冠心病患者细胞粘附分子的水平变化和临床意义

目的：探讨冠心病患者外周血白细胞粘附分子β2整合素(β2-intergrin，CD18)和血清可溶性细胞间粘附分子-1(soluble intercellulal adhesion molecule-1，sICAM-1，CD54)、血管细胞粘附分子-1(soluble vascular cell adhesion molecule-1，sVCAM-1，CD106)变化及其意义。方法：采用流式细胞仪技术检测45例冠心病患者外周血白细胞CD18以及血清sICAM-1和sVCAM-1的表达。结果：冠心病患者外周血白细胞CD18以及血清sICAM-1和sVCAM-1表达显著增加。结论：冠心病患者外周血白细胞CD18以及血清sICAM-1和sVCAM-1的水平对冠心病的早期诊断具有一定的临床意义。     

肺癌血清可溶性细胞间粘附分子-1检测的临床意义

目的观察肺癌肝转移血清可溶性细胞间粘附分子-1(soluble intercellular adhesion molecule-1 sICAM-1)水平,评价检测肺癌肝转移患者(sICAM-1)的临床意义。方法用流式细胞仪(FCMS)对肺癌患者外周血中sICAM-1的水平进行定量测定。结果肺癌组s1CAM-1平均浓度为7.39±6.26,正常对照组s1CAM-1平均浓度为3.37±1.20。肺癌组s1CAM-1水平明显高于对照组,有显著性差异(P<0.01)。肺癌肝转移组s1CAM-1平均浓度为12.10±5.95,无转移癌组s1CAM-1平均浓度为6.36±5.47。肺癌肝转移组s1CAM-1显高于无转移癌组具有显著性差异(P<0.01)。低分化腺癌组略高于高分化腺癌组,二组间差异比较无显著性差异(P>0.05)。腺癌组与鳞癌癌组之间无显著性差异(P>0.05)。结论血清(sICAM-1)水平的检测对评价肺癌患者病情和预后有一定临床意义。     

膀胱癌患者细胞粘附分子水平的变化及其意义

目的：探讨膀胱癌患者外周血白细胞粘附分子β2（整合素（β2－integrin,CD18)和血清可溶性细胞间粘附分子－1（soluble intercellular adhesion molecule-1 sICAM-1,CD54),血管细胞粘附分子（soluble vascular cell adhesion molecule-1,sVCAM-1 CD106)变化及其意义。方法：采用流式细胞技术检测30例膀胱癌患者外周血白细胞CD18以及血清sICAM-1和sVCAM-1的表达，结果：膀胱癌患者外周血白细胞CD18以及血清sICAM-1和sVCAM-1表达显著增加。结论：膀胱癌患者外周血白细胞CD18和血清sICAM-1和sVCAM-1的水平对膀胱癌的早期诊断病理分级、临床分期具有明确的预示作用。     

肝癌患者粘附分子和细胞因子的变化及其意义

目的：观察肝癌患者外周血清可溶性P-选择素(soluble P-selectin，CD62P)，细胞间粘附分子-1(soluble intercellular adhesion molecule-1，sICAM-1，CD54)，血管细胞粘附分子(soluble vascular cell adhesion molecule-1，sVCAM-1，CD106)和白细胞粘附分子β2整合素(β2-integrin，CD18)的变化，并探讨其临床意义。方法：采用流式细胞仪技术检测了48例不同期肝癌患者外周血清sP-selectin，sICAM-1 sVCAM-1水平和白细胞CD18的表达。结果：与正常对照组相比，血清sP-selectin，sICAM-1 sVCAM-1水平和白细胞CD18的表达显著增加。结论：肝癌患者外周血清sP-selectin，sICAM-1 sVCAM11水平和白细胞CD18的表达对肝癌的早期临床分期具有一定的参考价值。     

