Expression of Human Vascular Endothelial Growth Factor Receptor Flt-1 Extracellular Domain 1-3 Loop cDNA in Pichia pastoris, Purification of the Expressed Product and Detection of Its Biological Activity

ＩＮＴＲＯＤＵＣＴＩＯＮＶａｓｃｕｌａｒｅｎｄｏｔｈｅｌｉａｌｇｒｏｗｔｈｆａｃｔｏｒ (ＶＥＧＦ)ｒｅｃｅｐｔｏｒＦｌｔ 1ｉｓａｋｉｎｄｏｆｇｌｕｃｏｐｒｏｔｅｉｎｗｉｔｈａｍｏｌｅｃｕｌａｒｗｅｉｇｈｔｏｆ 1 80ＫＤ .Ａｓａｆａｍｉｌｙｍｅｍｂｅｒｏｆｔｙｒｏｓｉｎｅｐｒｏｔｅｉｎｋｉｎａｓｅｒｅｃｅｐｔｏｒｓｕｂ ｔｙｐｅⅢ ,ｉｔｃｏｎｓｉｓｔｓｏｆｅｘｔｒａｃｅｌｌｕｌａｒ,ｔｒａｎｓｍｅｍｂｒａｎｅａｎｄｉｎｔｒａｃｅｌｌｕｌａｒｄｏｍａｉｎｓ.ＴｈｅｒｅａｒｅｓｅｖｅｎＩｇ ｌｉｋｅｌｏｏｐ…     

Acting via A_2 receptors，adenosine inhibits H_2O_2 production and elastase releaseon FMLP一stimulated

ＡｃｔｉｎｇｖｉａＡ２ｒｅｃｅｐｔｏｒｓ，ａｄｅｎｏｓｉｎｅｉｎｈｉｂｉｔｓＨ２Ｏ２ｐｒｏｄｕｃｔｉｏｎａｎｄｅｌａｓｔａｓｅｒｅｌｅａｓｅｏｎＦＭＬＰ一ｓｔｉｍｕｌａｔｅｄｎｅｕｔｒｏｐｈｉｌｓＺｈａｎｇＹｕ，ＢａＴｕ（ＤｅｐａｒｔｍｅｎｔｏｆＣａ...     

Propofol combined with diazepam synergistically potentiates the GABA-activated chloride current in rat sensory neurons

γ ａｍｉｎｏｂｕｔｙｒｉｃ ａｃｉｄＡ(ＧＡＢＡＡ)ｒｅｃｅｐｔｏｒｓｉｎｔｈｅｃｅｎｔｒａｌｎｅｒｖｏｕｓｓｙｓｔｅｍ (ＣＮＳ)ａｒｅｔｈｏｕｇｈｔｔｏｂｅａｐｏｔｅｎｔｉａｌｔａｒｇｅｔｓｉｔｅｏｆａｃｔｉｏｎｆｏｒａｖａｒｉｅｔｙｏｆｇｅｎｅｒａｌａｎｅｓｔｈｅｔｉｃｓ 1,2 Ｅｘｔｅｎｓｉｖｅｓｔｕｄｉｅｓｈａｖｅｓｈｏｗｎｔｈａｔｂａｒｂｉｔｕｒａｔｅｓ,ｂｅｎｚｏｄｉａｚｅｐｉｎｅｓ ,ｐｒｏｐｏｆｏｌ,ａｎｄｖｏｌａｔｉｌｅａｎｅｓｔｈｅｔｉｃｓｓｕｃｈａｓｈａｌｏｔｈａｎｅ ,ｉｓｏｆｌｕｒ…     

