Expression cloning of the murine and human interleukin 9 receptor cDNAs. |
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| Authors: | J C Renauld C Druez A Kermouni F Houssiau C Uyttenhove E Van Roost J Van Snick |
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| Affiliation: | Ludwig Institute for Cancer Research, University of Louvain, Brussels, Belgium. |
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| Abstract: | Interleukin 9 (IL-9) is a T-cell-derived lymphokine that induces the proliferation of various lymphoid and hemopoietic cells. A cDNA clone encoding the murine IL-9 receptor was isolated by expression cloning in COS cells and screening with 125I-labeled IL-9. Transient expression of this cDNA produced high-affinity binding sites for IL-9. The predicted 52-kDa protein contains a putative signal peptide and a typical transmembrane domain. A cDNA for the human homologue was isolated by cross-hybridization. Transfection of this cDNA in a murine T-cell clone conferred responsiveness to human IL-9. Sequence analysis revealed that the IL-9 receptor belongs to the recently described hematopoietin receptor super-family and is expressed in membrane-bound and soluble forms. |
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