颅内动脉瘤术后脑血管痉挛与血清可溶性血管细胞粘附分子-1、可溶性细胞间粘附分子-1及丙二醛的关系

目的 探讨颅内动脉瘤(IA)显微外科夹闭术后脑血管痉挛(CVS)的相关因素及与血清可溶性血管细胞粘附分子-1(sVCAM-1)、可溶性细胞间粘附分子-1(sICAM-1)及丙二醛(MDA)水平的关系。方法 2019年2月至2022年10月在我院接受IA显微外科夹闭术的147例患者,依照术后情况分为CVS组(n=45)和非CVS组(n=102),多因素Logistic回归分析术后CVS发生的危险因素;依照脑血管直径将CVS患者分类,并对血清sVCAM-1、sICAM-1以及MDA水平进行检测,分析上述血清指标与CVS疾病严重程度的关系。结果 多因素Logistic回归分析显示Hunt-Hess分级Ⅲ～Ⅳ级、高动脉瘤直径以及血清sVCAM-1、sICAM-1和MDA的高表达均是导致CVS发生的独立危险因素(P<0.05);以上血清指标在严重CVS组中显著高于轻度和中度组(P<0.05);以上血清指标均与CVS疾病严重程度呈正相关(P<0.05)。结论 血清sVCAM-1、sICAM-1及MDA水平是IA显微外科夹闭术后CVS独立危险因素,且与其形成密切相关,可作为CVS严...     

冠心病患者血清可溶性细胞间粘附分子-1和血管细胞粘附分子-1的变化

目的:检测各型冠心病患者循环中可溶性细胞间粘附分子-1(sICAM-1)和血管细胞粘附分子-1(sVCAM-1)水平并分析它们与冠心病活动性之间的关系.方法:用ELISA法测定稳定型心绞痛患者(SAP组),不稳定型心绞痛患者(UAP组),急性心肌梗塞患者(AMI组)和健康体检者(对照组)血清sICAM-1和sVCAM-1浓度.结果:SAP组、UAP组和AMI组血清sICAM-1浓度高于对照组(SAP组与对照组比较P＜0.05;UAP组及AMI组与对照组比较P＜0.001).相关分析表明,sICAM-1水平与冠心病的活动性显著相关(r=0.665,P＜0.001).四组间血清sVCAM-1浓度差异无显著意义.结论:冠心病患者循环中sICAM-1水平升高,并与疾病的活动性相关,而sVCAM-1水平无明显变化.     

卵巢恶性肿瘤患者血清可溶性细胞间粘附分子-1的检测及临床价值

目的检测卵巢恶性肿瘤患者血清可溶性细胞间粘附分子-1(sICAM-1)并分析其临床价值.方法采用双抗体夹心ELISA法检测54例卵巢恶性肿瘤患者sICAM-1,并与对照组及其与临床分期之间的关系进行分析.结果54例卵巢恶性肿瘤患者的sICAM-1含量明显高于对照组(P＜0.01);卵巢恶性肿瘤晚期(Ⅲ～Ⅳ)患者sICAM-1含量也高于早期(Ⅰ～Ⅱ)患者,均有显著性差异(P＜0.05).结论卵巢恶性肿瘤患者血清sICAM-1含量随着肿瘤恶化程度的增加而上升,sICAM-1有可能作为预测卵巢恶性肿瘤患者转归的一个指标.     

