101名健康成人尿海藻糖酶研究分析

目的建立健康成人尿海藻糖酶(trehalase,T)正常参考范围,为临床肾脏疾病的诊治及相关研究提供参考。方法选取健康体检者101名(男58名,女43名,年龄18～56岁),采用Sasai-TakedatsuM测定方法进行尿海藻糖酶检测。结果 101名健康体检者尿T结果为(7.1±4.1)μmol/h.gCr(肌酐),范围0～25μmol/h.gCr,近似正态分布(Z=1.09,P=0.183)男女间差异无统计学意义(P0.05)(t=0.63,P=0.53)。95%的的正常参考范围(X±1.96 S)为0-15μmol/h.gCr。结论健康成人尿海藻糖酶正常参考范围为0-15μmol/h.gCr,供临床参考。     

内蒙古不同浓度砷暴露人群尿砷代谢产物研究

目的 测定内蒙古地区饮用高砷水人群尿砷代谢产物，探讨不同人群砷代谢的特点。方法 采用氢化物发生原子吸收分光光度法检测尿中不同形态的砷代谢产物。结果 2个暴露组人群尿中无机砷（iAs，inorganic arserlic）、甲基砷（MMA，monomethylarsine）、二甲基砷（DMA．dimethylarsine）和总砷（TAs，total arserlic）均高于对照组（P〈0．05）；同样砷暴露水平下，尿中各形态砷含量及其相对比在不同性别问的差异均无统计学意义（P〉0．05），儿童DMA／MMA和DMA％高于成人（P〈0．05），MMA％低于成人（P〈0．05）；2个暴露组儿童、成人分别与对照组比较，暴露组MMA／ias、DMA／MMA、DMA／iAs、DMA％显降低（P〈0．05），而iAs％、MMA％显增高（P〈0．05）；高暴露组与低暴露组相比，儿童DMA／MMA、DMA／iAs、DMA％显增高（P〈0．05），iAs％、MMA％显降低（P〈0．05）。结论 相同砷暴露水平下，男女对砷的甲基化能力无差别，儿童二甲基化能力高于成人。高砷暴露可能降低人群对砷的生物甲基化能力。[编按]     

Ethnicity and alcohol consumption among US adults with diabetes

PurposeThe drinking practices of a nationally representative sample of white, black, Mexican American, and other Hispanic adult diabetics are described and compared.MethodsCombined years (2005/2006–2011/2012) of the National Health and Nutrition Examination Survey provided home interview data from 2220 adults with self-reported diabetes of white (n = 875), black (n = 720), Mexican American (n = 402), and other Hispanic (n = 223) ethnicity. Current drinking status, the number of drinks consumed per week, and binge drinking were compared across ethnicity.ResultsThe multivariate findings for both diabetic men and women showed no statistically significant ethnic differences in current drinking status, and among women, there were no statistically significant ethnic differences in binge drinking. Among male diabetics, Mexican Americans consumed more drinks per week than whites (b = 0.35; 95% confidence interval, 0.13–0.58; P = .002) and were at increased risk for binge drinking (odds ratio, 2.04; 95% confidence interval, 1.30–3.21; P = .002).ConclusionsBinge drinking is prevalent among Mexican American male diabetics. This pattern of drinking may put them at risk for poor diabetes management and control. It is important that health care providers routinely assess their patients' drinking practices and address the health risks associated with alcohol consumption.     