Relationship between the Mutation of IRS-1 Gene and β_3-adrenergic Receptor Gene

Ｏｂｅｓｉｔｙｉｓａｓｓｏｃｉａｔｅｄｗｉｔｈｉｎｓｕｌｉｎｒｅｓｉｓ ｔａｎｃｅ (ＩＲ) ,ｗｈｉｃｈｐｌａｙｓａｃｒｉｔｉｃａｌｒｏｌｅｉｎｔｈｅｄｅｖｅｌｏｐｍｅｎｔｏｆｔｙｐｅ 2ｄｉａｂｅｔｅｓｍｅｌｌｉｔｕｓ.ＴｈｅｍｅｃｈａｎｉｓｍｏｆＩＲｉｓｒｅｌａｔｅｄｔｏｔｈｅａｂｎｏｒｍａｌｉｔｉｅｓｏｆｉｎｓｕｌｉｎｒｅｃｅｐｔｏｒ,ｐｒｅ ｒｅｃｅｐｔｏｒ,ｐｏｓｔ ｒｅｃｅｐ ｔｏｒ ,ｒｅｓｕｌｔｉｎｇｉｎｄｅｆｅｃｔｉｖｅｍｅｔａｂｏｌｉｃｐａｔｈｗａｙｏｆｇｌｕｃｏｓｅ,ｌｉｐｉｄａｎｄ ｐｒ…     

Muscarinic stimulation rescues β_1-adrenergic response in transgenic mouse heart overexpressing β_2-adrenoceptor

ＩＮＴＲＯＤＵＣＴＩＯＮＩｎａｄｄｉｔｉｏｎｔｏｔｒａｄｉｔｉｏｎａｌｐｈａｒｍａｃｏｌｏｇｉｃａｌａｐｐｒｏａｃｈ ｅｓ ,ｍｏｕｓｅｇｅｎｏｍｉｃｍａｎｉｐｕｌａｔｉｏｎｈａｓｏｐｅｎｅｄｎｅｗａｖｅｎｕｅｓｔｏｉｎｖｅｓｔｉｇａｔｉｏｎｏｆｔｒａｎｓｍｅｍｂｒａｎｅｓｉｇｎａｌｔｒａｎｓｄｕｃｔｉｏｎ ,ｐａｒ ｔｉｃｕｌａｒｌｙｔｈｏｓｅｍｅｄｉａｔｅｄｂｙＧｐｒｏｔｅｉｎ ｃｏｕｐｌｅｄｒｅｃｅｐｔｏｒｓ .Ｏｗｉｎｇｔｏｔｈｅｃｒｉｔｉｃａｌｒｏｌｅｏｆβ ａｄｒｅｎｅｒｇｉｃｒｅｃｅｐｔｏｒ…     

DYNAMIC CHANGES OF SERUM VASCULAR ENDOTHELIAL GROWTH FACTOR LEVELS IN A RAT MYOCARDIAL INFARCTION MODEL

ＩＮＴＲＯＤＵＣＴＩＯＮ　　Ｖａｓｃｕｌａｒｅｎｄｏｔｈｅｌｉａｌｇｒｏｗｔｈｆａｃｔｏｒ(ＶＥＧＦ) ,ａｈｏｍｏｄ ｉｍｅｒｉｃ 34 ｔｏ 46 ｋＤａｈｅｐａｒｉｎ ｂｉｎｄｉｎｇｇｌｙｃｏｐｒｏｔｅｉｎ ,ｉｓａｐｏ ｔｅｎｔｓｔｉｍｕｌａｔｏｒｏｆｅｎｄｏｔｈｅｌｉａｌｃｅｌｌｒｅｐｌｉｃａｔｉｏｎａｎｄｉｓｒｅｇａｒｄｅｄａｓｏｎｅｏｆｔｈｅｍｏｓｔｓｐｅｃｉｆｉｃｏｆｔｈｅｋｎｏｗｎａｎｇｉｏｇｅｎｉｃｍｅｄｉａｔｏｒｓｂｅｃａｕｓｅｔｈｅｌｏｃａｌｉｚａｔｉｏｎｏｆｉｔｓｒｅｃｅｐｔｏｒｓｉ…     

