| CPP32在阿霉素诱导的胃癌耐药细胞的凋亡的作用 |
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| 引用本文： | 王少东,张振书,张杏泉,周殿元. CPP32在阿霉素诱导的胃癌耐药细胞的凋亡的作用[J]. 世界华人消化杂志, 2001, 9(10): 1152-1156 |
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| 作者姓名： | 王少东 张振书 张杏泉 周殿元 |
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| 作者单位： | 1. 第一军医大学南方医院消化内科,
2. 第四军医大学唐都医院骨科医院, |
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| 摘 要： |
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| 关 键 词： | 胃肿瘤/病理学 caspase类/代谢 抗药性,多药 抗药性,肿瘤 凋亡 |
| 修稿时间： | 2001-03-28 |
The function of CPP32 in apoptosis of multidrug-resistance gastric cancer cells |
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| Shao-Dong Wang Zheng-Shu Zhang Xing-Quan Zhang Dian-Yuan ZhouCorrespondence to:Shao-Dong Wang. The function of CPP32 in apoptosis of multidrug-resistance gastric cancer cells[J]. World Chinese Journal of Digestology, 2001, 9(10): 1152-1156 |
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| Authors: | Shao-Dong Wang Zheng-Shu Zhang Xing-Quan Zhang Dian-Yuan ZhouCorrespondence to:Shao-Dong Wang |
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| Affiliation: | Shao-Dong Wang Zheng-Shu Zhang Xing-Quan Zhang Dian-Yuan ZhouCorrespondence to:Shao-Dong Wang |
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| Abstract: | AIM To identify the effect and the activity of CPP32 in multidrug resistance cell by comparing the gane expression,protein expression,and the relationship between them.We try to illuminate the effect and the significance of cespees-3 in the apoptosis induced by cheomotherapeutic agents in multidrug resistance cells. METHODS Utilizing the established multidrug resistance cell line,we investigated the mRNA expression of caspese-3 in multidrug resistance cells at Oh,24h;and in drug sensitive cells at 0h,6h by RT-PCR.We determined the protein expression of in multidrug resistance cells at 0h,6h,24h,30h;and in drug sensitive cells at 0h,6h,24h by westem blot.DNA ladder was used to examine the characteristic changes of apeptosis,MTT was performed to get survival rate of cells. RESULTS there are no significant difference between the mRNA expression of the resistant cells at 0h,24h and that of the drug sensitive cells at 0h,6h.Analysis of protein expression showed that there is trace expression in resistant cells at 6h;the small degradation products appears at 30h;the little degradation products appears at 6h in sensitive cells,the cespase-3 protein is splitted completely at 24h.DNA analysis show that the typical apoptotic ladder at 30h in resistant cells;the apoptosis of resistant cells was delayed after the inhibitor was applied.The same was occurred at 6h in sensitive cells. CONCLUSIONS Our results imply that execution of apoptosis is the same in mdr cells and sensitive cells,the only difference between mdr cell and sensitive cell is the concentration of the drug and the time of caspess-3 when they have their effect. |
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| Keywords: | stomach neoplasms/pathology caspases/metabolism drug resistance multiple drug resistance neoplasm apoptosis |
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