渔村与砷暴露地区居民尿砷代谢产物检测比较

目的 测定渔村居民和饮水型砷暴露地区人群尿中各种形态砷和尿总砷(TAs)。方法 选取饮水型砷暴露地区〔口肯板村水砷浓度为0.16 mg/L;乃莫板村水砷浓度为0.09 mg/L;塔布塞村(对照组)饮用水砷浓度<0.01 mg/L〕和渔村居民。采用氢化物发生原子吸收分光光度法检测尿中各形态砷代谢物和TAs含量。结果 TAs含量渔村居民明显低于0.16 mg/L暴露组,高于对照组(P<0.05),与0.09 mg/L暴露组差异无统计学意义;渔村居民尿无机砷(iAs)含量明显低于0.09和0.16 mg/L暴露组(P<0.05);尿甲基砷(MMA)含量同尿二甲基砷(DMA)含量,各组之间差异均有统计学意义(P<0.05);渔村居民尿iAs百分比明显低于对照组、0.09和0.16 mg/L暴露组(P<0.05);尿MMA百分比同尿iAs百分比;各组尿DMA百分比差异均有统计学意义(P<0.05);渔村居民特异性的检测出尿三甲胺(TMA)。结论 人群尿总砷水平受食用海产品等因素影响,不能单独作为砷暴露指标,应使用分析性方法来区分砷复合物中无机砷和有机砷的代谢产物。     

Exposure to inorganic arsenic in drinking water and total urinary arsenic concentration in a Chilean population

The relationship of inorganic arsenic exposure through drinking water and total urinary arsenic excretion in a nonoccupationally exposed population was evaluated in a cross-sectional study in three mayor cities of Chile (Antofagasta, Santiago, and Temuco). A total of 756 individuals in three population strata (elderly, students, and workers) provided first morning void urine specimens the day after exposure and food surveys were administered. Arsenic intake from drinking water was estimated from analysis of tap water samples, plus 24-h dietary recall and food frequency questionnaires. Multilevel analysis was used to evaluate the effects of the age group and city factors adjusted by predictor variables. Arsenic levels in drinking water and urine were significantly higher in Antofagasta compared with the other cities. City-and individual-level factors, 12% and 88%, respectively, accounted for the variability in urinary arsenic concentration. The main predictors of urinary arsenic concentration were total arsenic consumption through water and age. These findings indicate that arsenic concentration in drinking water continues to be the principal contributing factor to exposure to inorganic arsenic in the Chilean population.     

Risk of erectile dysfunction induced by arsenic exposure through well water consumption in Taiwan

BACKGROUND: Erectile dysfunction (ED) has a profound impact on the quality of life of many men. Many risk factors are associated with ED, such as aging, sex hormone levels, hypertension, cardiovascular diseases, and diabetes mellitus. Arsenic exposure could damage peripheral vessels and increase the risk of cardiovascular disease. However, the relationship between arsenic exposure and ED has seldom been evaluated. OBJECTIVES: In this study we aimed to investigate whether exposure to arsenic enhances the risk of ED. METHODS: We recruited 177 males >/= 50 years of age through health examinations conducted in three hospitals in Taiwan. We used a questionnaire (International Index of Erectile Function-5) to measure the level of erectile function. Sex hormones, including total testosterone and sex hormone-binding globulin, were determined by radioimmunoassay. We used another standardized questionnaire to collect background and behavioral information (e.g., cigarette smoking; alcohol, tea, or coffee drinking; and physical activity). RESULTS: The prevalence of ED was greater in the arsenic-endemic area (83.3%) than in the non-arsenic-endemic area (66.7%). Subjects with arsenic exposure > 50 ppb had a significantly higher risk of developing ED than those with exposure 相似文献   

Food prices and fruit and vegetable consumption among young American adults

Multivariate negative binomial count models were estimated to examine associations between young adults’ fruit and vegetable (FV) consumption and the prices of FV, other food at home grocery items, and fast food and the availability of restaurants and food stores. This study used the 2002 wave of data collected from US young adults aged 18–23 years in the 1997 National Longitudinal Survey of Youth merged by geocode identifiers with food prices and restaurant and food store availability. The results showed that higher levels of FV consumption were associated with lower FV prices (price elasticity of −0.32) and that this own-price effect was robust to the inclusion of other food prices and food outlet availability. Lower income and lower educated young adults and those with lower educated mothers and middle-income parents were found to be most price sensitive. No statistically significant cross-price effects on FV consumption were found with other grocery food (meat, dairy and bread) prices or fast food prices. Fiscal policy instruments such as FV subsidies may help to increase FV intake, particularly among young adults of lower socioeconomic status.     

