 gene (nucleotide 201-523) was amplified by polymerase chain reaction (PCR) from the CC tissue DNA. The PCR fragments were sequenced and analyzed.Results: The result of PCR showed that the positive rate of HPV16 E6 was 93.33% (14/15). After sequencing and analyzing, in the 13 out of 14 PCR fragments, 4 maintained prototype (30.77%), 8 had a same 350G mutation (61.54%), and 1 had a 249G mutation (7.69%).Conclusion: This study suggest that there is a high infection rate of HPV in cervical cancer and most of the HPV16 E6 gene has mutations. Those mutations may have an association with the development of cervical cancer. 相似文献
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Cervical cancer (CxCa) is the second most common cancer among women globally. Human papillomavirus (HPV) infection is thought to be a necessary, but not sufficient, causal factor in CxCa development. Why some women are able to clear HPV infection with no adverse effects, whereas others develop cancer, remains unclear. HHV-6 has demonstrated transformative abilities and has been shown to be present in the genital tract. However, based on the current evidence, we cannot conclude that HHV-6 is a co-factor in HPV-associated carcinogenesis. Nonetheless, future research is warranted because of several crucial gaps in the literature. 相似文献
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新疆维吾尔族妇女宫颈癌组织中HPV16型E6基因突变分析 总被引:9,自引:2,他引:9
背景与目的:高危型人乳头状瘤病毒16和18(human papillomavirus type16 and 18,HPV16,HPV18)是宫颈癌主要病因之一,尤其以HPV16最为常见,其中HPV16E6是主要癌基因之一。在一些地区,特定的E6基因突变株是宫颈癌发生的危险因素。新疆南部维吾尔族聚居区足宫颈癌高发区,我们已在前期的研究中发现该地区HPV16E6基因发生突变。本研究旨在检测该突变在新疆南部维吾尔族妇女宫颈癌组织中的分布规律,并探讨其与该地区宫颈癌高发的关系。方法:从35例中国新疆南部维吾尔族妇女宫颈癌活检标本中提取组织DNA作为模板,PCR扩增HPV16E6全长基因,PCR产物直接测序或克隆后测序,分析新疆维吾尔族妇女宫颈癌组织中HPV16E6基因的突变。结果:PCR检测结果表明宫颈癌组织中HPV16E6阳性率为82.86%(29/35);26例中E6分离片段的测序和序列分析表明,15例(57.69%)分离株E6基因与原型相同,另有11例(42.31%)E6基因突变,其中9例(34.62%)分离株发生了L83V突变,2例(7.69%)分离株发生rL83V/D63E突变。结论:中国新疆南部地区HPV16E6基囚发生变异,其原型和变异型在该地区维吾尔族宫颈癌患者巾的分布规律可能与该地区宫颈癌高发存在一定关系。 相似文献
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目的探讨女性人乳头瘤病毒(HPV)感染患者的基因型分布特征。方法对1623例患者采用导流杂交法进行21种HPV基因亚型检测。结果①1623例患者中有798例检测出HPV感染,HPVDNA阳性率为49.2%。30—39岁患者HPVDNA阳性率(54.3%)最高,20—29岁患者HPVDNA阳性率(27.3%)最低。@798例HPV感染患者中620例(77.7%)患者单一基因型HPV感染,178例(22.3%)患者多重基因型HPV感染。③除HPV43型以外,其余20种亚型均被检测出,其中以HPVl6型(55.4%)阳性率最高,其余依次为HPV52型(14.9%)、HPV58型(13.7%)、HPVl8型(9.1%)、HPV33型(7.5%)等。结论年龄与HPV阳性率有一定的关系。本地区HPV感染患者以单一基因型感染最为常见,主要高危型包括HPVl6型、HPV52型、HPV58型等。 相似文献
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De Vuyst H Gichangi P Estambale B Njuguna E Franceschi S Temmerman M 《International journal of cancer. Journal international du cancer》2008,122(1):244-246
To evaluate the fraction of invasive cervical carcinoma (ICC) that could be prevented in HIV-infected women by vaccines currently available against human papillomavirus (HPV)16 and 18, we conducted a cross-sectional study in women with ICC in Nairobi, Kenya. Fifty-one HIV-positive women were frequency-matched by age to 153 HIV-negative women. Cervical cells were tested for HPV DNA using polymerase chain reaction-based assays (SPF10-INNO-LiPA). Comparisons were adjusted for multiplicity of HPV types. As expected, multiple-type infections were much more frequent in HIV-positive (37.2%) than in HIV-negative (13.7%) women, but the distribution of HPV types was similar. HPV16 was detected in 41.2% versus 43.8% and HPV16 and/or 18 in 64.7% versus 60.1% of HIV-positive versus HIV-negative women, respectively. The only differences of borderline statistical significance were an excess of HPV52 (19.6% versus 5.2%) and a lack of HPV45 (7.8% versus 17.0%) in HIV-positive women