| BALB/c小鼠对马尔尼菲篮状菌黑素的免疫应答 |
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| 引用本文： | 孙弦,刘睿,吴庭,刘栋华. BALB/c小鼠对马尔尼菲篮状菌黑素的免疫应答[J]. 中国麻风皮肤病杂志, 2018, 34(11): 660-662 |
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| 作者姓名： | 孙弦 刘睿 吴庭 刘栋华 |
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| 作者单位： | 1.武汉大学附属同仁医院（武汉市第三医院）皮肤科，武汉 430000;2.广西医科大学一附院皮肤科，南宁 530021 |
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| 摘 要： | 目的:明确马尔尼菲篮状菌酵母细胞黑素能否刺激BALB/c小鼠产生免疫应答。方法:利用马尔尼菲篮状菌酵母细胞黑素颗粒作为抗原免疫BALB/c小鼠，制备马尔尼菲篮状菌黑素抗体，采用凝集试验和ELISA间接法进行检测。结果:免疫接种后的小鼠出现不同程度腹水、脱毛或脾脏肿大等；免疫小鼠的腹水、血清和杂交瘤细胞培养上清与马尔尼菲篮状菌黑素颗粒均产生凝集反应，ELISA法检测抗体OD值依次为1.635±0.085、1.820±0.249、0.671±0.142。结论:马尔尼菲篮状菌黑素可以刺激BALB/c小鼠产生抗体。
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| 关 键 词： | 马尔尼菲青霉 黑素 抗体 ELISA试验 |
| 收稿时间： | 2018-08-03 |
Immune response to Talaromyces marneffei melanin in BALB/c mice |
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| SUN Xian,LIU Rui,WU Ting,LIU Donghua. Immune response to Talaromyces marneffei melanin in BALB/c mice[J]. China Journal of Leprosy and Skin Diseases, 2018, 34(11): 660-662 |
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| Authors: | SUN Xian LIU Rui WU Ting LIU Donghua |
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| Affiliation: | 1.Department of Dermatology,Tongren Hospital of WuHan University (Wuhan Third Hospital ), Wuhan 430000 China; 2. Department of Dermatology, First Affiliated Hospital of Guangxi Medical Univer sity, Naning 530021 China |
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| Abstract: | Objective: To investigate the immune response to Talaromyces marneffei?melanin in BALB/c mice. Methods: The BALB/c mice were stimulated by Talaromyces marneffei cells melanin granules. The Talaromyces marneffei melanin antibodies were collected and detected by agglutination test and indirect ELISA. Results: The mice were immunized with different degrees of ascites, hair removal or spleen enlargement. The ascites, mice serum and supernatant of myeloma fusion cells had agglutination reaction with Talaromyces marneffei melanin granules And the OD of the antibody were 1.635±0.085、1.820±0.249、0.671±0.142 respectively. Conclusion: The Talaromyces marneffei melanin may stimulate immune system to produce antibodies. |
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| Keywords: | Talaromyces marneffei melanin antibody ELISA |
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