Concentration of inorganic arsenic in samples of white rice from the United States

Concentrations of inorganic arsenic were determined by ion-trap, electrospray, mass spectrometry in 40 samples of white rice, which were collected in the United States. Inorganic arsenic was qualitatively present in all samples. The quantitative range of concentrations of inorganic arsenic extended from <0.025 to 0.271 μg/g (wet-mass basis). The extremes of the range were validated with acceptable analytical recoveries of fortifications. Statistical bounds of the range were established and assessed. The distribution of concentrations over the observed range was characterized statistically with an empirical lognormal probability distribution function. For the samples of white rice, a geometric mean corresponding to 0.112 μg/g and a geometric standard deviation equivalent to 0.055 μg/g of inorganic arsenic were derived from the empirical lognormal function.     

Relationship between plasma trans-fatty acid isomer concentrations and self-reported cardiovascular disease risk in US adults

Abstract

The effect of different individual TFA isomers on cardiovascular disease (CVD) has been a limited study, especially for stroke. We aimed to investigate the relationships between four major plasma TFA isomer (elaidic, vaccenic, palmitelaidic and linolelaidic acid) concentrations and the risk of CVD, stroke and non-stroke CVD. A cross-sectional study was conducted, utilising a nationally representative sample of US adults in the National Health and Nutrition Examination Survey. Among the 3504 participants, 304 participants self-reported CVD history. The highest quintile of elaidic acid intake was associated with a 233% higher CVD risk (p?=?.010). Adjusted for age, gender and race, palmitelaidic acid was associated with a decreased CVD risk, but the effect size was diminished in a subsequent analysis model. For stroke risk, we failed to identify any associations. In addition to elaidic acid, the health effect of palmitelaidic acid should be paid more attention in the future studies.     

Smoking and alcohol consumption in a UK Chinese population

Little research has been conducted on health in Chinese communities in the UK and there are few representative data on smoking, alcohol consumption or other aspects of lifestyle. We undertook a cross sectional population-based study of 380 Chinese and 625 European men and women aged 25 to 64 y, using self-completion and interview questionnaires in Newcastle upon Tyne, UK between 1991 and 1995. We measured self-reported prevalence of cigarette smoking, number of cigarettes smoked per week and age at starting smoking; self-reported prevalence of alcohol consumption and units of alcohol consumed per week. In age-adjusted comparisons smoking was less common in Chinese (24%) than European men (35%) (P=0.00002) and among Chinese (1%) compared with European women (33%) (P<0.00001). Number of cigarettes smoked was similar among Chinese and European male smokers. Median age at starting smoking was higher among Chinese (18.5 y) compared with European men (15 y) (P=0.00001). Smoking was commonest in older Chinese and in younger Europeans.The prevalence of alcohol consumption was lower among Chinese (63%) than European men (93%) (P<0.00001) and among Chinese (29%) compared to European women (89%) (P<0.00001). Median alcohol consumption was significantly lower among Chinese (2 units/week) than European men (16 units/week) (P<0.00001), and among Chinese (1 unit/week) compared to European women (6 units/week) (P<0.00001). Among those who drank alcohol, Chinese men were less likely to drink above recommended limits than European men (1% vs 39%; P<0.00001). Chinese men and women currently have relatively favourable patterns of smoking compared to European adults in Newcastle. Average alcohol consumption among Chinese who drink is lower than among Europeans, and a substantial proportion of the Chinese population in Newcastle drink no alcohol. Patterns of health related behaviour should be tracked over time in ethnic minority populations to identify changes that pose risk to health and which deserve appropriate intervention.     

Arsenic species excretion in a group of persons in northern Germany--contribution to the evaluation of reference values

Reference values for four arsenic species (inorganic As(III); inorganic As(V); dimethylarsinic acid DMA; monomethylarsonic acid MMA) in urine were evaluated for 101 male persons in northern Germany (46.9 ± 10.5 y) applying anion exchange chromatographic species separation with on-line hydride-technique atomic absorption spectrometry (between-days imprecision 6.8-10.1 %; 11.0-50.0 μg/l; n = 30; detection limits d. l. 1; 10; 2; 2 μg/l). DMA was found in 88.1 % of the persons (x ± s = 7.21 ± 9.64 μg/g creatinine; median 4.02 μg/g; 95 % < 22.5 μg/g) followed by As(III) (11.3 %; median < d. l.; 95 % < 0.54 μg/g) whereas no MMA and As(V) could be found. Seafood consumption within the last 2 days led to higher DMA levels compared to having seafood more than 6 days ago (n = 43 vs. n = 42; 10.04 ± 11.58 vs. 3.47 ± 3.55 μg/g; p < 0.01).     

