Effect of combined injection of hydroxyapatite and estrone on dental well healing

Combined injection of hydroxyapatite and estrone stimulates bone tissue regeneration in albino rats with tunnel defects of bone tissue after removal of mandibular incisors.³H-thymidine autoradiography demonstrates that bone tissue regenerates due to proliferation and differentiation of osteogenic bone marrow precursor cells and connective tissue pericytes. Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 123, No. 6, pp. 693–697, June, 1997     

Role of mast cells in reparative processes in inflammation

Using the model of acute infectious peritonitis in rats, it is shown that inflammation induced in the absence of mast cells is characterized by marked inhibition of reparative processes. The most significant accumulation of functionally active fibroblasts and the development of granulations and young connective tissue in the mesentery occur 5–10 days after flogogen injection in the natural development of inflammation and after 10–20 days in the absence of mast cells. The data suggest that under natural conditions mast cells directly or indirectly stimulate reparative processes. Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 119, N ^o 3, pp. 262–265, March, 1995 Presented by E. D. Gol'dberg, Member of the Russian Academy of Medical Sciences     

Correction of testosterone-induced changes in a population of hemopoietic precursors by a bone-marrow inhibitor of the proliferative activity of a hemopoietic stem cell

A factor inhibiting the proliferation of splenic colony-forming units, injected bothin vivo and after preincubation of mouse bone marrow cellsin vitro, had a dose-dependent effect on the increased proliferative activity of splenic colony-forming units from the bone marrow of mice treated with testosterone propionate. This was associated with a reduction in the number of early hemopoietic precursors of mouse bone marrow. The counts of clonogenic granulocytic-macrophagal and macrophagal colony-forming units decreased and that of burst-forming units in murine bone marrow increased after exposure to the hormone. Testosterone propionate promoted a decrease of the repopulating potential of bone marrow cells, which recovered after their preincubation with the factor inhibiting the proliferation of splenic colony-forming units. Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 120, N ^o 10, pp. 394–397, October, 1995     

Effect of low-intensity luminescence on reparative processes in skin wounds in the rat

Low-intensity noncoherent luminescent radiation stimulates reparative processes in soft tissue wounds of rats. The stimulation is dependent on the frequency of light pulsation and the luminescence spectrum. Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 117, N ^o 6, pp. 665–667, June, 1994 Presented by B. A. Korolev, Member of the Russian Academy of Medical Sciences     

Decrease of antitumor and suppressor activities of nonadherent bone marrow cells by culturing in medium conditioned with ehrlich adenocarcinoma cells

It was demonstrated that proliferation of bone marrow cells cultured in medium conditioned with tumor cells is somewhat increased. A much more potent stimulation of bone marrow cell proliferation is observed after the removal of cells carrying the erythroblast antigen. Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. N ^o 5, pp. 514–517, May, 1994 Presented by N. V. Vasil'ev, Member of the Russian Academy of Medical Sciences     

Immuno- and phagocytosis-modulating properties of low doses of B1-aflatoxin and benzene

Benzene or benzene-dissolved B₁-aflatoxin in low doses promotes an increase of the Thy-1⁺ cell count in the bone marrow of mice and an enhancement of the thymusdependent immune response.In vitro aflatoxin and benzene are unable to induce the expression of Thy-1-antigen in bone marrow T-precursors. Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 117, N ^o 2, pp. 158–160, February, 1994 Presented by S. A. Neifakh, Member of the Russian Academy of Medical Sciences     

Comparative study of the state of bone tissue during hormone replacement therapy and transplantation of human fetal tissues in oophorectomized women

The state of bone tissue is studied in 95 women with postoophorectomy syndrome. It is shown that fetal tissue therapy delayed the progression of the pathological process in bones and even increased bone density. Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 120, N ^o 10, pp. 428–430, October, 1995     

