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Mast cell mediated ion transport in intestine from patients withand without inflammatory bowel disease |
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| Authors: | S Crowe G Luthra M Perdue |
| Affiliation: | Department of Internal Medicine, University of Texas Medical Branch, Galveston, USA. |
| Abstract: | Background—Mast cells have been shown to regulateintestinal ion transport in animal models and normal human colon buttheir physiological role in human intestinal inflammatory disorders is unknown. Aims—To examine mast cell regulation of iontransport in inflammatory bowel disease (IBD). Subjects and methods—Small and large intestinewas obtained from patients with and without IBD undergoing surgicalresection. Short circuit current (Isc) responses to rabbitantihuman IgE, histamine, and electrical stimulation were measured inUssing chambers. Specimens were also examined for mast cell numbers and degree of inflammation. Results—Isc responses to anti-IgEand histamine were smaller in magnitude in IBD compared with non-IBDtissues. In all tissues, anti-IgE Isc responses werereduced by about 80% in chloride free buffer. The histamineH1 receptor antagonist, pyrilamine, decreased anti-IgEresponses in non-IBD tissues. Greater inhibition with pyrilamine wasseen in IBD small intestine but its effect was less in IBD colon.Histamine pretreatment of non-IBD control tissues reduced anti-IgEresponses to levels seen in IBD colon but had no effect in smallintestine. Mast cell numbers were greater in IBD compared with non-IBDsmall intestine while no differences were observed between the colonicgroups. Isc responses to anti-IgE were not correlated withthe degree of mucosal inflammation. Conclusions—This study provides further evidencethat mast cells are capable of mediating alterations of ion transportin human gut but that this regulatory role may be altered in IBD. Thedata suggest that prior activation of mast cells with release ofhistamine may account for the reduced secretory response to anti-IgEobserved in IBD colonic tissues. Keywords:mast cells; intestine; ion transport; histamine; ulcerative colitis; Crohn's disease |
| Keywords: | mast cells intestine ion transport histamine ulcerative colitis Crohn''s disease |