Low consumption of fruits and dairy foods is associated with metabolic syndrome in Korean adults from outpatient clinics in and near Seoul

BACKGROUND/OBJECTIVES

The aim of this study was to examine differences in nutrient intake and food consumption by the presence of metabolic syndrome in Korean adults.

SUBJECTS/METHODS

Study subjects in this cross-sectional study were recruited from four outpatient clinics in and near the Seoul metropolitan area of South Korea between 2006 and 2012. A total of 668 subjects (413 men and 255 women) aged ≥ 30 y were included in the final data analyses. For each subject, daily nutrient intake and food consumption were calculated using three days of dietary intake data obtained from a combination of 24-hour recalls and dietary records. To evaluate food consumption, mean number of servings consumed per day and percentages of recommended number of servings for six food groups were calculated. Metabolic syndrome was defined using the National Cholesterol Education Program Adult Treatment Panel III criteria. The general linear model was performed to examine differences in nutrient intake and food consumption by sex and the presence of metabolic syndrome after adjustment for potential confounding variables.

RESULTS

Nutrient intake did not differ by the presence of metabolic syndrome in both men and women. Men with metabolic syndrome had lower consumption and percentage of the recommendation for fruits compared with those without metabolic syndrome (1.6 vs. 1.1 servings/day, P-value = 0.001; 63.5 vs. 49.5%, P-value = 0.013). Women with metabolic syndrome showed lower consumption and percentage of the recommendation for dairy foods than those without metabolic syndrome (0.8 vs. 0.5 servings/day, P-value = 0.001; 78.6 vs. 48.9%, P-value = 0.001).

CONCLUSIONS

Low intakes of fruits and dairy foods might be associated with the risk of having metabolic syndrome among Korean adults. Dietary advice on increasing consumption of these foods is needed to prevent and attenuate the risk of metabolic syndrome.     

Applying the theory of planned behavior to predict dairy product consumption by older adults

Objective: The purpose of this study was to explain intention to consume dairy products and consumption of dairy products by older adults using the Theory of Planned Behavior (TPB). The factors examined were attitudes, subjective norms, and perceived behavioral control.

Design: A cross-sectional questionnaire was administered.

Setting: Community centers with congregate dining programs, group classes, and recreational events for older adults.

Subjects: One hundred and sixty-two older adults (mean age 75 years) completed the questionnaire. Subjects were mostly women (76%) and white (65%), with about half having less than a high school education or completing high school.

Variables Measured: Variables based on the TPB were assessed through questionnaire items that were constructed to form scales measuring attitudes, subjective norms, perceived behavioral control, and intention to consume dairy products. Dairy product consumption was measured using a food frequency questionnaire.

Analysis: Regression analyses were used to determine the association between the scales for the 3 variables proposed in the TPB and intention to consume and consumption of dairy products; the level was set at .05 to determine the statistical significance of results.

Results: Attitudes toward eating dairy products and perceived behavioral control contributed to the model for predicting intention, whereas subjective norms did not. Attitudes toward eating dairy products were slightly more important than perceived behavioral control in predicting intention. In turn, intention was strongly related to dairy product consumption, and perceived behavioral control was independently associated with dairy product consumption.

Conclusions and Implications: These results suggest the utility of the TPB in explaining dairy product consumption for older adults. Nutrition education should focus on improving attitudes and removing barriers to consumption of dairy products for older adults.     