ABSTRACTS FROM OTHER JOURNALS

ＩｍｕｎｏｇｅｎｉｃｉｔｙｏｆＧｅｌａｔｉｎＭｉｃｒｏｓｐｈｅｒｅｓＥｎｃａｐｓｕｌａｔｅｄＨｅｐａｔｉｔｉｓＢＶａｃｃｉｎｅｉｎＡｎｉｍａｌＴｒｉａｌ　ＺＨＡＯＪｉｎｇａｎｄＷＡＮＧＬｉｘｉｎ .ＮａｔｉｏｎａｌＶａｃｃｉｎｅ＆ＳｅｒｕｍＩｎｓｔｉｔｕｔｅ,Ｂｅｉｊｉｎｇ ,Ｐ .Ｒ .Ｃｈｉｎａ　　Ｏｂｊｅｃｔｉｖｅ　Ｔｏｒｅｓｅａｒｃｈａｎｄｏｐｔｉｍｉｚｅｔｈｅｆｏｒｍｕｌａｔｉｏｎｓｏｆｔｈｅｍｉｃｒｏｓｐｈｅｒｅｓｅｎｃａｐｓｕｌａｔｅｄｈｅｐ ａｔｉｔｉｓＢｖａｃｃｉｎｅ,ａｎｄｉｔｓｓｔａ…     

Evaluation of cardiac beta-adrenergic receptor function in children by dobutamine stress echocardiography

ＯｂｊｅｃｔｉｖｅＴｏｉｎｖｅｓｔｉｇａｔｅｔｈｅｒｏｌｅｏｆｄｏｂｕｔａｍｉｎｅｓｔｒｅｅｅｃｈｏｃａｒｄｉｏｇｒａｐｈｙｉｎｅｖａｌｕａｔｉｎｇｃａｒｄｉａｃｂｅｔａａｄｒｅｎｅｒｇｉｃｒｅｃｅｐｔｏｒ（βＡＲ）ｆｕｎｃｔｉｏｎａｎｄｒｅｓｐ...     

Application of Ambulatory Blood Pressure Monitoring in Essential Hypertension with Target-Organ Damage

Ｔｈｅｐｕｒｐｏｓｅｏｆｔｈｉｓｓｔｕｄｙｗａｓｔｏｄｅｔｅｒｍｉｎｅｗｈｅｔｈｅｒｔｈｉｓｎｏｒｍａｌａｍｂｕｌａｔｏｒｙｂｌｏｏｄｐｒｅｓｓｕｒｅ(ＡＢＰ)ｃｒｉｔｅｒｉｏｎｄｅｔｅｒ ｍｉｎｅｄｂｙＪＶＣ ＶＩｗａｓｓｕｉｔａｂｌｅｆｏｒＣｈｉｎｅｓｅｐａｔｉｅｎｔｓｗｉｔｈｅｓ ｓｅｎｔｉａｌｈｙｐｅｒｔｅｎｔｉｏｎ (ＥＨ) ,ｉｎｏｒｄｅｒｔｏｄｅｃｒｅａｓｅｔａｒｇｅｔｏｒｇａｎｄａｍａｇｅ (ＴＯＤ) .2 4 ｈｏｕｒＡＢＰｍｏｎｉｔｏｒｉｎｇ (ＡＢＰＭ)ｒｅｓｕｌｔｓｉｎ 132 5ｃａｓｅｓｗｉｔｈ…     

Effects of Black Tea Extract on Transplantable and Solid Tumors in Swiss Albino Mice