甲状腺相关眼病血清sICAM-1、sVCAM-1水平变化及与病情关系分析

目的观察甲状腺相关眼病(TAO)患者血清可溶性细胞间黏附分子1(sICAM-1)及可溶性血管细胞黏附分子1(sVCAM-1)表达情况,并与病情进行相关性分析。方法将该院2016年8月至2018年3月收治的150例TAO患者纳入研究,依据临床活动性评分(CAS)分为活动期组、非活动期组,按病情严重程度分为轻度组、中重度组、极重度组,并选取同期80例健康体检者作为对照组,比较各组一般资料及血清sICAM-1、sVCAM-1水平;分析sICAM-1、sVCAM-1水平与TAO病情严重程度的相关性。结果活动期组血清sICAM-1与sVCAM-1显著高于非活动期组及对照组,对照组与非活动期组比较差异无统计学意义(P0.05)。不同病情严重程度组的活动期患者血清sICAM-1与sVCAM-1显著高于组内活动期患者与对照组(P0.05),极重度组、中重度组、轻度组的水平依次降低,差异有统计学意义(P0.05)。非活动期患者病情程度与血清sICAM-1、sVCAM-1无显著相关性(P0.05);活动期患者病情程度与血清sICAM-1、sVCAM-1呈正相关(P0.05)。结论活动期患者病情越严重,血清sICAM-1、sVCAM-1表达水平越高,而非活动期患者无此特点,可指导TAO临床诊治。     

可溶性血管细胞粘附分子1、白细胞介素6及肿瘤坏死因子α在妊高征发病中的作用

目的：研究可溶性血管细胞粘附分子1(soluble vaseular cell adhesion molecule-1，sVCAM-1)、白细胞介素6(interleukin-6，IL-6)及肿瘤坏死因子α(tumor necrosis factor-α，TNF-α)在妊高征发病中的作用。方法：测定67例孕妇血清中sVCAM-1、IL-6、TNF-α水平，其中正常妊娠组15例，妊高征组52例，包括轻度14例、中度18例、重度20例。用酶联免疫吸附法(EUSA)测定sVCAM-1，用放射免疫法(RIA)测定IL-6及TNF-α。结果：中、重度妊高征患者血中sVCAM-1、IL-6、TNF-α水平均显著高于正常妊娠组(P<0．01)，轻度妊高征患者与正常妊娠组相比，虽无统计学差异，但有升高趋势。妊高征组产后各项指标下降与正常妊娠组差异无显著性(P>0．05)。sVCAM-1与平均动脉压呈正相关(r=0．542，P<0．01)，IL-6、TNF-α与sVCAM-1也呈正相关(r=0．435，P<0．01及r=0．532．P<0．01)。结论：妊高征患者血中sVCAM-1水平升高表明内皮细胞损伤在妊高征的发病中起重要作用。IL-6、TNF-α可诱导sVCAM-1的表达。     

类风湿性关节炎患者血清可溶性细胞间粘附分子-1测定与核素骨显像相关性研究

目的 研究类风湿性关节炎(RA)患者血清可溶性细胞间粘附分子-1(sICAM-1)水平变化及其与99Tcm-MDP骨关节显像的相关性,探讨在本病早期诊断中的意义.方法 用ELISA法检测达标组(23例)、非达标组(28例)及对照组(20例)血清sICAM-1水平.以99Tcm MDP为示踪剂,对上述3组受检者行核素骨关节显像,进行肉眼定性及半定量分析.结果 达标组、非达标组血清sICAM-1水平明显高于对照组(P<0.01和P<0.05),达标组与非达标组比较,血清sICAM-1水平差异无统计学意义(P>0.05).非达标组、达标组血清sICAM-1水平与手足关节指数有相关性(P<0.01或P<0.05).达标组、非达标组手、足、腕、踝病变关节放射性浓聚明显高于对照组.结论 检测血清sICAM-1水平及99Tcm-MDP骨关节显像定性、半定量分析,有助于类风湿性关节炎患者,尤其是早期进行诊断及判断病情活动程度,改善预后.     

Genetic polymorphism of cytochrome P450 1A1 (Cyp1A1) and glutathione transferases (M1, T1 and P1) among Africans.

