Relationship between trefoil factor 1 expression and gastric mucosa injuries and gastric cancer

AIM: To determine whether trefoil factor 1 (TFF1) is associated with mucosa healing and carcinoma suppression, we assess the expression of trefoil factor 1 in normal and pathologic gastric mucosa. METHODS: TFF1 in normal and pathologic gastric mucosa was assessed by immunohistochemical method, and the average positive A was estimated by Motic Images Advanced 3.0 software. RESULTS: Increased TFF1 was detected in gastritis, gastric ulcer and duodenal ulcer compared with normal mucosa. The same result could be seen in multiple and compound ulcer compared with simple ulcer. There was no significant difference between gastric ulcer and duodenal ulcer, gastritis and simple ulcer respectively. Increased TFF1 was detected in the peripheral mucosa of the gastric adenocarcinoma compared with normal mucosa. The expression of TFF1 in gastric adenocarcinoma was related to the differentiation of adenocarcinoma. The lower the differentiation of adenocarcinoma, the weaker the expression of TFF1. There was no TFF1 expressed in low-differentiated adenocarcinoma. The expression of TFF1 in middle and highly differentiated adenocarcinoma was a little lower than that in normal mucosa. But there was no significant difference. No TFF1 was assessed in esophageal squamous carcinoma and peripheral tissue. There was no significant difference between male and female. CONCLUSION: The expression of TFF1 was higher in gastritis and peptic ulcer than that in normal mucosa, and was also higher in multiple and compound ulcer than in simple ulcer. It seems that TFF1 plays a role in gastric mucosa protection and epithelial restitution. Increased expression of TFF1 in peripheral tissue suggests that TFF1 is associated with mechanism of carcinoma suppression and differentiation. Decreased expression of TFF1 in carcinoma and its relativity to the differentiation suggests that TFF1 is related to gland and cell destruction of carcinoma.     

三叶因子1表达与胃黏膜损伤及胃癌的关系

目的　测定三叶因子 1(TFF1)在正常及病理条件下胃黏膜中的表达情况 ,探讨TFF1在胃黏膜损伤修复及胃癌抑制中的作用及意义。方法　应用免疫组化方法测定正常及不同病理条件下胃黏膜中TFF1的表达情况 ,通过图像分析软件分析阳性信号平均吸光度值以了解其表达情况。结果　胃炎、胃溃疡及十二指肠球部溃疡患者TFF1表达明显高于正常胃黏膜 (0 .5 1± 0 .0 5 ,0 .5 1± 0 .0 6 ,0 .5 0± 0 .0 6比 0 .4 4± 0 .0 6 ;P值均 <0 .0 1)。胃腺癌患者癌旁组织表达 (0 .5 1± 0 .0 7)明显高于正常胃黏膜 ,而腺癌组织的表达强度则与癌组织的分化程度呈正比 ,分化程度愈低 ,表达愈弱 ,低分化腺癌无阳性表达 ,中、高分化腺癌表达 (0 .4 1± 0 .0 7)略低于正常黏膜 ,但差异无显著性 (P >0 .0 5 )。结论　TFF1表达随黏膜损伤程度的加重而表达增强 ,提示其在胃黏膜保护及促进上皮重建机制中具有一定的作用。TFF1在癌旁组织中表达增强提示其可能与肿瘤抑制及分化机制有关 ,而在癌组织中表达减弱可能与其分泌减少有关。     

Relationship between trefoil factor 1 expression and gastric rnucosa injuries and gastric cancer

AIM: To determine whether trefoil factor 1 (TFF1) is associated with mucosa healing and carcinoma suppression, we assess the expression of trefoil factor 1 in normal and pathologic gastric mucosa.METHODS: TFF1 in normal and pathologic gastric mucosa was assessed by immunohistochemical method, and the average positive A was estimated by Motic Images Advanced 3.0 software.RESULTS: Increased TFF1 was detected in gastritis, gastric ulcer and duodenal ulcer compared with normal mucosa. The same result could be seen in multiple and compound ulcer compared with simple ulcer. There was no significant difference between gastric ulcer and duodenal ulcer, gastritis and simple ulcer respectively. Increased TFF1 was detected in the peripheral mucosa of the gastric adenocarcinoma compared with normalmucosa. The expression of TFF1 in gastric adenocarcinoma was related to the differentiation of adenocarcinoma. The lower the differentiation of adenocarcinoma, the weaker the expression of TFF1. There was no TFF1 expressed in low-differentiated adenocarcinoma. The expression of TFF1 in middle and highly differentiated adenocarcinoma was a little lower than that in normal mucosa. But there was no significant difference. No TFF1 was assessed in esophageal squamous carcinoma and peripheral tissue. There was no significant difference between male and female.CONCLUSION: The expression of TFF1 was higher in gastritis and peptic ulcer than that in normal mucosa, and was also higher in multiple and compound ulcer than in simple ulcer. It seems that TFF1 plays a role in gastric mucosa protection and epithelial restitution. Increased expression of TFF1 in peripheral tissue suggests that TFFLis associated with mechanism of carcinoma suppression and differentiation. Decreased expression of TFF1 in carcinoma and its relativity to the differentiation suggests that TFF1 is related to gland and cell destruction of carcinoma.     