ＩＮＴＲＯＤＵＣＴＩＯＮＣａｎｃｅｒｈａｓｂｅｃｏｍｅｏｎｅｏｆｔｈｅｍｏｓｔｄｒｅａｄｅｄｄｉｓｅａｓｅｓｉｎｍｏｄｅｒｎｉｎｄｕｓｔｒｉａｌｉｚｅｄｗｏｒｌｄ .Ｄｕｅｔｏｔｈｉｓ,ｕｓｅｏｆｃｈｅｍｉｃａｌｓｏｆｎａｔｕｒａｌｏｒｂｉｏｌｏｇｉｃａｌｏｒｉｇｉｎａｓｃａｎｃｅｒｃｈｅｍｏｐｒｅｖｅｎｔｉｖｅａｇｅｎｔｓｈａｓｒｅｃｅｉｖｅｄａｇｒｅａｔｄｅａｌｏｆｅｍｐｈａｓｉｓａｓａｎｏｖｅｌａｐｐｒｏａｃｈｔｏｃａｎｃｅｒｃｏｎｔｒｏｌ (Ａｍｅｓ,1 983;Ａｍｅｓ,ＧｏｌｄａｎｄＷｉｌｌｅｔ…     

非洲爪蟾卵母细胞内源性及外源性ATP和GABA受体介导的膜反应比较

目的 :对非洲爪蟾卵母细胞表达的ATP和GABA激活电流进行比较。方法 :包括mRNA和cDNA的提取、卵母细胞的微量注射及双电极电压钳技术。结果 :①在表达P2X2 或P2X4 的卵母细胞上 ,外加ATP可分别诱导一被Zn2 + 增强的内向电流。②在表达鸡脑mRNA的卵母细胞上 ,可记录到GABA激活电流 ,且此电流可被bicucul line(一种GABAA 受体选择拮抗剂 )所阻断。③在表达了人视网膜 ρ1亚基cRNA的卵母细胞上 ,外加GABA可引起一不被bicuculline阻断的内向电流 ,后者可被I4AA(一种GABAC 受体特异性拮抗剂 )阻断。④在具有滤泡膜的卵母细胞上可记录到 3种形式的ATP激活电流和一外向的慢的GABA激活电流。⑤在除去滤泡膜的卵母细胞上均未记录到ATP和GABA激活电流。结论 :卵母细胞上存在内源性的嘌呤受体、GABAB 和GABAC 受体 ;且此内源性受体存在于滤泡膜上 ;P2X2 、P2X4 嘌呤受体及GABAA 和GABAC 受体均可在卵母细胞上表达 ,表达的受体所介导的激活电流与内源性受体介导的激活电流有明显区别     

卵母细胞基因表达系统的应用研究

目的 :建立研究神经生理及药理的卵母细胞表达模型 ,并对鸡脑mRNA在卵母细胞上表达的氨基酸类受体进行研究。方法 :采用盐酸胍或异硫氰酸胍法抽提总RNA ,经寡聚脱氧胸苷 (oligo -dT)纤维素柱纯化得mRNA。mRNA微注射到卵母细胞内进行表达 ,以双电极电压钳技术研究表达的受体。结果 :模型能明显表达外源性mRNA表达的受体 ,而阴性对照无表达。在卵母细胞膜上表达的γ -氨基丁酸 (γ -aminobutyricacid ,GABA) ,甘氨酸(Glycine ,Gly)及红藻氨酸 (Kainate ,KA)受体 ,施加GABA ,Gly及KA均能引起一剂量依赖的内向电流 ,相应的拮抗剂可阻断其电流。剂量效应曲线显示GABA及KA诱导电流的半有效浓度 (EC5 0 )分别为 2 .9× 10 -5mol/L及 6 .3× 10 -5mol/L。结论 :卵母细胞可明显表达鸡脑mRNA编码的KA ,Gly及GABA受体 ,其药理特性与天然受体相似 ,是一个良好的神经生理及药理研究模型。     