阜康市某炼铜企业对周围居民尿砷含量的影响

目的了解阜康市某炼铜企业生产中产生的砷对周围居民尿砷含量的影响。方法用尿中砷的新银盐分光光度法测定阜康市某炼铜企业周围居民尿砷含量。结果居民110人尿砷均值为（36．1±26．5）μg/L,95％CI为31．1-41．1μg/L,中位数为36．0μg/L。结论建议进一步开展大范围一般人群尿砷值调查。     

Intakes of total and individual flavonoids by US adults

Knowing the dietary flavonoid intake of individuals and populations is the first step to clearly understand their health effects. Dietary flavonoid intake studies have been hampered by the lack of flavonoid food composition data. The objectives of this study were to estimate intakes of individual, classes and total flavonoids in the US adults; to evaluate the effects of socio-demographic factors on the flavonoid consumption patterns; and to establish major dietary sources of flavonoids. We calculated flavonoid intakes using the most updated USDA flavonoid and isoflavone databases, and the National Health and Nutrition Examination Survey (NHANES) III 24-h dietary recall (DR). Estimated mean total flavonoid intake by US adults was 344.83?±?9.13?mg/day. The flavan-3-ols (191.99?±?6.84?mg/day) were the most abundant flavonoid class. The three most dominant individual flavonoids were catechin, epicatechin and polymers. Tea, wine, beer, citrus fruits and apples were the most important sources of total flavonoid intakes in the US adults.     

大米中无机砷测定方法的研究

目的通过比较不同浓度三氟乙酸(TFA)溶液和在不同提取时间下TFA对于标准参考物质(SRM1568a rice flour)中无机砷化合物的提取能力,优化TFA溶液的浓度和提取时间,提出大米中无机砷测定方法。同时采集不同原产地的大米样品,评价无机砷污染状况。方法加入TFA,热浸提后取上清液离心浓缩至干,以流动相超声复溶,过RP柱和滤膜后进样;1mmol/L磷酸二氢铵溶液(pH9.0)和20mmol/L磷酸二氢铵溶液(pH8.0)为流动相梯度洗脱,采用Hamilton PRPX-100阴离子交换柱分离,液相色谱-原子荧光光谱法(LC-AFS)测定。结果采用3%(V/V)TFA溶液对SRM1568a热浸提2h的样品前处理方法,其测定结果符合无机砷参考值。结论方法适用于大米中无机砷的测定。所采集大米样品的无机砷含量均未超出卫生标准。     

A study on the trend analysis regarding the rice consumption of Korean adults using Korean National Health and Nutrition Examination Survey data from 1998, 2001 and 2005

The objective of this study was to provide information regarding trends of rice consumption of Korean adults based on different meal types. Respondent reports 24-hour recall data from the Korean National Health and Nutrition Examination Survey were used to assess daily rice consumption and intake ratios of rice for different meal types and places of preparation. Rice intake had gradually decreased from 224.6 g in 1998 to 190 g in 2001 and to 179.4 g in 2005. The rice consumption of Korean adults decreased every year in all ages for all places of meal preparation in 2001 and 2005 compare to 1998. Analysis for each meal type showed that rice intake at breakfast had not considerably changed, but rice intake had decreased at lunch and dinner. While the rice consumption ratio at lunch and dinner decreased, it also decreased or did not change at snack times except for the 19-29 age groups. All the age groups revealed comparable change in the analysis for meal types. There was some diversity between all age divisions in daily rice intake depending on place of meal preparation. The rice consumption by place of meal preparation was generally highest at home, lowest at other places, but it decreased in all places. The rice consumption at home was highest in the over 50 age group, lowest in the 20-30 age group. These changes seem to be related to food intake patterns of rice and substitutional foods in the diets and development regarding socio-economic status. So the need for further study on differences in rice intake based on socioeconomic levels and age group are indicated.     