Antimutagenic activity of β-carotene

Data are presented on the ability of synthetic β-carotene to reduce the level of cyclophosphane-induced chromosome aberrations in murine bone marrow cells. InSalmonella typhimurium cells β-carotene exhibits antimutagenic activity only against an indirect mutagen (2-acetylaminofluorene). Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 120, N ^o 9, pp. 276–278, September, 1995 Presented by Yu. N. Solov'ev, Member of the Russian Academy of Medical Sciences     

Antitumor activity of normal bone marrow cells

After separation of normal murine bone marrow cells in a Percoll density gradient cellular fractions with densities of 1.076 and 1.060 g/ml are capable of suppressing thein vitro growth of leukemia cells. The cytostatic activity of these fractions, however, does not surpass the level of antitumor antiproliferative activity intrinsic to intact bone marrow cells. These cells were found to be capable of joining the splenocytes, thymocytes, and lymph node cells in effector cytostatic cooperation and thus enhance the final antitumor effect. Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 120, N ^o 8, pp. 181–183, August, 1995     

Radiometric evaluation of the effect of xenobiotics on proliferation of bone marrow cells in mice

A radiometric method of evaluating the effect of chemical compounds on the proliferative activity of bone marrow cells is described which consists of measuring the incorporation of labeled³H-thymidine in DNA. Results are reported on a comparative study of the effect of known immunotropic substances on bone marrow cell proliferation using the present method and the method of evaluating endogenous colony formation. Analysis of the results obtained by two variants ofin vivo andin vitro experiments provides additional information regarding the mechanism of action of the substances. Translated fromByulleten Eksperimental'noi Biologii i Meditsiny, Vol. 120, N ^o 8, pp. 222–224, August, 1995 Presented by Yu. A. Romanov, Member of the Russian Academy of Medical Sciences     

Evaluation of graft-versus-host disease based on measurement of HLA levels in the plasma of allogeneic bone marrow recipients

HLA levels were estimated in the plasma of allogeneic bone marrow recipients with and without graft-versus-host disease (GVHD). It was found that the level of plasma-soluble HLA is elevated in recipients with developing acute GVHD and that a rise in these antigens coincides with the onset of clinically manifest GVHD. Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 120, N ^o 12, pp. 596–598, December, 1995     

Formation of colonies of clonogenic stromal precursors in bone marrow and splenic cell cultures in the presence of streptococcal antigens in the culture medium

The presence of streptococcal M protein and A polysaccharide in culture medium is shown to have an inhibitory effect on the growth of clonogenic stromal precursors in cultures of healthy murine bone marrow and of healthy guinea pig bone marrow and spleen. The efficacy of colony formation dropped 1.5- to 2-fold in the presence of antigens in a concentration of 25 μg/ml in the medium. The inhibitory effect was absent if antigens were added to adhesive cell cultures. The addition of antigens to cultures originating from animals immunized with streptococcus resulted in inhibition of the efficacy of colony formation in complete cultures and in cultures of adhesive cells. The presence of streptococcal antigens in guinea pig stromal fibroblast cultures of different strains did not affect their growth or colony formation. These data indicate that the effects of streptococcal antigens appear to be aimed at the stromal cells not directly, but rather via another cellular category in the bone marrow and splenic cell cultures, probably lymphocytes. Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 118, N ^o 11, pp. 489–492, November, 1994     

Morphological characteristics of histions of developing and atresic human ovarian follicles

The morphology of tissue elements of human ovarian follicles was studied in the course of normal folliculogenesis and in some forms of its disorders. Signs of abnormal development of follicles were detected that are typical of different stages of their development under the conditions studied. Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 120, N ^o 8, pp. 188–191, August, 1995     

Effect of purine antagonists on the cAMP level in lymphocytes and bone marrow lymphoblasts