Zn~(2+)对鸡脑mRNA表达在卵母细胞膜上的GABA及KA受体的调制作用

目的：探讨Ｚｎ２＋和氨基酸类递质之间是否存在内在关系或相互作用。方法：实验以盐酸胍方法抽提鸡脑ｍＲＮＡ，通过微量注射把信使核糖酸（ｍＲＮＡ）导入卵母细胞，利用卵母细胞的基因表达系统进行表达。应用双电极电压钳方法研究所表达的受体。结果：（１）γ－氨基丁酸（ＧＡＢＡ）及红藻氨酸（ＫＡ）受体在卵母细胞膜上表达明显。施加相应的激动剂均能引起一剂量依赖的内向电流，其幅值分别高达２９４±６．４ｎＡ和３０９．５±４．９ｎＡ，相应的拮抗剂荷包牡丹碱（ｂｉｃｕｃｕｌｉｎｅ）和６－氰基－７－硝基喹恶啉土卫四（ＣＮＯＸ）可阻断其电流，而阴性对照无此现象。（２）剂量效应曲线显示Ｚｎ２＋的浓度为３０ｍｍｏｌ／Ｌ时开始抑制３００ｍｍｏｌ／Ｌ的ＫＡ诱导电流。半抑制浓度（ＩＣ５０）为７１０ｍｍｏｌ／Ｌ。（３）Ｚｎ２＋在ｎｍｏｌ／Ｌ级浓度时即对ＧＡＢＡ诱导电流有抑制作用，ＩＣ５０为２５ｍｍｏｌ／Ｌ。（４）Ｚｎ２＋的这种抑制作用是可逆的，即去除灌流液中的Ｚｎ２＋后，ＫＡ及ＧＡＢＡ诱导电流恢复到原来大小。结论：Ｚｎ２＋可能作为一种神经调制物通过非竞争性抑制来调制ＧＡＢＡ及ＫＡ受体。     

A single amino acid change within the ion-channel domain of the gamma-aminobutyric acid rho1 receptor accelerates desensitization and increases taurine agonism

BACKGROUND: GABAC receptors are part of the ligand-gated ion channel family of receptors that share some functional and structural features: e.g., they have four putative transmembrane domains (TM1-TM4) and the TM2-segment is presumed to form the ion-channel. GABAC receptors open chloride channels and do not desensitize even after long exposures to GABA. These receptors are highly expressed in vertebrate retina, where they may play a unique role due to their unusual biophysical and pharmacologic characteristics. METHODS: To determine whether the TM2 domain plays a role in the process of desensitization of GABAC receptors, we used site-directed mutagenesis to produce several permutations within the leucine (L9') residue of the TM2 domain of the human GABArho1 subunit. Recombinant receptors were expressed in Xenopus laevis oocytes and their functional and pharmacologic properties were studied by using a two-microelectrode, voltage-clamp. RESULTS: Several amino acid changes led to receptors that did not generate GABA-currents, whereas an Asp for Leu mutation in the well-conserved L9' position of the rho1 subunit (L301D-rho1) generated a fast-desensitizing, bicuculline-resistant receptor that was antagonized by TPMPA, a specific GABAC receptor antagonist. Moreover, in contrast with wild-type rho1 receptors, which are practically not gated by taurine, L301D-rho1 mutant receptors generated substantial taurine-currents. CONCLUSIONS: Substitution of L9' residue in the TM2 region of GABArho1 receptor for an amino acid residue with an acidic lateral chain greatly accelerates its desensitization rate and increases taurine-agonism. This mutant will be useful to study mechanisms involved in gating and desensitization of GABAC receptors in particular, and of neurotransmitter receptors in general.     

Effects of free radicals and amyloid β protein on the currents of expressed rat receptors in Xenopus oocytes

