| 在线SPE HPLC-DAD方法测定大鼠血浆环磷酰胺浓度(英文) |
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| 引用本文： | 李晓娜,于宁,张建美,林文斯,凌笑梅,富戈,李润涛,崔景荣. 在线SPE HPLC-DAD方法测定大鼠血浆环磷酰胺浓度(英文)[J]. 中国药学, 2012, 21(2): 156-161. DOI: 10.5246/jcps.2012.02.020 |
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| 作者姓名： | 李晓娜 于宁 张建美 林文斯 凌笑梅 富戈 李润涛 崔景荣 |
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| 作者单位： | 北京大学医学部药物分析教研室;北京大学医学部天然药物及仿生药物国家重点实验室 |
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| 基金项目： | National Natural Science Foundation of China(Grant No.81072612);the Natural Science Foundation of Beijing(Grant No.7102107);the Open Foundation of State Key Laboratory of Natural and Biomimetic Drugs(Grant No.K20110109);Specialized Research Fund for the Doctoral Program of Higher Education(Grant No.20110001110021) |
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| 摘 要： | 建立快速简便的在线固相萃取液相(on-line SPE HPLC-DAD)分析方法,测定大鼠血浆中环磷酰胺的浓度。该方法为:将Acclaim Polar Advantage II C18保护柱(PA II柱,10mm×4.6mm,5μm)作为在线SPE柱,去蛋白的血浆样品进样后,100%H2O洗脱1min;经PA II C18柱(150mm×4.6mm,5μm)分析,流动相为乙腈水(40:60,v/v),流速为1mL/min,检测波长为195nm。经过验证发现该方法的选择性好,线性、精密度、准确度和灵敏度高,可用于临床监测环磷酰胺浓度。
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| 关 键 词： | 在线SPE HPLC-DAD 环磷酰胺 大鼠血浆 |
Quantitative determination of cyclophosphamide in rat plasma using an on-line SPE HPLC-DAD |
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| Xiao-Na Li,Ning Yu,Jian-Mei Zhang,Wen-Si Lin,Xiao-Mei Ling,Ge Fu,Run-Tao Li,Jing-Rong Cui. Quantitative determination of cyclophosphamide in rat plasma using an on-line SPE HPLC-DAD[J]. Journal of Chinese Pharmaceutical Sciences, 2012, 21(2): 156-161. DOI: 10.5246/jcps.2012.02.020 |
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| Authors: | Xiao-Na Li Ning Yu Jian-Mei Zhang Wen-Si Lin Xiao-Mei Ling Ge Fu Run-Tao Li Jing-Rong Cui |
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| Affiliation: | 1.Department of Pharmaceutical Analysis,School of Pharmaceutical Sciences,Peking University,Beijing 100191,China 2.State Key Laboratory of Natural and Biomimetic Drugs,Peking University Health Science Center,Beijing 100191,China |
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| Abstract: | A rapid and simple liquid chromatography method with on-line solid phase extraction was developed and validated for the quantitative determination of cyclophosphamide in rat plasma.The plasma sample was first extracted on an Acclaim? Polar Advantage II C18 guard column(PA II C18,10 mm×4.6 mm,5 μm),which was also the on-line Extraction Cartridge SPE column,by washing with 100% H2O for 1 min.The extracted sample was then eluted onto a PA II C18 column(150 mm×4.6 mm,5 μm) and separated by isocratic elution with acetonitrile-water(40:60,v/v).The mobile phase was run at a flow rate of 1.0 mL/min,and the UV detector was set at 195 nm.Retention time of cyclophosphamide was 4.3 min and the total run-time was 6 min.The linear range of the standard curve was from 1.0 to 200 μg/mL(r2 = 0.9999),and the limits of quantification and detection were 1.0 μg/mL(RSD<10%,n = 5) and 0.3 μg/mL(RSD<13%,n = 5),respectively.Both intra-and inter-day variations were less than 5.6%.The developed method can be used for the therapeutic drug monitoring of cyclophosphamide in the clinic. |
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| Keywords: | On-line SPE HPLC-DAD Cyclophosphamide Rat plasma |
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