| LIT对小鼠抗弓形虫感染的保护作用 |
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| 引用本文： | 林爱芬,陆绍红,陈彩华,李思温,陈睿,陆漩辉,黄富泉. LIT对小鼠抗弓形虫感染的保护作用[J]. 中国寄生虫学与寄生虫病杂志, 1999, 17(6): 387-389 |
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| 作者姓名： | 林爱芬 陆绍红 陈彩华 李思温 陈睿 陆漩辉 黄富泉 |
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| 作者单位： | 浙江省医学科学院寄生虫病研究所!杭州310013(林爱芬，陆绍红，陈彩华，李思温，陈睿)，杭州大学物理系!杭州310028(陆漩辉)，杭州大学物理系!杭州(黄富泉) |
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| 摘 要： | 目的： 探讨激光照射弓形虫速殖子（ＬＩＴ） 的免疫保护力及其诱导的免疫应答水平。方法： 应用４．５ ＷＬＩＴ免疫ＩＣＲ小鼠，并用活体弓形虫速殖子攻击感染，观察ＬＩＴ抗虫效果。分别用间接免疫荧光法和ＥＬＩＳＡ法测定Ｔ淋巴细胞ＣＤ４＋ ／ＣＤ８＋ 亚群及特异性ＩｇＧ抗体。结果： ＬＩＴ免疫的小鼠具有抗急性弓形虫感染的能力， 能延长小鼠生存时间， 并能导致机体ＣＤ４＋ 、ＣＤ４＋ ／ＣＤ８＋ 显著升高及特异性ＩｇＧ抗体产生。结论： ４．５ Ｗ ＬＩＴ经腹腔免疫接种小鼠， 能诱导部分的保护性免疫力， 并能特异性地刺激小鼠产生体液免疫和细胞免疫应答
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| 关 键 词： | 激光照射 弓形虫速殖子 免疫应答 |
THE PROTECTIVE EFFECT AGAINST TOXOPLASMA INFECTION IN MICE IMMUNIZED WITH LASER-IRRADIATED TOXOPLASMA TACHYZOITES* |
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| LIN Aifen,LU Shaohong,CHEN Caihua,LI Siwen,CHEN Rui,LU Xuanhui,HUANG Fuquan. THE PROTECTIVE EFFECT AGAINST TOXOPLASMA INFECTION IN MICE IMMUNIZED WITH LASER-IRRADIATED TOXOPLASMA TACHYZOITES*[J]. Chinese Journal of Parasitology and Parasitic Diseases, 1999, 17(6): 387-389 |
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| Authors: | LIN Aifen LU Shaohong CHEN Caihua LI Siwen CHEN Rui LU Xuanhui HUANG Fuquan |
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| Affiliation: | Institute of Parasitic Diseases, Zhejiang Academy of Medical Sciences, Hangzhou 310013. |
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| Abstract: | AIM: To observe the level of immune responses and protective immunity in mice induced by laser|irradiated Toxoplasma tachyzoites (LIT).METHODS: ICR mice were immunized with LIT. After one month, the mice were challenged with live Toxoplasma tachyzoites to observe the effect of LIT. Indirect immunofluorescence assay and ELISA were used to detect CD4 /CD8 lymphocyte subpopulation and IgG antibody. RESULTS: Immunization with LIT could partially protect the mice from Toxoplasma infection, prolong the survival time, enhance the CD4 /CD8 ratio and produce specific IgG antibody. CONCLUSION: Intraperitoneal injection with LIT can induce partial protective immunity and specifically elicit ICR mice to generate humoral and cell|mediated immune responses.; |
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| Keywords: | Laser|irradiation Toxoplasma tachyzoite immune response |
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