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Chemiluminescent hybridization assay for hepatitis delta virus RNA in serum. |
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| Authors: | M Gallagher T T Morris H A Fields |
| Abstract: | A nonradioactive hybridization assay for the detection of hepatitis delta virus RNA in serum is described. This assay utilizes a digoxigenin-labeled RNA hybridization probe and chemiluminescent immunodetection. The probe can detect as little as 0.4 pg of cDNA with a 60-min exposure. Results obtained are in agreement with serological indicators of hepatitis delta virus infection and are comparable to those obtained by hybridization assays employing radioactively labeled RNA. |
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