The effect of the purine receptor ligands N-ethylcarboxamide adenine and adenosine and of the purine antagonists mercaptopurine and azathioprine on the intracellular cAMP content in human peripheral blood lymphocytes and bone marrow lymphoblasts was studied. All preparations tested induced an increase in the cAMP level in peripheral blood lymphocytes. The selective immunosuppressive effect of adenosine antagonists may be due to their ability to modulate the activity of adenylate cyclase in lymphoid cells. Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 119, N ^o . 3, pp. 294–296, March, 1995     

Size of lymphocyte subpopulations in the spleen and level of hemopoietic tissue proliferation in mice during surgical interventions

The percent and absolute content of T helpers increases in the spleen of mice 4 and 17 h after resection of the liver or removal of a kidney, which is associated with an increase of the mitotic index of splenocytes. The number of T suppressors and the bone marrow cell mitotic index are unchanged. The T-helper/T-suppressor ratio in the spleen increases 1.7–2 times during surgery on the liver and kidney. Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 121, N ^o 3, pp. 301–303, March, 1996 Presented by N. K. Permyakov, Member of the Russian Academy of Medical Sciences     

Antimutagenic properties of ubiquinone-10

The effect of biotechnologically obtained ubiquinone-10 on the level of photrin- and dioxydin-induced chromosomal aberrations is studied for different doses of the preparations by assessing chromosomal aberrations in bone marrow cells of C57B1/6 mice. It is shown that ubiquinone-10 does not exhibit its own mutagenic effect and does not potentiate the effect of the test mutagens. Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 118, N ^o 9, pp. 270–273, September, 1994     

On the erythron response to inflammation and its mechanisms

Using the mouse model of acute infectious peritonitis caused byEscherichia coli, it is shown that the development of inflammation is accompanied by increases in the number of erythrokaryocytes, erythroid colony-forming units, and erythroid hematopoietic islets in the bone marrow and by rises in the activities of supernatants of cultured stimulated adherent and nonadherent myelokaryocytes and of peripheral blood. The results of this study indicate that a characteristic, feature of acute inflammation is strong activation of erythropoiesis with the development of hyperplasia of the erythroid marrow. Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 120, N ^o 10, pp. 382–384, October, 1995     

Stem hematopoietic cells with inserted foreign gene: Proliferative activity and proliferative potential in the long term after transplantation into irradiated mice

The transfer of the human adenosine deaminase gene to murine stem hematopoietic cells is performed on an irradiated sublayer of a long-term bone marrow culture by the conventional method of retroviral transduction with cytokines and by stimulation of stem cells without cytokines. The efficiency of gene transfer into colony-forming units (CFUs) with the aid of cytokines is 72% and without them it is 50%. In irradiated mice reconstituted with the retrovirus-infected bone marrow cells the donor hematopoietic activity is preserved during a 1-year period. The proliferative activity of CFUs of chimeric cells 6 months after the reconstitution was the same and did not depend on the mode of gene transfer. The spleen repopulation activity is lowered in all the groups of chimeric mice 6–12 months after reconstitution. Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 117, N ^o 6, pp. 648–650, June, 1994 Presented by A. I. Vorob'ev, Member of the Russian Academy of Medical Sciences     

Modern concepts of the structural bases for the reparative regeneration of the mammalian and human testis

Modern concepts of the structural bases of the reparative regeneration of the mammalian and human testis are discussed in this article, based on published data and our own findings. The significance of the rete testis as a regeneration zone is discussed as well as the use of its potentials in clinical andrology. Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 117, N ^o 4, pp. 396–399, April, 1994     

Functional activity of blood mononuclear cells during regeneration of bone tissue

It is established that, during elongation of a segment of the extremity, normal regeneration of the bone tissue is attended by a slight decrease in monocyte adherence during the postoperative period and by an increase of the functional activity of lymphocytes during subsequent activation of osteogenesis. Suppression of the lymphoid apparatus is observed in the case of disturbed osteogenesis. Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 1117, N ^o 1, pp. 104–105, January, 1994