| 光气中毒致肺泡上皮细胞线粒体结构和功能损伤的研究 |
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| 引用本文： | 龙子,孔德钦,海春旭,王欣. 光气中毒致肺泡上皮细胞线粒体结构和功能损伤的研究[J]. 癌变．畸变．突变, 2015, 27(1): 54-58. DOI: 10.3969/j.issn.1004-616x.2015.01.012 |
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| 作者姓名： | 龙子 孔德钦 海春旭 王欣 |
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| 作者单位： | 第四军医大学学员一旅四营十三连，陕西 西安 710032 |
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| 摘 要： | 目的： 研究光气中毒对肺泡上皮细胞线粒体结构和功能的损伤作用。方法：30只SD大鼠随机分为正常对照组和染毒组，每组各15只。正常对照组以空气为对照。染毒组给予光气(10 g/m3 )动态染毒5 min，构建光气致急性肺损伤模型。染毒后2 h麻醉，采集肺组织样本，测定肺组织湿/干比，进行肺组织病理检测和电镜观察，并测定肺组织线粒体酶复合物活性。结果：与对照组比较，光气染毒后，肺湿/干比显著增加(P<0.01)，肺组织存在炎细胞浸润和肺泡上皮细胞损伤；线粒体明显肿胀，形态改变，甚至固缩溶解，数量减少，线粒体嵴断裂和缺失，线粒体酶复合体活性降低(P<0.05)。结论：肺泡上皮细胞线粒体损伤为光气中毒的主要毒作用靶点之一，本研究结果为光气中毒防治提供了新思路。
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| 关 键 词： | 光气 急性肺损伤 线粒体 中毒 |
| 收稿时间： | 2014-10-03 |
| 修稿时间： | 2014-12-25 |
Study on phosgene-induced damage of pulmonary mitochondria |
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| LONG Zi,KONG Deqin,HAI Chunxu,WANG Xin. Study on phosgene-induced damage of pulmonary mitochondria[J]. Carcinogenesis,Teratogenesis and Mutagenesis, 2015, 27(1): 54-58. DOI: 10.3969/j.issn.1004-616x.2015.01.012 |
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| Authors: | LONG Zi KONG Deqin HAI Chunxu WANG Xin |
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| Affiliation: | The Thirteenth Company, the Fourth Battalion, First Brigade, Fourth Military Medical University, Xi’an 710032 |
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| Abstract: | OBJECTIVE:To study the toxicological effects of phosgene on pulmonary mitochondria. METHODS:30 SD rats were randomly divided into two groups,with 15 in each group. The rats in control group were 3exposed to air. The rats in phosgene group were exposed to 10 g/m phosgene for 5 min.Two hours after exposure,pulmonary tissues were collected,HE stainingand transmission electron microscope were used to identify histological changes. Activities of mitochondrial complexes were determined. RESULTS:Phosgene induced a significant increase of pulmonary wet/dry ratio. After phosgene poisoning,there were profuse neutrophil infiltration and pulmonary alveoli epithelial cell damage. Mitochondriawerenotably swollen,and even appeared contracted and dissolved. The number of mitochondriawassignificantly decreased. Mitochondrial ridge was broken and absent. The activities of mitochondrial complex were significantly reduced.CONCLUSION:Mitochondriawere main targets of phosgene poisoning,and the results provided basis for the development of new drugs in the treatment of phosgene poisoning. |
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| Keywords: | phosgene acute lung damage mitochondria poisoning |
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