Objective To investigate the effects of free radicals (FRs) and amyloid β protein 1-40 ( Aβ(1-40)) on the functions of expressed neurotransmitter receptors (NRs) i n Xenopus oocytes.Methods Total RNA and messenger RNA (mRNA) was prepared from 3-month-old Wistar rat br ain tissues with Promega kits and microinjected into maturated Xenopus oocyt es (stages Ⅴ-Ⅵ) with 50 nl (50 ng) for each oocyte. The microinjec ted oocy tes were incubated with modifiedBath’s solution at 19.0℃±1.0℃ for receptor expression and their currents were recorded with double electrode voltage clamp technique. Superoxide anion free radicals (SAFRs) were produced via a reaction system (HPXXO) with hypoxanthine (HPX, 0.05 mol/L) and xanthine oxidase (XO, 0.1 U/L). In order to observe the effects of Aβ and SAFRs on the e xpressed glutamate receptor, HPX/XO and Aβ(1-40) were added to incubation solution at 12 h, 24 h and 96 h before recording.Results The results showed that the oocytes expressed functional NRs originating from ra t brain tissues. These NRs included muscarinic acetylcholine (mACh), glutamate (Glu), dopamine (DA), serotonin (5-HT) and γ-aminobutyric acid (GABA). T he c urrent characteristics of expressed receptors were inward currents carried by ch loride ion with their equibrilium potentials close to -22 mV. The extent of ef fect on the current of expressed glutamate receptor from rat brain was different among different Aβ concentrations and incubation times. Aβ(1-40) at a c oncentration of 20 nmol/L had little effect on the currents of expressed rat br ain glutamate receptors up to 24 h of incubation period; but the currents of gl utamate receptor were significantly decreased (25% off, P<0.01) in the trea tment of 60 nmol/L Aβ(1-40) over 24 h. Moreover, when 20 nmol/L Aβ (1-40) was co-incubated over 12 h with SAFRs produced by the reaction syste m of HPXXO, it was found that the currents of expressed rat bra in glutamate rec eptors had been changed markedly. When the oocytes were co-treated with 60 nm ol/L Aβ(1-40) and SAFRs over a period of 12 h, the currents of glutamate receptor significantly decreased (21% off, P<0.05), and the decreased perce ntage reached 52% over 24 h co-treatment with 60 nmol/L Aβ(1-40) and SA FRs. In addition, vitamin E had a partial effect against this inhi bitory effect.Conclusion The results suggest that Aβ has a kind of inhibitory effect upon the current of the glutamate receptor, similar to the effects of free radicals. The effects c an be antagonized by vitamin E. These imply that Aβ may play a role via inhibi ting receptor function in the pathophysiology of Alzheimer’s disease.     

丙戊酸钠对大鼠脑NMDA受体在爪蟾卵母细胞表达的影响

目的 :观察丙戊酸钠对大鼠脑 N-甲基 - D-天冬氨酸 ( NMDA)受体在非洲爪蟾卵母细胞表达的影响。方法 :采用电压钳位技术记录表达于非洲爪蟾卵母细胞膜上的大鼠脑 NMDA受体通道电流及特性并观察 0 .1和 0 .5 mmol· L-1 丙戊酸钠对大鼠脑 NMDA受体通道电流的影响。结果 :丙戊酸钠 ( 0 .1和 0 .5 mmol· L-1 )对表达于非洲爪蟾卵母细胞的大鼠脑 NMDA受体功能有抑制作用 ,电流峰值由 ( 97.5± 9.8) n A降至 ( 79.7± 1 0 .5 ) n A、( 61 .3± 1 2 .5 ) n A ( P<0 .0 5和P<0 .0 1 )。结论 :丙戊酸钠对大鼠脑 NMDA受体有明显的抑制作用     

Ion-exchange chromatography (IC) in the determination of serum transition metals and its clinical application.

