| HPLC同时测定化妆品中22种香豆素类化合物及质谱确证 |
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| 引用本文： | 王红梅, 张燕惠, 李玲玲. HPLC同时测定化妆品中22种香豆素类化合物及质谱确证[J]. 中国现代应用药学, 2023, 40(1): 107-111. DOI: 10.13748/j.cnki.issn1007-7693.2023.01.014 |
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| 作者姓名： | 王红梅 张燕惠 李玲玲 |
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| 作者单位： | 1.厦门市食品药品质量检验研究院, 福建 厦门 361012 |
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| 摘 要： | 目的 建立同时测定化妆品中22种香豆素类化合物的HPLC及液相色谱-串联质谱验证方法。方法 采用Agilent Eclipse XDB-Phenyl色谱柱(150mm×4.6mm,3.5μm),流动相为乙腈-0.1%磷酸,梯度洗脱,流速为1.0mL·min-1,柱温为35℃,检测波长分别为227,310nm。以HPLC结果进行定性筛查,并采用标准曲线法定量,以液相色谱-串联质谱法的结果进行确证。结果 22种香豆素类化合物的线性范围为0.1~10mg·L-1,相关系数均>0.9993,方法检出浓度为0.1~1.5mg·kg-1,在低、中、高3种加标水平下,平均回收率为91.4%~114.8%,RSD为0.1%~4.8%。结论 该方法操作简便、准确,适用于膏乳、水剂、粉类及精油类化妆品中22种香豆素类化合物的检测。
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| 关 键 词： | 化妆品 高效液相色谱法 液相色谱-串联质谱法 香豆素类化合物 |
| 收稿时间： | 2021-11-23 |
Simultaneous Determination of 22 Coumarins in Cosmetics by HPLC and Verification by Mass Spectrometry |
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| WANG Hong-mei, ZHANG Yan-hui, LI Ling-ling. Simultaneous Determination of 22 Coumarins in Cosmetics by HPLC and Verification by Mass Spectrometry[J]. Chinese Journal of Modern Applied Pharmacy, 2023, 40(1): 107-111. DOI: 10.13748/j.cnki.issn1007-7693.2023.01.014 |
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| Authors: | WANG Hong-mei ZHANG Yan-hui LI Ling-ling |
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| Affiliation: | 1.Xiamen Institute for Food and Drug Control, Xiamen 361012, China |
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| Abstract: | OBJECTIVE To establish a method for the simultaneous determination of 22 coumarins in cosmetics by HPLC and verification by LC-MS/MS.METHODS Agilent Eclipse XDB-Phenyl chromatographic column(150 mm×4.6 mm, 3.5 μm)was used for gradient elution at a flow rate of 1.0 mL·min-1 with acetonitrile-0.1% phosphoric acid as the mobile phases. The column temperature was set at 35 ℃. The wavelengths of detection were 227, 310 nm. The results of HPLC were used for qualitative screening, the standard curve method was used for quantification, and the results of LC-MS/MS were used for verification.RESULTS The linear ranges of the 22 coumarins were 0.1-10 mg·L-1, and the correlation coefficients were >0.999 3.The detection limits were 0.1-1.5 mg·kg-1. The average recoveriese were 91.4%-114.8% with RSDs of 0.1%-4.8% at low, medium and high spiked levels.CONCLUSION The method is simple, accurate and suitable for the detection of 22 coumarins in creams,lotions, powdery sample and essential oil cosmetics. |
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| Keywords: | cosmetics HPLC LC-MS/MS coumarins |
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