三叶因子Ⅰ和Ⅱ在胃癌和癌前状态中的表达

目的　探讨三叶因子Ⅰ (trefoilfactor1,TFF1)和Ⅱ (TFF2 )与癌前状态及胃癌发生及发展的关系。方法　对 14 0例经病理证实的不同胃黏膜病变 ,采用免疫组化方法进行TFF1、TFF2蛋白的定位和半定量检测。结果　在慢性浅表性胃炎、胃溃疡、慢性萎缩性胃炎和胃癌 4种病变中 ,TFF1表达呈逐渐减弱趋势 (P <0 0 5)。TFF2在上述 4种病变中的表达差异亦有显著性 (P <0 0 5) ,其中在胃溃疡、慢性萎缩性胃炎和胃癌组织中 ,TFF2表达逐渐降低 (P <0 0 5)。结论　TFF1和TFF2蛋白在癌前状态和胃癌的表达降低 ,TFF1和TFF2的作用机制以及临床应用前景有待于进一步研究     

三叶因子2表达与胃溃疡易感性的相关研究

目的　三叶因子 (trefoilfactor)是一组新发现的对胃肠道有保护作用的多肽类物质 ,通过对三叶因子 2 (TFF2 )mRNA在胃溃疡与浅表性胃炎患者胃黏膜中表达水平的差异研究 ,探讨其在胃溃疡发病机制中的作用。方法　胃镜下钳取 32例胃溃疡内侧缘、溃疡远处和 36例浅表性胃炎患者的组织标本 ,利用原位杂交方法分别对不同组织中TFF2mRNA进行定位和半定量测定 ,比较TFF2mRNA在各组中的表达差异。结果　TFF2mRNA主要分布于胃小凹基底部的柱状细胞、幽门腺腺泡及导管细胞中。TFF2mRNA在浅表性胃炎组的表达高于胃溃疡内侧缘组 ,在胃溃疡内侧缘表达要高于溃疡 5cm外的胃黏膜 ,但组间表达差异无显著性 (P均 >0 .0 5 ) ,而浅表性胃炎标本TFF2mRNA阳性程度显著高于溃疡外 5cm组 (P <0 .0 5 )。结论　TFF2mRNA在胃黏膜中广泛分布 ,胃溃疡患者胃黏膜中TFF2mRNA的低表达可能是胃溃疡易患因素之一。     

三叶肽对胃粘膜保护机理的研究进展

三叶肽家族是一类较新的、对胃粘膜有保护作用的因子，主要由乳癌相关肽、解痉多肽和肠三叶因子组成，其特征是均由6个半胱氨酸残基借3个二硫键连接形成，呈三叶状结构。三叶肽对粘膜的保护作用，可能在于增强受损粘膜周围完好的上皮细胞向粘膜损伤表面迁移覆盖，或与粘液中的糖蛋白相互作用，加强粘液凝胶层，抵抗粘膜表层有害物质的损伤等。三叶肽的研究可能对治疗胃十指肠溃疡与修复开辟了新途径。     

Expression of trefoil factors 1 and 2 in precancerous condition and gastric cancer

