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| 大鼠侧脑室注射Apelin-13对急性痛的调节作用及机制 | |
| 引用本文： | 徐震,王庆尧,王贤斌,武菲. 大鼠侧脑室注射Apelin-13对急性痛的调节作用及机制[J]. 中华诊断学电子杂志, 2016, 4(4): 279-283. DOI: 10.3877/cma.j.issn.2095-655X.2016.04.017 |
| 作者姓名： | 徐震 王庆尧 王贤斌 武菲 |
| 作者单位： | 1. 272067 济宁医学院神经生物学研究所 |
| 基金项目： | 2016年地方高校国家级大学生创新创业训练计划项目(201610443048); 济宁医学院青年基金(JYQ14KJ19); 2015年度济宁市医药卫生计划项目(79); 济宁医学院大学生创新训练计划项目(CX2015005) |
| 摘 要： | 目的探讨Apelin-13对大鼠急性痛的调制作用及可能机制。 方法简单随机抽样将50只大鼠分为生理盐水组、Apelin-13 0.05μg/g组、Apelin-13 0.1μg/g组、Apelin-13 0.5μg/g组及吗啡组(阳性对照组)，每组10只，采用辐射热甩尾法连续监测60 min内大鼠痛阈的变化；同样采用简单随机抽样将40只大鼠分为生理盐水组、Apelin-13 0.5 μg/g组、酪蛋白激酶2(CK2)抑制剂四溴苯三唑(TBB)组及Apelin-13＋TBB组，每组10只，监测其痛阈的变化。Western Blot法检测海马内CK2表达量及N-甲基-D-天冬氨酸(NMDA)受体NR2B亚单位(P-NR2B S1480)磷酸化水平，研究Apelin－13对急性痛调制作用可能的机制。 结果Apelin-13可显著升高急性痛痛阈，0.5 μg/g组给药10 min时甩尾潜伏期变化率(TWL%)达峰值[(50.03±2.71)%]，与生理盐水组比较差异有统计学意义(q＝18.054，P＝0.003)，并且此效应呈剂量和时间依赖(FGroup＝47.729，P＝0.000；FTime＝205.301，P＝0.000)。同时给予CK2抑制剂TBB10 min时TWL%降至(17.75±1.67)%，低于Apelin-13组，差异有统计学意义(q＝6.976，P＝0.005)。Western Blot结果显示TBB可明显减弱Apelin-13的作用，CK2α表达量、NR2B亚单位磷酸化水平均下降[(26.92±4.38)%，(39.90±7.40)%]，与Apelin-13组[(41.60±6.65)%，(70.83±3.52)%]比较差异有统计学意义(q＝5.246，P＝0.010；q＝9.757，P＝0.010)。 结论Apelin-13可能通过CK2促进NMDA受体NR2B亚单位磷酸化，进而在热甩尾急性痛模型中起镇痛作用。 |
| 关 键 词： | Apelin 急性痛 镇痛 酪蛋白激酶Ⅱ NR2B亚单位 |
| 收稿时间： | 2016-06-13 |
The modulation and mechanism of Apelin-13 in an acute pain model |
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| Zhen Xu,Qingyao Wang,Xianbin Wang,Fei Wu. The modulation and mechanism of Apelin-13 in an acute pain model[J]. Chinese Journal of Diagnostics (Electronic Edition), 2016, 4(4): 279-283. DOI: 10.3877/cma.j.issn.2095-655X.2016.04.017 | |
| Authors: | Zhen Xu Qingyao Wang Xianbin Wang Fei Wu |
| Affiliation: | 1. Institute of Neurobiology, Jining Medical University, Jining 272067, China |
| Abstract: | ObjectiveTo explore the modulation and mechanism of Apelin-13 in an acute pain model. MethodsThe pain thresholds were continuously monitored in 60 min after intracerebroventricular (ICV) administration of Apelin-13 (0.05, 0.1 and 0.5 μg/g) and casein kinase 2(CK2) inhibitor 4, 5, 6, 7-tetrabromoben: zotriazole (TBB) by radiant heat tail-flick test.Then the expression of CK2 and phosphorylation of NR2B S1480 in hippocampus were detected by Western Blot. ResultsICV injection of Apelin-13 induced a dose and time-dependent antinociceptive effect.This effect was signicantly antagonized by CK2 inhibitor TBB.Western Blot showed that Apelin-13 could significantly promote the expression of CK2 and the phosphorylation of NR2B S1480, which decreased obviously by TBB. ConclusionApelin-13 plays an antinociceptive role in the acute pain model of radiant heat tail-flick test via modulating CK2 expression and the phosphorylation of NR2B S1480. |
| Keywords: | Apelin Acute pain Analgesia Casein kinase Ⅱ NR2B |
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