| 生存素基因在三氧化二砷诱导腺样囊性癌-2细胞凋亡中的作用 |
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| 引用本文： | 张斌,牟海滨,徐旭光,刘伟,胡那日苏. 生存素基因在三氧化二砷诱导腺样囊性癌-2细胞凋亡中的作用[J]. 华西口腔医学杂志, 2010, 28(3): 246-249. DOI: 10.3969/j.issn.1000-1182.2010.03.005 |
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| 作者姓名： | 张斌 牟海滨 徐旭光 刘伟 胡那日苏 |
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| 作者单位： | 哈尔滨工业大学,市政环境工程学院,黑龙江,哈尔滨,150090;哈尔滨医科大学第二临床医学院,口腔颌面外科;哈尔滨医科大学第二临床医学院,口腔颌面外科,黑龙江,哈尔滨,150086;哈尔滨医科大学第二临床医学院,临床诊断试验中心,黑龙江,哈尔滨,150086;哈尔滨医科大学第二临床医学院,口腔种植科,黑龙江,哈尔滨,150086 |
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| 摘 要： | 目的体外观察三氧化二砷（As2O3）诱导人类腺样囊性癌（ACC）-2细胞凋亡的作用,同时检测此过程中ACC-2内生存素（Survivin）表达水平的变化。方法甲基噻唑基四唑（MTT）法检测As2O3不同处理条件下对ACC-2细胞生长的抑制效应,流式细胞术观察As2O3诱导的各组ACC-2细胞的凋亡率。使用逆转录聚合酶链反应（RT-PCR）检测Survivin mRNA的表达,蛋白印迹（Western blot）检测蛋白水平的表达差异。结果As2O3作用下ACC-2细胞生长明显受抑制,其抑制率呈浓度和时间依赖关系,细胞凋亡率也呈相同的趋势。RT-PCR和Western blot检测显示,As2O3作用下ACC-2细胞内Survivin mRNA和蛋白表达明显受抑制,抑制程度也具有时间和剂量依赖性。结论As2O3可通过促进细胞凋亡使体外培养的ACC-2细胞生长受抑制,受抑制的ACC-2细胞内Survivin mRNA和蛋白表达下降。推测Survivin基因在As2O3诱导的ACC-2细胞凋亡中起重要作用。
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| 关 键 词： | 三氧化二砷 腺样囊性癌 细胞凋亡 |
| 收稿时间： | 2010-06-25 |
| 修稿时间： | 2010-06-25 |
Role of Survivin gene on the apoptosis of adenoid cystic carcinoma-2 cells induced by arsenic trioxide |
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| ZHANG Bin,MU Hai-bin,XU Xu-guang,LIU Wei,HU Na-ri-su. Role of Survivin gene on the apoptosis of adenoid cystic carcinoma-2 cells induced by arsenic trioxide[J]. West China journal of stomatology, 2010, 28(3): 246-249. DOI: 10.3969/j.issn.1000-1182.2010.03.005 |
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| Authors: | ZHANG Bin MU Hai-bin XU Xu-guang LIU Wei HU Na-ri-su |
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| Affiliation: | 1. School of Municipal and Enviromental,Harbin Institute of Technology, Harbin 150090, China; 2. Dept. of Oral and Maxillofacial Surgery, The 2nd Clinical College, Harbin Medical University, Harbin 150086, China; 3. Clinical Diagnosis Center, The 2nd Clinical College, Harbin Medical University, Harbin 150086, China; 4. Dept. of Oral Implant, The 2nd Clinical College, Harbin Medical University,Harbin 150086, China |
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| Abstract: | Objective To investigate the proliferation effects of arsenic trioxide（As2O3） on salivary adenoid cystic carcinma-2（ACC-2） cells in vitro and to study the role of Survivin on the apoptosis of ACC-2 induced by As2O3. Methods ACC-2 cells were treated with different concentration of As2O3 for different time. The inhibitory effects on cell′s viability were assayed with methyl thiazolyl tetrazolium（MTT） test. Apoptosis was determined by flow cytometry. The expression of Survivin mRNA and protein were investigated by reverse transcription -polymerase chain raction （RT-PCR） and Western blot analysis respectively. Results Cell viability after As2O3 treatment was markedly suppressed and exhibited as a dose- and time-dependent pattern. The apoptotic index showed the similar trend. The results of RT-PCR revealed gene expression of Survivin was suppressed significantly. Through Western blot analysis, a negative correlation between concentration and amount of protein product of Survivin was determined. Conclusion As2O3 might markedly suppressed ACC-2 cell′s viability in vitro. The inhibition of Survivin gene expression may play a critical role on ACC-2 cell apoptosis induced by As2O3. |
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| Keywords: | arsenic trioxide adenoid cystic carcinoma apoptosis |
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