共查询到5条相似文献,搜索用时 15 毫秒
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Serdar Nasir Aleksandra Klimczak Erhan Sonmez Mehmet Bozkurt Sarah Gibson Maria Siemionow 《Transplant international》2010,23(1):90-100
Vascularized bone marrow transplant (VBMT) induces donor-specific chimerism in experimental models across the major histocompatibility barrier. An experimental model for immunotolerance studies should sustain a high antigenicity with low morbidity. Accordingly, we introduced an iliac bone osteomusculocutaneous (IBOMC) transplant model in rat. It consists of a large skin component and an abundant bone marrow cells (BMC) population. We tested this model with isograft transplantations between Lewis rats (RT1l ) and with allograft transplantation between Lewis-Brown Norway (LBN RT1l + n ) donors and Lewis (RT1l ) recipients under low dose of cyclosporine A monotherapy. Immunologic responses were tested for donor cell engraftment and chimerism induction. All isografts survived indefinitely and allografts were viable at 200 days post-transplant under low dose of cyclosporine A. Microangiography of the graft revealed preservation of skin, muscle, and bone components. Histologic examination confirmed viability of all allograft components without signs of rejection. Long-term engraftment of donor-origin (RT1n ) BMC was confirmed by donor-specific chimerism (1.2%) in peripheral blood and bone marrow (1.65%) compartments and by engraftment into lymphoid organs of recipients. The IBOMC transplant proved to be a reliable composite tissue allotransplantation (CTA) model. Moreover, because of its robust bone marrow component and large skin component, it is applicable to studies on immunologic responses in CTA. 相似文献
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Recipient bone marrow‐derived stromal cells prolong graft survival in a rat hind limb allotransplantation model 下载免费PDF全文
Ryosuke Ikeguchi Ryosuke Kakinoki Souichi Ohta Hiroki Oda Hirofumi Yurie Yukitoshi Kaizawa Hiroto Mitsui Tomoki Aoyama Junya Toguchida Shuichi Matsuda 《Microsurgery》2017,37(6):632-640
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Pedicled full‐thickness abdominal flap combined with skin grafting for the reconstruction of anterior chest wall defect following major electrical burn 下载免费PDF全文
Successful reconstruction of extensive anterior chest wall defect following major electrical burn represents a very challenging surgery. Herein we report the first case using pedicled full‐thickness abdominal flap combined with skin grafting to treat this injury with severe infection and exposure of pericardium and ribs in a Chinese patient. Following the performance of chest debridement to remove necrotic and infected tissues and the injection of broad‐spectrum antibiotics to reduce infection, a pedicled full‐thickness abdominal flap was used to cover the exposed pericardium and ribs, and skin grafting from the right leg of the patient was done to cover the exposed vital tissues. The patient was followed up for a total of 3·5 years, and satisfactory cosmetic and functional outcomes were obtained without complications. This report provides an effective method for the surgeons who encounter similar cases where reconstruction of extensive anterior chest wall is required. 相似文献
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Higher Anti‐A/B isoagglutinin titers of IgG class,but not of IgM,are associated with increased red blood cell transfusion requirements in bone marrow transplantation with major ABO‐mismatch 下载免费PDF全文
Gil Cunha De Santis Aline Cristina Garcia‐Silva Giuliana Martinelli Dotoli Pamela Tinti de Castro Belinda Pinto Simões Dimas Tadeu Covas 《Clinical transplantation》2017,31(4)