| 蒿甲醚引起线粒体跨膜电位下降在神经毒性中的作用 |
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| 引用本文： | 赵艳红,王京燕. 蒿甲醚引起线粒体跨膜电位下降在神经毒性中的作用[J]. 中国寄生虫学与寄生虫病杂志, 2003, 21(6): 326-329 |
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| 作者姓名： | 赵艳红 王京燕 |
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| 作者单位： | 军事医学科学院微生物流行病研究所,北京,100071 |
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| 基金项目： | 国家自然科学基金课题 (No.3980 0 1 1 9)~~ |
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| 摘 要： | 目的 用嗜铬细胞瘤细胞(PC12细胞)、原代培养的大鼠神经元细胞和大鼠脑线粒体作为体外模型观察线粒体跨膜电位(mitochondrialmembranepotential,MMP)和细胞膜通透性改变在蒿甲醚引起的神经毒性中的作用。 方法 用流式细胞仪测定蒿甲醚对线粒体跨膜电位和细胞膜通透性的影响,用分光光度法分析蒿甲醚对线粒体肿胀度的影响。 结果 蒿甲醚能够降低两种细胞的MMP,使其峰值左移,摄入的Rh123荧光强度降低,量效关系明显;能增加两种细胞的细胞膜通透性,使细胞内的PI摄入增加;能引起大鼠脑线粒体肿胀,有一定的时间效应和剂量效应关系。结论 MMP下降是蒿甲醚引起神经毒性的重要环节,可通过影响线粒体膜通透性转运孔,改变线粒体跨膜电位和线粒体肿胀度,并能增加细胞膜通透性,引起细胞能量代谢障碍,最终导致神经毒性
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| 关 键 词： | 蒿甲醚 线粒体 MMP 膜通透性 膜肿胀 |
| 文章编号： | 1000-7423(2003)-06-0326-04 |
| 修稿时间： | 2002-12-23 |
Effect of Mitochondrial Membrane Potential in the Neurotoxicity of Artemether |
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| ZHAO Yan-hong,WANG Jing-yan. Effect of Mitochondrial Membrane Potential in the Neurotoxicity of Artemether[J]. Chinese Journal of Parasitology and Parasitic Diseases, 2003, 21(6): 326-329 |
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| Authors: | ZHAO Yan-hong WANG Jing-yan |
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| Affiliation: | Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, Beijing 100071. |
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| Abstract: | Objective To study the effect of mitochondrial membrane potential and cellular membrane permeability in the neurotoxicity of artemether. Methods Mitochondrial membrane potential and cellular membrane permeability in pheochromocytoma cell and primary cultured rat neuronal cell were measured by flow cytometry, using rhodamine 123(Rh123) and PI as fluorescent dye, respectively. Mitochondrial swelling was measured by spectrometer. Results Artemether could decrease the mitochondrial membrane potential and increase the cellular membrane permeability in both cell types in a dose-dependent manner. In addition, artemether led to mitochondrial swelling with dose-effect and time-effect relationships. Conclusion The decrease of mitochondrial membrane potential plays an important role in the neurotoxicity of artemether. The drug can change the mitochondrial membrane potential and mitochondrial swelling by affecting the permeability transition pore complex located in the mitochondrial membrane, increasing cellular membrane permeability, causing an obstruction and finally leading to neurotoxicity. |
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| Keywords: | artemether mitochondria mitochondrial membrane potential membrane permeability membrane swelling |
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