AIM: To study the expression of trefoil factor 1 (TFF1) and TFF2 in precancerous condition and gastric cancer and to explore the relationship between TFFs and tumorigenesis, precancerous condition and gastric cancer. METHODS: The expression of TFF1 and TFF2 was immunohistochemically analyzed in paraffin-embedded samples from 140 patients including 35 cases of chronic superficial gastritis (CSG), 35 cases of gastric ulcer (GU), 35 cases of chronic atrophic gastritis (CAG) and 35 cases of gastric cancer (GC). RESULTS: TFF1 and TFF2 were located in cytoplasm of gastric mucous cells. In CSG, GU, CAG and GC, the level of TFF1 expression had a decreased tendency (P<0.05). The expression of TFF2 was higher in GU than in CSG, but the difference was not significant. The expression of TFF2 also had a decreased tendency in GU, CAG, and GC (P<0.05). CONCLUSION: The reduced expression of TFF1 and TFF2 in precancerous conditions and gastric cancer may be associated with the proliferation and malignant transformation of gastric mucosa. More investigations are needed to explore the mechanism of TFFs and the relationship between TFFs and gastric cancer.     

Decreased expression of gastrokine 1 in gastric mucosa of gastric cancer patients

AIM: To investigate the expression of gastrokine 1(GKN1) in normal gastric mucosa, precancerous lesions and gastric cancer tissues, and to analyse its correlations with tumour site and pathological pattern.METHODS: Thirty gastric cancer patients(12 cases of diffuse type and 18 cases of intestinal type), 13 atrophic gastritis patients and 15 healthy volunteers with almost normal gastric mucosa(superficial gastritis) were enrolled in this study. Helicobacter pylori(H. pylori) infection was examined in all subjects. All gastric mucosa biopsy specimens were obtained. Cancer-adjacent specimens were taken from corresponding gastric cancer patients. Immunohistochemistry and real-time PCR were performed to determine the expressions of the GKN1 protein and m RNA, respectively.RESULTS: H. pylori infection had no significant association with age, gender, tumour site or pathological pattern in all subjects. Compared with the superficial gastritis and atrophic gastritis groups, the expression of GKN1 protein(P = 0.011) and m RNA(P < 0.001) in gastric cancer was significantly decreased. The GKN1 m RNA level in diffuse type gastric cancer was significantly lower than in intestinal type gastric cancer(0.296 ± 0.076 vs 0.525 ± 0.164, P < 0.001).CONCLUSION: Compared with almost normal gastric mucosa, GKN1 expression in the gastric mucosa of gastric cancer patients is decreased; this is associated with progression and prognosis of gastric cancer.     

三叶因子1的研究与进展

TFF1是三叶因子家族成员之一,在胃肠道黏膜屏障和修复中发挥着重要的生理功能.通过与黏蛋白结合对胃肠道起保护作用,并涉及黏膜重建的不同步骤,特别是调节细胞.细胞连接、细胞移行.近年研究发现,TFF1与细胞迁移、增殖、分化、血管形成等密切相关.他可以延迟细胞从G1期向S期转化,从而减少细胞凋亡,参与细胞内蛋白折叠而且与消化道疾病的发生密切相关.TFF1在胃肠道不同的部位发挥的作用不同,在胃组织中发挥肿瘤抑制因子的作用,但在结肠组织中却发挥着致癌因子的作用,促进结肠肿瘤的发生和发展,并且通过不同的信号途径发挥作用.本文主要对上述的新近研究进行综述.     

TFF1在功能性消化不良和慢性胃炎中的表达

目的 测定三叶因子1(trefoil factor1，TFFl)在正常、功能性消化不良、慢性胃炎胃粘膜中的表达情况，探讨TFF1与功能性消化不良和慢性胃炎的关系。方法 应用免疫组化方法测定正常人和功能性消化不良、慢性胃炎患者胃窦黏膜中TFF1的表达，通过图像分析软件分析其阳性信号平均光密度值。结果 慢性胃炎组TFFl的表达明显高于正常组和功能性消化不良组，而正常组和功能性消化不良组中表达均较弱，二者统计学上无差异。结论 TFFl在正常组和功能性消化不良组中表达相似，认为TFF1与功能性消化不良的发病无关：而慢性胃炎组表达较高，认为与TFFl在胃黏膜屏障功能保护和促进上皮重建的机制有关。     

