共查询到4条相似文献,搜索用时 15 毫秒
1.
Hausner Sven H. Bauer Nadine Davis Ryan A. Ganguly Tanushree Tang Sarah Y. C. Sutcliffe Julie L. 《Molecular imaging and biology》2020,22(6):1543-1552
Molecular Imaging and Biology - The αvβ6-BP peptide selectively targets the integrin αvβ6, a cell surface receptor recognized as a prognostic indicator for several challenging... 相似文献
2.
Hua Wu Haojun Chen Dongfeng Pan Yufei Ma Sheng Liang Ying Wan Ya Fang 《Molecular imaging and biology》2014,16(6):781-792
Purpose
Radiolabeled Arg-Gly-Asp (RGD) and Ala-Thr-Trp-Leu-Pro-Pro-Arg (ATWLPPR) peptide analogs have received interests for their capability to serve as radiopharmaceuticals for imaging integrin αvβ3 and Neuropilin-1 (NRP-1) positive tumors, respectively. In this study, we developed a RGD-ATWLPPR heterodimeric peptide which contained both RGD and ATWLPPR motifs in one molecular probe. The aim of this study was to investigate the dual receptor-targeting property and tumor diagnostic value of RGD-ATWLPPR heterodimeric peptide labeled with fluorine-18 (F-18).Procedures
A RGD-ATWLPPR heterodimer was synthesized from c(RGDyK) and ATWLPPR through a glutamate linker. The peptide was radiolabeled by reacting the [18F]fluoride–aluminum complex with the cyclic chelator, 1,4,7-triazacyclononane-1,4,7-triacetic acid (NOTA). The receptor-binding characteristics and tumor-targeting efficacy of [18F]FAl-NOTA-RGD-ATWLPPR were tested in vitro and in vivo.Results
RGD-ATWLPPR had affinity for both integrin αvβ3 and NRP-1 in vitro. [18F]FAl-NOTA-RGD-ATWLPPR displayed significantly higher tumor uptake than [18F]FAl-NOTA-RGD and [18F]FAl-NOTA-ATWLPPR, both in vitro and in vivo. The uptake of the F-18 labeled heterodimer by an U87MG tumor was inhibited only partially in the presence of an excess amount of unlabeled RGD or ATWLPPR but was blocked completely in the presence of both RGD and ATWLPPR. Compared with the monomeric RGD and ATWLPPR peptides, [18F]FAl-NOTA-RGD-ATWLPPR showed improved in vivo pharmacokinetics, resulting in a more preferable imaging quality.Conclusions
[18F]FAl-NOTA-RGD-ATWLPPR exhibited significantly improved receptor-targeting properties both in vitro and in vivo compared with the F-18 labeled RGD or ATWLPPR monomers. The improved targeting and localization exhibited by the RGD-ATWLPPR heterodimer provide a foundation for further investigations of its applicability in clinical tumor imaging. 相似文献3.
Alexander Zheleznyak Thaddeus J. Wadas Christopher D. Sherman Jessica M. Wilson Paul J. Kostenuik Katherine N. Weilbaecher Carolyn J. Anderson 《Molecular imaging and biology》2012,14(4):500-508
Purpose
The goal of this study was to determine the specificity of 64Cu-CB-TE2A-c(RGDyK) (64Cu-RGD) for osteoclast-related diseases, such as Paget's disease or rheumatoid arthritis.Procedures
C57BL/6 mice were treated systemically with osteoprotegerin (OPG) for 15?days or RANKL for 11?days to suppress and stimulate osteoclastogenesis, respectively. The mice were then imaged by positron emission tomography/computed tomography using 64Cu-RGD, followed by determination of serum TRAP5b and bone histology. Standard uptake values were determined to quantify 64Cu-RGD in bones and other tissues.Results
Mice treated with OPG showed decreased bone uptake of 64Cu-RGD at 1, 2, and 24?h post-injection of the tracer (p?<?0.01 for all time points) compared to vehicle controls, which correlated with a post-treatment decrease in serum TRAP5b. In contrast, mice treated with RANKL showed significantly increased bone uptake at 2?h post-injection of 64Cu-RGD (p?<?0.05) compared to the vehicle control group, corresponding to increased serum TRAP5b and OC numbers as determined by bone histology.Conclusions
These data demonstrate that 64Cu-RGD localizes to areas in bone with increased osteoclast numbers and support the use of 64Cu-RGD as an imaging biomarker for osteoclast number that could be used to monitor osteoclast-related pathologies and their treatments. 相似文献4.
Zhenying Chen Fangmeng Fu Fang Li Zhaohui Zhu Yinghong Yang Xiangjin Chen Bing Jia Shan Zheng Chao Huang Weibing Miao 《Molecular imaging and biology》2018,20(5):846-856