| 猪囊尾蚴抗原cC1与γ-干扰素融合蛋白在大肠杆菌中的表达 |
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| 引用本文： | 郭瀛军,吴丹,孙树汉. 猪囊尾蚴抗原cC1与γ-干扰素融合蛋白在大肠杆菌中的表达[J]. 中国寄生虫学与寄生虫病杂志, 1999, 17(4): 215-217 |
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| 作者姓名： | 郭瀛军 吴丹 孙树汉 |
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| 作者单位： | 第二军医大学医学遗传学教研室 上海 200433; |
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| 摘 要： | 目的： 构建含猪囊尾蚴抗原ｃ Ｃ１ ｃ Ｄ Ｎ Ａ 与猪γ 干扰素（ Ｉ Ｆ Ｎ γ） 的融合表达载体。方法： 从已克隆的含人猪囊尾蚴共通抗原ｃ Ｃ１ 的质粒中, 用 Ｐ Ｃ Ｒ 方法扩增出含酶切位点及接头的ｃ Ｃ１ 和 Ｉ Ｆ Ｎ γｃ Ｄ Ｎ Ａ, 两ｃ Ｄ Ｎ Ａ 经接头融合后构建成融合形式的原核表达载体转入大肠杆菌 Ｘ Ｌ Ｂｌｕｅ。结果： 经酶切分析, 表明重组载体中含１５ｋｂ 插入片段。 Ｓ Ｄ Ｓ Ｐ Ａ Ｇ Ｅ 显示一５２ ｋ Ｄａ 的诱导产物。结论： 猪 Ｉ Ｆ Ｎ γ和猪囊尾蚴抗原ｃ Ｃ１ 融合ｃ Ｄ Ｎ Ａ 在大肠杆菌中获得表达。
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| 关 键 词： | 囊尾蚴病 融合蛋白 γ-干扰素 |
Fusion expression of porcine IFN-gamma and Cysticercus cellulosae antigen cC1 in Escherichia coli cells] |
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| GUO Yingjun,WU Dan,SUN Shuhan. Fusion expression of porcine IFN-gamma and Cysticercus cellulosae antigen cC1 in Escherichia coli cells][J]. Chinese Journal of Parasitology and Parasitic Diseases, 1999, 17(4): 215-217 |
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| Authors: | GUO Yingjun WU Dan SUN Shuhan |
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| Affiliation: | Department of Medical Genetics, Second Military Medical University, Shanghai 200433. |
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| Abstract: | AIM: To construct an expression vector, including a chimeric cDNA of porcine IFN-gamma and Cysticercus cellulosae antigen cC1. METHODS: DNA fragments of porcine IFN-gamma and cC1 including linkers were generated by polymerase chain reaction (PCR). The recombinant vector pJLA-PRcC1 was constructed by inserting a chimeric cDNA of porcine IFN-gamma and Cysticercus cellulosae antigen cC1, and transformed to E. coli XL-Blue. RESULTS: The recombinant vector was identified by restriction analysis. An inserted fragment about 1.5 kb could be found. After induction, a 52 kDa new protein band appeared in SDS-PAGE. CONCLUSION: The fusion expression of porcine IFN-gamma and cC1 antigen in Escherichia coli cells is successful. |
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| Keywords: | Cysticercosis')" >Cysticercosis fusion protein ')" >IFN-γ |
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