小肠三叶肽在大肠癌中的表达及其意义

目的　探讨小肠三叶肽 (ITF)与大肠癌的发生、进展及预后的关系。方法　收集手术切除的大肠癌标本 ,同时取距癌灶 5cm以外的癌旁组织及 10cm以外的正常组织。用原位杂交法检测癌组织、癌旁组织及正常组织中ITFmRNA ,同时结合临床病理资料进行分析。结果　癌组织、癌旁组织及正常组织均有不同程度的ITFmRNA表达 ,三组间ITFmRNA表达比较 ,差异无显著性 (P >0 .0 5 )。在结肠癌组织中 ,Dukes分期较高 (B、C、D期 )组的ITFmRNA表达 (0 .3 14±0 .119)明显低于Dukes分期较低 (A期 )组的表达 (0 .45 3± 0 .10 2 ) ,两者比较差异有显著性 (P <0 .0 5 )。但在癌旁和正常组织中 ,ITFmRNA的表达在不同Dukes分期组中的表达差异无显著性 (P>0 .0 5 )。不同分化程度组间ITFmRNA的表达差异亦无显著性 (P >0 .0 5 )。ITFmRNA的表达与是否有淋巴结转移及远处转移无密切相关 (P >0 .0 5 )。结论　ITFmRNA不仅表达于正常结肠粘膜 ,亦表达于癌旁和癌组织。ITF的表达在Dukes分期较高组低于分期较低组 ,且与分化程度有潜在的正相关 ,提示ITF可能与肿瘤的进展呈负相关     

TFF2在胃癌、癌旁及正常胃黏膜组织中的表达及其与血管生成的关系

目的:探讨三叶因子2(TFF2)、血管内皮生长因子(VEGF)和微血管密度(MVD)在胃癌发生、发展、浸润和转移中的作用.方法:选取广西医科大学第一附属医院2008-01/2009-06接受胃大部切除术的胃癌标本50例,采用SP免疫组织化学方法检测30例正常胃黏膜组织、50例癌旁组织和50例胃癌组织中TFF2、VEGF...     

高海拔因素对人胃黏膜组织结构的影响及机制初探

目的 初步探讨西藏高海拔因素对人胃黏膜大体形态、组织结构的影响及其机制.方法 随机选择平均海拔为4500米的西藏山南地区错那镇患有慢性高原病(CMS)的世居藏族居民25例为研究组;选择平均海拔2000米以下的西藏山南地区勒布镇健康世居藏族居民25例为对照组,两组均行胃镜检查和胃黏膜活检,观察胃黏膜内镜下表现、组织病理学改变及组织超微结构特点;同时用免疫组化法检测胃黏膜组织中缺氧诱导因子1α(HIF-1α)表达.结果 研究组中,胃黏膜色泽较对照组而言呈暗红、深红;慢性胃体炎和胃溃疡的检出率在两组间差异有统计学意义(26.7%、6.1%比3.3%、0%,P<0.05),幽门收缩情况差异有统计学意义(33%比70%,P<0.05).研究组与对照组比较,非萎缩性胃体炎和慢性萎缩性胃窦炎检出率差异均有统计学意义(P<0.05).透射电镜观察发现,研究组胃黏膜上皮细胞受损.CMS患者胃黏膜组织中HIF-1α表达明显增高(102.9±34.04比14.04±6.53,P<0.05).结论 高海拔因素可能与胃黏膜损伤有关,CMS患者胃黏膜组织中HIF-1α基因的上调是高原胃黏膜病变的可能机制.     

慢性胃炎患者胃黏膜三叶因子1与细胞间黏附分子1蛋白表达

[目的]在幽门螺杆菌(Hp)阴性慢性浅表性胃炎(CSG)中探讨脾胃湿热证与三叶因子1(TFF1)、细胞间黏附分子1(ICAM-1)蛋白表达的相关性。[方法]Hp阴性CSG患者(脾胃湿热组27例,脾虚组10例)及对照组10例,经临床检查、胃镜取标本、病理学及免疫组化SP法检测胃黏膜炎症程度及TFF1、ICAM-1的蛋白表达情况。[结果]胃镜下脾胃湿热组胃黏膜充血水肿较脾虚证明显。炎症程度:脾胃湿热组>对照组(P<0.01),脾虚组>对照组(P<0.05),脾胃湿热组稍重于脾虚组。TFF1蛋白表达:脾胃湿热组>脾虚组>对照组。ICAM-1蛋白表达:脾胃湿热组>脾虚组稍高于对照组。ICAM-1蛋白表达与炎症程度呈正相关(P<0.01)。TFF1与ICAM-1蛋白表达呈正相关(P<0.01)。[结论]脾胃湿热证时TFF1、ICAM-1蛋白均呈高表达。提示脾胃湿热证中TFF1可能与机体正气抗邪状态有关;ICAM-1可能参与湿邪致病的分子机制。     

