| CRISPR/Cas系统在病原体核酸检测中的应用进展 |
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| 引用本文： | 李梦茹,秦志强,殷堃,郑彬. CRISPR/Cas系统在病原体核酸检测中的应用进展[J]. 中国血吸虫病防治杂志, 2023, 35(1): 98-103+110 |
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| 作者姓名： | 李梦茹 秦志强 殷堃 郑彬 |
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| 作者单位： | 1 中国疾病预防控制中心寄生虫病预防控制所（国家热带病研究中心）、国家卫生健康委员会寄生虫病原与媒介生物学重点实验室、WHO热带病合作中心、科技部国家级热带病国际研究中心、上海交通大学医学院⁃国家热带病研究中心全球健康学院（上海 200025）； 2 上海交通大学医学院 |
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| 基金项目： | 国家重点研发计划项目（2021YFC2300800, 2021YFC2300803，2021YFC2300804） |
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| 摘 要： | 快速、灵敏、特异的检测方法对于传染病防控至关重要。聚合酶链式反应、等温扩增等体外核酸扩增技术已广泛应用于病原体检测。近年来,基于规则成簇的间隔短回文重复序列及其相关蛋白(CRISPR/Cas)系统的核酸检测方法显示出快速、高灵敏度、高特异性与便携性等优点。本文对CRISPR/Cas系统类型、原理及其在病原体检测中的应用进展进行综述,并对其应用前景进行展望。
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| 关 键 词： | CRISPR/Cas系统 核酸检测 病毒 细菌 寄生虫 |
Application of CRISPR/Cas systems in the nucleic acid detection of pathogens: a review |
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| LI Mengru,QIN Zhiqiang,YIN Kun,ZHENG Bin. Application of CRISPR/Cas systems in the nucleic acid detection of pathogens: a review[J]. Chinese journal of schistosomiasis control, 2023, 35(1): 98-103+110 |
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| Authors: | LI Mengru QIN Zhiqiang YIN Kun ZHENG Bin |
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| Abstract: | Rapid, sensitive and specific detection tools are critical for the prevention and control of infectious diseases. The in vitro nucleic acid amplification assays, including polymerase chain reaction and isothermal amplification technology, have been widely used for the detection of pathogens. Recently, nucleic acid detection⁃based on clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR⁃associated proteins (Cas) have been developed, which are rapid, highly sensitive, highly specific, and portable. This review describes the classification and principle of CRISPR/Cas systems and their applications in pathogen detection, and discusses the prospects of CRISPR/Cas systems. |
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| Keywords: | CRISPR/Cas system Nucleic acid detection Virus Bacterium Parasite |
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