The reproducibility of ion-exchange chromatography (IC) (Dionex Bio-LC) in measuring the serum transition metals Cu and Zn was found to be fairly good (CV = +/- 2.1% and +/- 2.7% within runs, and +/- 2.5% and +/- 4.3% among runs for Cu and Zn, respectively). For clinical application, sera from 20 normal volunteers and 20 patients with cancers of the digestive tract (10 early and 10 late stage cases) were analyzed. The means of serum Cu (15.54 +/- 0.31 mumol/L) and serum Zn (14.84 +/- 0.31 mumol/L) in normal volunteers were very close to the values previously determined by atomic absorption spectrophotometry in our laboratory. In the group of late stage cancer patients, the means of serum Cu (24.18 +/- 0.63 mumol/L) and the Cu/Zn ratio (1.97 +/- 0.07) were significantly elevated (P < 0.01), while serum Zn (13.01 +/- 0.61 mumol/L) was remarkably decreased (P < 0.05). There were no significant changes of serum Cu, Zn and Cu/Zn ratio in early stage cancer patients. It is concluded that the IC method is rapid, accurate, sensitive, relatively simple and could be useful in diagnosing late stage cancers of the digestive tract.     

乳腺癌患者血清微量元素测定的临床价值

目的:探讨9种血清微量元素与乳腺癌的关系。方法:采用原子吸收分光光谱法对61例健康妇女、78例乳腺癌手术前及61例乳腺癌手术后血清中铜(Cu)、铁(Fe)、锌(Zn)、锰(Mn)、铅(Pb)、铬(Cr)、镉(Cd)、镍(Ni)、钼(Mo)9种微量元素的含量进行测定。结果:3组血清中的微量元素含量有显著差异,其中血清Fe、Cu、Zn、Mn含量乳癌组明显高于健康对照组,血清Fe、Zn、Cu、Ni含量乳腺癌术前组明显高于乳腺癌术后组(P<0.01),血清Cu/Zn比值乳腺癌组高于健康对照组。结论:结果提示Fe、Zn、Cu、Mn、Ni可能是乳癌的危险因素,Mo可能为乳癌的保护因素。     

GABA受体对谷氨酸受体的调控机制研究

目的 研究γ-氨基丁酸（GABA）受体对离子型谷氨酸受体的调控机制。方法 采用细胞膜片钳电生理技术,以GABA、谷氨酸（Glu）刺激记录GABA受体及Glu受体离子通道介导的全细胞电流（IGABA,IGlu）,观察GABA对谷氨酸受体离子通道活性的调控;并以荷包牡丹碱（Bic）抑制IGABA、以SO2-4或葡萄糖酸根（gluconate-）代替Cl-,研究其抑制作用的分子机制。结果 相同浓度GABA与Glu共给药诱导IGABA+Glu较单独给药诱导电流IGABA与IGlu之和小,该差异随浓度升高而降低;不同浓度GABA对Glu 30μmol/L诱导电流IGlu抑制作用随GABA浓度升高而增强,该抑制作用可以被Bic、SO2-4与gluconate-消除。结论 GABAA受体能抑制谷氨酸受体介导的全细胞电流,该作用具有浓度依赖性,其抑制机制可能与Cl-的通透性有关。     

铝致Alzheimer病对尿中微量元素排出的影响

目的：探讨Alzheimer病时，对微量元素铜、锌、锰、硒、镉等在尿中排出的影响。方法：雌性健康SD大鼠29只，随机分为卵巢假切组（sham组）、卵巢切除组（ovx组）、卵巢切除合并慢性铝中毒组（ovx＋AL^3＋组）、卵巢切除合并慢性铝中毒及尼尔雌醇灌胃组（ovx＋＋AL^3＋＋E2组）。收集24的尿液，采用日本岛津ICPQ—1012型电感耦合等离子体发射光谱仪（ICP—AES）测定尿中微量元素含量。结果：卵巢切除合并慢性铝中毒及尼尔雌醇灌胃组（ovx＋＋AL^3＋＋E2组）分别与卵巢假切组比较尿铝（P〈0．01）、尿镁（P〈0．05）、尿硒（P〈0．01）、尿钙（P〈0．01）、尿镉（P〈0．05）排出明显增加；铜、锌、锰排出量与卵巢假切组相比均无统计学意义（P〉0．05）。结论：铝致Alzheimer病对尿中铝、镁、硒、钙和镉的排出明显增加，而对铜、锌、锰在尿中的排出无影响。