| 层粘连蛋白靶向NT3促进创伤性脑损伤的修复研究 |
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| 引用本文： | 樊娟,张振海,张国良,沈春森,罗永春,袁晓敏,张鹏飞,党圆圆,徐如祥. 层粘连蛋白靶向NT3促进创伤性脑损伤的修复研究[J]. 中华神经创伤外科电子杂志, 2016, 2(1): 26-30. DOI: 10.3877/cma.j.issn.2095-9141.2016.01.007 |
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| 作者姓名： | 樊娟 张振海 张国良 沈春森 罗永春 袁晓敏 张鹏飞 党圆圆 徐如祥 |
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| 作者单位： | 1. 100700 北京，北京军区总医院神经外科研究所 |
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| 基金项目： | 国自然基金青年项目(81200959) |
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| 摘 要： | 目的研制能与层粘连蛋白牢固结合的含有层粘连蛋白(laminin)结合域的NT3(LBD-NT3)，探讨其与损伤后增多的层粘连蛋白结合促进脑损伤的修复机理。 方法46只SD大鼠随机分为空白对照组(8只)、假手术组(8只)、PBS组(10只)、NT3组(10只)、LBD-NT3组(10只)，制作液压打击模型后注射对应的PBS或因子，利用水迷宫实验进行行为学评分，1个月后处死大鼠，通过大鼠脑组织免疫荧光染色，比较各组神经元和胶质细胞的增减，用SPSS 19.0统计学做统计分析。 结果LBD-NT3组大鼠的水迷宫实验表现更好，和假手术组大鼠相比，P=0.845>0.05；与空白对照组相比，P=0.956>0.05；与PBS相比，P=0.006<0.05；与NT3组相比，P=0.089>0.05。笔者进一步得出，第17、18天，NT3组大鼠和LBD-NT3组有显著性差异，P<0.05。神经元细胞较多，胶质细胞较少，LBD-NT3与PBS组、NT3组差异具有统计学意义（P<0.05），与空白对照组、假手术组未见统计学差异。 结论LBD-NT3和体内增多的laminin结合后，大鼠脑组织损伤相对较小，周围的神经元较多，胶质细胞较少，功能保留较好。
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| 关 键 词： | 层粘连蛋白 创伤性脑损伤 神经营养因子-3 |
| 收稿时间： | 2015-12-02 |
Laminin targeted NT3 promotes traumatic brain injury repair |
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| Juan Fan,Zhenhai Zhang,Guoliang Zhang,Chunsen Shen,Yongchun Luo,Xiaomin Yuan,Pengfei Zhang,Yuanyuan Dang. Laminin targeted NT3 promotes traumatic brain injury repair[J]. Chinese Journal of Neurotraumatic Surgery, 2016, 2(1): 26-30. DOI: 10.3877/cma.j.issn.2095-9141.2016.01.007 |
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| Authors: | Juan Fan Zhenhai Zhang Guoliang Zhang Chunsen Shen Yongchun Luo Xiaomin Yuan Pengfei Zhang Yuanyuan Dang |
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| Affiliation: | 1. Department of Neurosurgery Research Institute, The Military General Hospital of Beijing PLA, 100700 Beijing, China |
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| Abstract: | ObjectiveTo construct and prepare laminin binding domain NT3(LBD-NT3), exploring its mechanism to promote brain injury repaire with increasing laminin. MethodsFourty-six rats were grouped into naive(8)、sham(8)、PBS(10)、NT3(10)、LBD-NT3(10) randomly. Following hydraulic strike, PBS or NT3 or LBD-NT3 was injected into the lesion. Behavioral test went on by the Morris water maze task. One month after the operation, the rats were perfused and executed, neuron and glial cell were compared by NeuN and GFAP separately with Immunofluorescence staining. Statistical analysis was made by SPSS 19.0. ResultsIn the Laminin-NT3 group, rats performed better in Morris water maze task, P<0.05(compared with PBS), P>0.05(compared with na?ve、sham、NT3), but significantly better than NT3 group on 17th、18th day (P<0.05) . LBD-NT3 had more positive NeuN cells and and less GFAP expression than PBS、NT3 (P<0.05) . There was statistical significance among the groups. ConclusionLBD-NT3 could bind to the increasing laminin. Therefore, the brain lesion was smaller, neurons increased and glial cells decreased. |
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| Keywords: | Laminin Traumatic brain injury Neurotrophin-3 |
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