The co-ordinate expression and regulation of the drug metabolising enzymes, cytochrome P4501A1 (CYPlAl) and glutathione transferases (GSTM1, GSTT1 and GSTP1), and their metabolic balance in the cells of target organs may determine whether exposure to carcinogens results in cancer. Besides showing variability in activity due to induction and inhibition, these enzymes also exhibit genetic polymorphism that alter enzyme levels and activity. We determined frequencies of common allelic variants of CYP1A1 and glutathione (M1, T1 and P1) among Tanzanians, South African Venda and Zimbabweans using PCR/restriction fragment length polymorphism techniques. The CYP1A1 Val462 mutant variant was found at a frequency of 1.3% among 114 subjects. The GSTM1*0 genotype was found at a frequency of 29% and 33% among Tanzanian psychiatric patients and healthy volunteers, respectively. Similarly, the GSTT1*0 polymorphism was present with a frequency of 25% in both the psychiatric patients and healthy controls. The frequency of GSTP1 Val105 variant was 16%, 12% and 21% among Tanzanians, South African Venda and Zimbabweans, respectively. We conclude here that CYP1A1 Val462 polymorphism is very rare among Africans. This is the first report of the GSTP1 Val105 variant frequency in African populations. We show here that there are no differences in frequencies of the variant alleles for CYP1A1, GSTM1, GSTT1 and GSTP1 in the three African populations.     

子宫内膜癌中CYP1A1，CYP1B1及COMT mRNA的表达及临床意义

[目的]探讨细胞色素P450酶（CYP）1A1, 1B1和儿茶酚-O-甲基转移酶（COMT） mRNA的表达与子宫内膜癌发生发展的关系.[方法]采用RT-PCR方法检测48例子宫内膜癌患者和53例健康对照个体的外周血淋巴细胞中CYP1A1,CYP1B1和COMT mRNA的表达.[结果]子宫内膜癌患者外周血淋巴细胞中CYP1A1,CYP1B1 mRNA的表达明显增高,经Logistic回归分析后, CYP1B1 mRNA的高表达能增加子宫内膜癌的发病风险,进一步分析显示Ⅱ期子宫内膜癌CYP1B1 mRNA的表达高于Ⅰ期患者,差异具有显著性意义.[结论]外周血淋巴细胞中CYP1B1 mRNA的高表达与子宫内膜癌的发生发展密切相关.     

周期蛋白D_1,B_1在子宫颈癌中的表达及其与临床病理间的关系

目的探讨CyclinD1,CyclinB1在子宫颈癌中的表达及其与临床病理间的关系。方法收集哈尔滨医科大学附属四院收治的38例子宫颈癌组织。采用S-P免疫组织化学法,选用CyclinD1,CyclinB1多克隆抗体,分别检测其在38例子宫颈癌中的表达率。结果 CyclinD1在38例子宫颈癌中的阳性表达率为76.32%,CyclinB1的阳性表达率为52.63%,CyclinD1的表达率高于CyclinB1,并且其表达差异有统计学意义(经Fisher确切概率法,P<0.05)。CyclinD1,CyclinB1两者的阳性表达率与淋巴结有无转移之间的关系:Cy-clinD1高于CyclinB1,但差异无统计学意义(经Fisher确切概率法,P>0.05)。结论 CyclinD1驱动细胞由G1期进入S期,促进细胞增殖,决定细胞周期进程的速度和方向,在子宫颈癌的发展过程中起着主要的作用。CyclinB1是在有丝分裂过程中起调控作用的周期蛋白,受着cyclinD1作用的影响。     