compared to HIV-negative women, respectively. We have been able to assess an unprecedented number of ICCs in HIV-positive women, but as we did not know the age of HIV acquisition, we cannot exclude that it had occurred too late in life to affect the type of HPV involved in cervical carcinogenesis. However, if our findings were confirmed, they would suggest that the efficacy of current vaccines against HPV16 and 18 to prevent ICC is similar in HIV-positive and HIV-negative women, provided vaccination is administered before sexual debut, as recommended. 相似文献
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新疆维吾尔族妇女宫颈癌组织HPV16型E6基因变异分析 总被引:1,自引:0,他引:1
目的:新疆维吾尔族妇女宫颈癌的发生主要与人乳头状瘤病毒16(HPV16)感染相关,特定的HPV16 E6突变株具有更高的致癌危险性.本研究通过检测维吾尔族妇女宫颈癌组织中HPV16 E6基因突变情况,探讨其与维吾尔族妇女宫颈癌高发的关系.方法:从140例维吾尔族妇女宫颈癌石蜡包埋组织中提取DNA作为模板,PCR 扩增HPV16 E6 全长基因,PCR 产物直接测序,进行突变分析.结果:本组维吾尔族妇女宫颈癌组织中HPV16的阳性率是73.6%(103/140),91例E6基因测序及分析结果表明,维吾尔族妇女宫颈癌组织中存在HPV16 E6变异株,其中49例(53.8%)分离株发生L83V突变,4例(4.39%)发生D25E突变,2例(2.20%)发生D64E/L83V突变,36例(39.6%)与野生型相同.结论:新疆维吾尔族妇女宫颈癌患者HPV16 E6基因发生变异,主要以L83V突变为主,HPV16变异株在维吾尔族妇女宫颈癌患者中的分布可能与维吾尔族妇女宫颈癌高发存在一定关系. 相似文献
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Stark A Gregoire L Pilarski R Zarbo A Gaba A Lancaster WD 《Cancer Detection and Prevention》2008,32(1):15-22
Background: Human papillomavirus (HPV) is the major risk factor for cervical cancer. Methods: We implemented a retrospective case-series study to discern HPV knowledge accuracy among women diagnosed with and treated for cervical cancer. Cases (n = 1174), identified from the Pathology database, were diagnosed and treated for cervical cancer at the same institution. Data were collected using self-administered questionnaires and by reviewing medical records. Results: A total of 328 (27.9%) women returned the completed forms. Only 19% of the respondents had identified HPV as the primary risk factor for cervical cancer. Environmental pollutants, radiation exposure, poor dietary habits, excessive physical activity and family history of cervical cancer were listed as risk factors among many others. Multivariate analysis was performed to determine variables that were best associated with HPV knowledge accuracy. Age and education were the two variables that were statistically associated with the outcome. Younger and more educated women who participated in this study were more likely to know about the association between HPV infection and the risk of cervical cancer. Conclusions: Cervical cancer risk factor knowledge, especially knowledge about HPV is low, even among women with the history of cervical cancer. Younger and more educated women are more likely to have HPV and cervical cancer knowledge accuracy. The importance of personal health practices and the focus on health education should be equally emphasized to achieve successful cancer prevention through vaccination. 相似文献
20.
背景与目的:高危型人乳头状瘤病毒(high risk human papillomavirus,hrHPV)感染对于浸润性宫颈癌(invasive cervical carcinoma,ICC)及其癌前病变的致病性存在亚型及地区差异。本研究通过分析本地区30岁及以上女性宫颈病变患者中HPV亚型分布特点,进一步识别罹患包含ICC的高级别宫颈上皮内瘤变(cervical intraepithelial neoplasia grade 2 or worse,CIN2+)风险更高的亚型,并比较这些高风险亚型及亚型组合检测CIN2+病变的有效性。方法:收集来自复旦大学附属肿瘤医院就诊患者具备组织学随访结果的宫颈标本,行PCR-反向点杂交法(PCR-reverse dot blot,PCR-RDB)HPV基因分型检测,利用Logistic回归模型分析hrHPV亚型与CIN2+病变的风险关系,并构建ROC曲线(receiver operating characteristiccurve,ROC curve)评价不同亚型及亚型组合检测CIN2+病变的准确性。结果:符合研究要求患者413例,含38例CIN1,184例CIN2/3,126例ICC和65例阴性对照人群。HPV16、58、33和18依次为致CIN2+最常见的4种HPV亚型,仅HPV16(P<0.000 1)、58(P=0.002)及33(P=0.015)为罹患CIN2+病变的高风险亚型。联合检测HPV16/18/33/58诊断CIN2+病变的ROC曲线下面积(the area under the ROC curve,AUC)显著高于HPV16/18亚型组合(P=0.006 6)。结论:HPV16/18/33/58亚型组合用于辅助本地区CIN2+病变的检测可能更为有效。 相似文献