Evaluation of trefoil factor 3 as a non-invasive biomarker of gastric intestinal metaplasia and gastric cancer in a high-risk population

BackgroundAdenocarcinoma is preceded by chronic atrophic gastritis, gastric intestinal metaplasia and dysplasia. Trefoil factor 3 (TFF3) is a peptide secreted by goblet cells, which is abundantly present in intestinal metaplasia.AimTo evaluate the utility of serum TFF3 as a non-invasive biomarker for the diagnosis of intestinal metaplasia and gastric cancer.MethodsSingle-center, cross-sectional study of 274 patients who consecutively underwent upper gastrointestinal endoscopy with gastric biopsies (updated Sydney system). TFF3 levels were measured in serum by a commercial ELISA kit. Patients with normal histology or chronic atrophic gastritis without intestinal metaplasia comprised the control group. In addition, 14 patients with invasive gastric cancer were included as a reference group. The association between TFF3 levels and intestinal metaplasia was assessed by logistic regression.ResultsPatients with intestinal metaplasia (n = 110) had a higher median TFF3 level as compared to controls (n = 164), 13.1 vs. 11.9 ng/mL, respectively (p = 0.024). Multivariable logistic regression showed a no significant association between TFF3 levels and intestinal metaplasia (OR = 1.20; 95%CI: 0.87–1.65; p-trend = 0.273). The gastric cancer group had a median TFF3 level of 20.5 ng/mL, and a significant association was found (OR = 3.26; 95%CI: 1.29–8.27; p-trend = 0.013).ConclusionSerum levels of TFF3 do not discriminate intestinal metaplasia in this high-risk Latin American population. Nevertheless, we confirmed an association between TFF3 levels and invasive gastric cancer.     

三叶因子1与胃癌关系研究进展

三叶因子1(TFF1)属三叶因子家族,是近年来被人们注意到的具有胃肠道粘膜保护及修复作用的生长因子类小分子多肽物质。其基因现已被认为是一个重要的胃癌抑癌基因。现就三叶因子1的结构、功能、分布、表达及其与胃癌关系的研究进展作一综述。     

Nesfatin-1对离体胃黏膜细胞胃酸分泌的影响

目的:研究nesfatin-1对离体培养的大鼠胃黏膜酸分泌的影响,探讨nesfatin-1对H+/K+-ATP酶mRNA及蛋白表达的影响.方法:酶解法分离大鼠胃黏膜细胞,细胞免疫荧光检测法鉴定细胞.用不同浓度的nesfatin-1(10-4-10-1μmol/L)对胃黏膜细胞进行处理0、1、2、3、4h,设立空白对照组,以14C-氨基比林摄取为酸分泌指标,检测nesfatin-1对大鼠离体的胃黏膜细胞酸分泌的影响.用RT-PCR法及Western印迹法检测nesfatin-1对胃黏膜细胞H+/K+-ATP酶alpha(α)亚基和beta(β)亚基mRNA及蛋白表达的影响.结果:Nesfatin-1在10-1μmol/L浓度下,在1、2h能够抑制离体培养的大鼠胃黏膜细胞的酸分泌.Nesfatin-1(10-1μmol/L)在1、2、3h均能够抑制H+/K+-ATP酶α亚基mRNA表达水平;在1、2h能够抑制H+/K+-ATP酶β亚基mRNA表达水平,分别与对照组相比,差异有统计学意义(均P<0.01).Nesfatin-1(10-4-10-1μmol/L)作用胃黏膜细胞2h时,呈剂量依赖性抑制α亚基和β亚基mRNA表达水平,分别与对照组相比,差异有统计学意义(均P<0.01).Nesfatin-1(10-1μmol/L)在1、2、3h能够抑制α亚基蛋白表达水平;在2、3h能够抑制β亚基蛋白表达水平,分别与对照组相比,差异有统计学意义(均P<0.01).Nesfatin-1(10-3-10-1μmol/L)作用胃黏膜细胞2h时,呈剂量依赖性抑制α亚基和β亚基蛋白表达水平,与对照组相比,差异有统计学意义(均P<0.01).结论:Nesfatin-1能抑制离体培养的大鼠胃黏膜细胞的酸分泌,有可能是通过下调H+/K+-ATP酶α亚基和β亚基的mRNA及蛋白表达的水平影响酸分泌.     