吸烟和CYP1A1、GSTM1基因多态对肺癌易感性影响

目的:探讨细胞色素P4501A1(cytochrome P-450 1A1,CYP1A1)与谷胱甘肤硫转移酶M1(glutathione S-transferase M1,GSTM1)基因多态与支气管肺癌的关系.方法:采用PCR限制性片段多态检测法,检测肺癌组103例和正常对照组138例患者的CYP1A1与GSTM1基因多态,以非条件性Logistic回归模型分别对年龄、性别进行校正后计算优势比及95%CI.结果:CYP1A1 ml突变型等位基因频率在对照组和肺癌组分别为27.6%和42.7%;GSTM1的缺陷型基因(D)频率在对照组、肺癌组分别为44.2%和61.2%.Logistic回归分析表明,CYP1A1(w1/m1)杂合型(B型)患肺癌的升高3.19倍,纯合突变型(C型)基因患肺癌的升高2.61倍,GSTM1缺陷型(D)患肺癌危险度升高2.09倍,差异均有统计学意义(P<0.05).GSTMl(D)/CYP1A1(B或C)基因型的个体患肺癌危险度为5.72倍.GSTMI(D)和CYP1A1 ml突变型等位基因均可明显增加鳞癌和小细胞癌的患病危险度.结论:CYP1A ml突变型等位基因和GSTM1(D)均是患肺癌的危险因素.CYP1A m1突变型等位基因和GSTM1(D)存在明显的交互作用,二者与吸烟有协同作用.     

Sequences of the NPS-1 and TLE-1 beta-lactamase genes

The NPS-1 and TLE-1 beta-lactamase genes were cloned and sequenced. NPS-1 differed from LCR-1 beta-lactamase in 8 of 260 amino acids. TLE-1 differed from TEM-1 by a single Asp(115)-->Gly substitution and has been renamed TEM-90.     

Vectorial transport of enalapril by Oatp1a1/Mrp2 and OATP1B1 and OATP1B3/MRP2 in rat and human livers

Although Oatp1a1 (rat organic anion-transporting polypeptide 1a1) was the transporter found responsible for the hepatocellular entry of enalapril (EN) into the rat liver, the canalicular transporter involved for excretion of EN and the metabolite, enalaprilat (ENA), was unknown. The Eisai hyperbilirubinemic rat (EHBR) that lacks Mrp2 (multidrug resistance-associated protein 2) was used to appraise the role of Mrp2 in the excretion of [3H]EN and its metabolite [3H]ENA in single-pass rat liver preparations. Although the total and metabolic clearances and hepatic extraction ratios at steady-state were virtually unaltered for EN in EHBR compared with published values of Sprague-Dawley rats, the biliary clearances of EN and ENA were significantly reduced almost to zero (P<0.05). Involvement of human OATP1B1, OATP1B3, and MRP2 in EN transport was further assessed in single- or double-transfected mammalian cells. Human embryonic kidney 293 cells that expressed OATP1B1 or OATP1B3 showed that OATP1B3 transport of EN (20-500 microM) was of low affinity, whereas transport of EN by OATP1B1 was associated with the Km of 262+/-35 microM, a value similar to that for Oatp1a1 (214 microM). The transcellular transport of EN via human OATP1B1 and MRP2, investigated with the double-transfected Madin-Darby canine kidney (MDCK) II cells in the Transwell system, showed that the sinusoidal to canalicular flux of EN in the OATP1B1/MRP2/MDCK cells was significantly higher (P<0.05) than that of mock/MDCK and OATP1B1/MDCK cells. EN was transported by Oatp1a1 and Mrp2 in rats and OATP1B1/OATP1B3 and MRP2 in humans.     

ABCA1, ABCG1, and SR-BI: Transit of HDL-associated sphingosine-1-phosphate

Sphingosine-1-phosphate (S1P) is a zwitterionic lysophospholipid generated by the sphingosine kinase-catalyzed phosphorylation of sphingosine. A number of the biological effects of S1P are mediated by its binding to five specific G protein-coupled receptors located on the cell surface or intracellular targets. However, the synthesis and secretion of S1P require release out of cells for binding with receptors by certain transporters and carriers. High-density lipoprotein (HDL) is an important carrier of S1P in the blood, but the mechanism by which it does so is unclear. This review discusses the mechanism how S1P is transported, and focuses particularly on how the formation of HDL-associated S1P (HDL-S1P) is mediated by certain transporters and carriers. A hypothesis that the ATP-binding cassette transporter A1 (ABCA1), ABCG1, and scavenger receptor class B memberI (SR-BI) play pivotal roles in HDL-S1P formation is also described.