Dietary Polyphenol Intake in US Adults and 10-Year Trends: 2007-2016

BackgroundPolyphenols are a class of phytochemicals that have antioxidant, anti-inflammatory, anticancer, and antiviral properties. Previous research suggests that dietary polyphenol intake is protective against major chronic diseases. To our knowledge, no data on polyphenol intake for the US adult population are available.ObjectiveThis study explored usual dietary polyphenol intake among US adults in 2013-2016 and examined trends in intake during 2007-2016 by demographic characteristics, and identified major dietary sources of polyphenols.DesignThe National Health and Nutrition Examination Survey is a series of cross-sectional surveys representative of the civilian noninstitutionalized US population.Participants/settingThis study included 9,773 adults aged 20 years and older.Main outcome measuresDietary and supplement data were obtained from two 24-hour dietary recalls. Polyphenol intake was estimated using the Phenol Explorer Database and adjusted for total energy intake.Statistical analysis performedUsual intake was estimated both overall and by demographic characteristics using the National Cancer Institute method. Trends in intake on a given day over 10 years were evaluated using regression analysis. The complex survey design was incorporated in all analyses.ResultsIn 2013-2016, the usual intake of dietary polyphenols was a mean (standard error) of 884.1 (20.4) mg per 1,000 kcal/d. Polyphenol intake was higher in adults 40 years and older, women, non-Hispanic White adults, and college graduates. During 2007-2016, the mean daily polyphenol intake did not change significantly over time for overall and demographic groups. Main polyphenol classes consumed were phenolic acids (mean [standard error] of 1,005.6 [34.3] mg/d) and flavonoids (mean [standard error] of 379.1 [10.7] mg/d). Foods and beverages contributed 99.8% of polyphenol intake, with coffee (39.6%), beans (9.8%), and tea (7.6%) as major dietary contributors.ConclusionFindings from this study suggest that polyphenol intake is consistent with the low intake of fruits, vegetables, and whole grains in the US population, and provide more evidence of the need for increased consumption of these food groups.     

Comparison of several methods for the determination of arsenic compounds in water and in urine

Summary Several arsenic species (inorganic tri- or pentavalent arsenic, mono-and dimethylated arsonic acids) can be determined in water samples by electrothermal atomic absorption spectrometry after appropriate acidification procedures (concentrated HCl or a mixture HCl/HClO₄/HBr) and extraction by toluene in the presence or absence of KI; the determination of aromatic derivatives and of arsenic thiol complexes needs a wet or dry ashing step. The procedures for water analysis are not directly applicable to urine samples; in the best conditions, total inorganic plus 85% on the average of the methylated arsenicals present in urine are measured after acidification with concentrated HCl and extraction by toluene in the presence of KI. Total arsenic content (including arsenic from marine origin) is measured only after a drastic mineralization step like MgO treatment at 600°C. The results obtained by the electrothermal atomic absorption technique and those obtained by neutron activation analysis are in excellent agreement.When the presence of arsenic of marine origin is suspected in urine, the analysis of inorganic arsenic and its metabolites is preferably performed by an arsine generation technique. The sum of inorganic arsenic and of its mono and dimethylated derivatives determined by such a technique is identical with the results obtained by electrothermal atomic absorption spectrometry after complete mineralization of the samples as long as no arsenic from marine origin is present.After oral ingestion of As₂O₃ by man, the urinary excretion of inorganic arsenic and its metabolites is important and rapid (approximately 60% are eliminated by the oral route with a half life of 30 h).While the excretion occurs in the form of inorganic species during the first hours following the ingestion, a methylating process is rapidly triggered and leads to a preponderant excretion of dimethylarsinic acid 1 day after ingestion. In the case of ingestion of seafood containing arsenic, the urinary excretion occurs at a higher rate (half life 18 h) apparently without transformation.The absence of interference of arsenic from marine origin and the capacity of measuring separately inorganic arsenic and its main urinary metabolites makes the arsine generation technique the best suited for the monitoring of workers exposed to inorganic arsenic. However, since the technique may sometimes be too elaborate and time-consuming for routine work, the biological monitoring of workers can be performed by determining total arsenic concentration in urine after mineralization with MgO. Samples with high arsenic content are then re-analyzed to distinguish between occupational exposure and ingestion of the organic arsenic present in marine organisms. This is carried out either by the arsine generation method or, if this technique is not available, by a direct extraction procedure in the presence of KI of a sample acidified with HCI. With the latter procedure, 85% of the methylated arsenic is measured on average without interference of arsenic from marine origin.