Pretreatment with intestinal trefoil factor alleviates stress-induced gastric mucosal damage via Akt signaling

BACKGROUNDStress-related gastric mucosal damage or ulcer remains an unsolved issue for critically ill patients. Stress ulcer prophylaxis has been part of routine intensive care, but uncertainty and controversy still exist. Co-secreted with mucins, intestinal trefoil factor (ITF) is reported to promote restitution and regeneration of intestinal mucosal epithelium, although the mechanism remains unknown.AIMTo elucidate the protective effects of ITF on gastric mucosa and explore the possible mechanisms.METHODSWe used a rat model of gastric mucosal damage induced by water immersion restraint stress and lipopolysaccharide-treated human gastric epithelial cell line to investigate the potential effects of ITF on damaged gastric mucosa both in vivo and in vitro.RESULTSITF promoted the proliferation and migration and inhibited necrosis of gastric mucosal epithelia in vitro. It also preserved the integrity of gastric mucosa by upregulating expressions of occludin and zonula occludens-1. In the rat model, pretreatment with ITF ameliorated the gastric mucosal epithelial damage and facilitated mucosal repair. The protective effects of ITF were confirmed to be exerted via activation of Akt signaling, and the specific inhibitor of Akt signaling {"type":"entrez-nucleotide","attrs":{"text":"LY249002","term_id":"1257710161","term_text":"LY249002"}}LY249002 reversed the protective effects.CONCLUSIONITF might be a promising candidate for prevention and treatment of stress-induced gastric mucosal damage, and further studies should be undertaken to verify its clinical feasibility.     

胃粘膜p53抑癌基因异常与癌变的研究

目的系统研究各种胃粘膜病变中ｐ５３抑癌基因的变异和意义．方法内镜活检组织１５４例，其中浅表性胃炎３０例，萎缩性胃炎３３例，萎缩性胃炎伴肠上皮化生３１例，萎缩性胃炎伴异型增生３０例，胃腺癌３０例．用免疫组化法检测Ｐ５３蛋白表达；用单链构象多态性分析及ＤＮＡ测序检测ｐ５３第５～８外显子点突变．结果胃良性病变粘膜未见Ｐ５３蛋白表达．胃癌中Ｐ５３蛋白阳性表达率为３３３％（１０／３０例）．单链构象多态性分析ｐ５３点突变的检出率，在胃癌为５４５％（６／１１例），异型增生为２０％（３／１５例），肠化生为６７％（１／１５例），测序证实１例胃癌在第５外显子存在点错义突变和碱基缺失，２例异型增生在第６外显子存在点错义突变．结论ｐ５３抑癌基因变异与胃粘膜癌变有关．     

Transcription factor Sp1 expression in gastric cancer and its relationship to long-term prognosis

AIM: To explore the expression of Spl in gastric carcinoma as well as its association with other clinicopathologic features, and to evaluate the role of Spl as a prognostic indicator of gastric carcinoma.METHODS: By using immunohistochemistry, we examined the Sp1 expression patterns in 65 cases of human gastric cancer, and 40 normal gastric mucosa specimens. Simultaneously, the correlation between Sp1 expression and clinical outcome or clinicopathologic features was investigated.RESULTS: The percentage of Spl expression was 12.5% (5/40) in normal gastric mucosa, and the Sp1 protein was mainly expressed in the nuclei of cells located in the mucous neck region. In sharp contrast, strong Sp1 expression was detected in tumor cells, whereas no or faint Sp1 staining was detected in stromal cells and normal glandular cells surrounding the tumors. The expression rate of Sp1 in gastric cancer lesions was 53.85% (35/65). The medium survival duration in patients who had a tumor with negative, weak and strong Sp1 expressions was 1700, 1560 and 1026d, respectively (P&lt;0.05). Sp1 protein expression was closely related to the depth of tumor infiltration (x^2=13.223, P&lt;0.01) and TNM stage (x^2=11.009, P&lt;0.05), but had no relationship with the number of lymph nodes and Lauren‘s classification (P&gt;0.05). Cox regression model for multivariate analysis revealed that high Spl expression (P&lt;0.05) and advanced stage (P&lt;0.01) were independent predictors of poor survival.CONCLUSION: Normal and malignant gastric tissues have unique Sp1 expression patterns. Spl might serve as an independent prognostic factor, by influencing the tumor infiltration and progression.