Effect of in vitro selenium supplementation on sperm quality in asthenoteratozoospermic men |
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| Authors: | A. A. Ghafarizadeh G. Vaezi M. A. Shariatzadeh A. A. Malekirad |
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| Affiliation: | 1. Department of Biology, Faculty of Science, Damghan Branch, Islamic Azad University, Damghan, Iran;2. Infertility Center, Iranian Academic Center for Education, Culture & Research (ACECR), Arak, Iran;3. Department of Biology, Faculty of Science, Science and Research Branch, Islamic Azad University, Tehran, Iran;4. Department of Biology, Faculty of Science, Arak University, Arak, Iran;5. Department of Biology, Payam Noor University, Tehran, Iran;6. Toxicology and Diseases Group, Pharmaceutical Sciences Research Center, Tehran University of Medical Sciences, Tehran, Iran |
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| Abstract: | Sperm DNA damage, excessive oxidative stress and decrease in motility ?may lead to low fertilisation or poor? assisted reproductive techniques outcomes in asthenoteratozoospermic ?men. Selenium was considered as essential element for male reproductive functions. Selenium has important role in enzymatic process for elimination of excessive reactive oxygen species and helps to maintain membrane integrity. The aim of this study was to determine the effect of selenium supplementation on sperm quality, DNA fragmentation, mitochondrial membrane potential and membrane lipid peroxidation during sperm sampling in vitro at different times. In this experimental study, semen samples were collected from 50 asthenoteratozoospermic men. Samples were divided into two groups as control group and test group (incubated with 2 μg/ml selenium at 37°C for 2, 4 and 6 hr). Motility and viability were assessed based on WHO 2010 criteria. Mitochondrial membrane potential, sperm DNA fragmentation and malondialdehyde levels were evaluated in each group. Results revealed that motility, viability and mitochondrial membrane potential were significantly higher in the test group (p < .05). Also malondialdehyde levels were significantly lower in the test group (p < .03). DNA fragmentation significantly decreased in the test group after 6 hr of incubation (p < .02). In conclusion, in vitro selenium supplementation may protect spermatozoa from maltreatment effect of reactive oxygen species (ROS) during sperm sampling via keeping enzymatic and antioxidant process in optimum condition. |
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| Keywords: | asthenoteratozoospermia DNA fragmentation reactive oxygen species selenium spermatozoa |
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