|
|||||
|
|
| HPLC测定盐酸齐拉西酮血药浓度 | |
| 引用本文： | 张国阳, 陈桂兵, 李坚, 王利. HPLC测定盐酸齐拉西酮血药浓度[J]. 中国现代应用药学, 2010, 27(10): 939-941. |
| 作者姓名： | 张国阳 陈桂兵 李坚 王利 |
| 作者单位： | 1.江苏省淮安市第三人民医院，江苏 淮安 223001 |
| 摘 要： | 目的 建立盐酸齐拉西酮血药浓度的测定方法。方法 采用反相高效液相色谱法测定盐酸齐拉西酮血药浓度，色谱柱采用Kromasil C18(4.6 mm×250 mm，5 μm)，流动相：甲醇-乙腈-40 mmol·L-1乙酸铵溶液(用乙酸调节pH值至3.50±0.1) (100∶100∶155)；流速：1.0 mL·min-1；内标：卡马西平；检测波长：254 nm。结果 以盐酸齐拉西酮与内标物峰面积之比(Y)对盐酸齐拉西酮血清浓度(C)进行线性回归，得回归方程：C=213Y-85.5(r=0.996 2)，表明本法在20.0~1 200 μg·L-1内线性关系良好，平均加样回收率为97.4%。结论 本法操作简便、结果准确，可以用于临床盐酸齐拉西酮血药浓度的测定。 |
| 关 键 词： | 高效液相色谱法 盐酸齐拉西酮 血药浓度 |
Determination of Ziprasidne Hydrochloride Concentrations in Plasma by HPLC |
|
| ZHANG Guoyang, CHEN Guibing, LI Jian, WANG Li. Determination of Ziprasidne Hydrochloride Concentrations in Plasma by HPLC[J]. Chinese Journal of Modern Applied Pharmacy, 2010, 27(10): 939-941. | |
| Authors: | ZHANG Guoyang CHEN Guibing LI Jian WANG Li |
| Affiliation: | 1.The Third People’ s Hospital of Huaian, Huaian 223001, China |
| Abstract: | OBJECTIVE To develop an HPLC method for the quantitative determination of ziprasidne hydrochloride in plasma.METHODS The residues were analyzed with a RP-HPLC used Kromasil C18 column（4.6 mm×250 mm,5 μm） with the mobile phase consisted of methanol-acetonitrile-40 mmol.L-1 ammonium acetate（With acetic acid adjust to pH 3.5±0.1）（100 ∶ 100 ∶ 155）.The flow rate was 1.0 mL·min^-1,and carbamazepine was used as internal standard.The detection wavelength was set at 254 nm.RESULTS Linear regression analysis was made by ziprasidne hydrochloride plasma concentration（C） and the peak area ratio（Y） of ziprasidne hydrochloride to internal standard（carbamazepine）,the regression equation was C=213Y-85.5（r=0.996 2）.The results showed that ziprasidne has a good linear relationship in the range of 20.0-1200 μg.L-1.The average recovery of ziprasidne hydrochloride was 97.38%.CONCLUSION The method provides a simple and accurate way for clinical monitoring of ziprasidne hydrochloride in plasma. |
| Keywords: | HPLC ziprasidne hydrochloride plasma concentration |
| 本文献已被 维普 等数据库收录！ | |
| 点击此处可从《中国现代应用药学》浏览原始摘要信息 | |
| 点击此处可从《中国现代应用药学》下载全文 | |