New liposomal doxorubicin nanoformulation for osteosarcoma: Drug release kinetic study based on thermo and pH sensitivity |
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| Authors: | Fateme Haghiralsadat Ghasem Amoabediny Mohammad Hasan Sheikhha Behrouz Zandieh‐doulabi Samira Naderinezhad Marco N. Helder Tymour Forouzanfar |
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| Affiliation: | 1. Department of Life Science Engineering, Faculty of New Sciences & Technologies, University of Tehran, Tehran, Iran;2. Department of Nano Biotechnology, Research Center for New Technologies in Life Science Engineering, University of Tehran, Tehran, Iran;3. Department of Biotechnology and Pharmaceutical Engineering, School of Engineering, University of Tehran, Tehran, Iran;4. Department of Oral & Maxillofacial Surgery, VU University Medical Center, MOVE Research Institute Amsterdam, Amsterdam, The Netherlands;5. Research and Clinical Center for Infertility, Shahid Sadoughi University of Medical Sciences, Yazd, Iran;6. Biotechnology Research Center, International Campus, Shahid Sadoughi University of Medical Science, Yazd, Iran;7. Department of Orthopedic Surgery, VU University Medical Center, MOVE Research Institute, Amsterdam, Netherlands;8. Oral Cell Biology and Functional Anatomy, VU University, Amsterdam, North Holland, Netherlands |
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| Abstract: | A novel approach was developed for the preparation of stealth controlled‐release liposomal doxorubicin. Various liposomal formulations were prepared by employing both thin film and pH gradient hydration techniques. The optimum formulation contained phospholipid and cholesterol in 1:0.43 molar ratios in the presence of 3% DSPE ‐mPEG (2000). The liposomal formulation was evaluated by determining mean size of vesicle, encapsulation efficiency, polydispersity index, zeta potentials, carrier's functionalization, and surface morphology. The vesicle size, encapsulation efficiency, polydispersity index, and zeta potentials of purposed formula were 93.61 nm, 82.8%, 0.14, and ?23, respectively. Vesicles were round‐shaped and smooth‐surfaced entities with sharp boundaries. In addition, two colorimetric methods for cytotoxicity assay were compared and the IC 50 (the half maximal inhibitory concentration) of both methods for encapsulated doxorubicin was determined to be 0.1 μg/ml. The results of kinetic drug release were investigated at several different temperatures and pH levels, which showed that purposed formulation was thermo and pH sensitive. |
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| Keywords: | cytotoxicity drug delivery liposome characterization osteosarcoma release kinetics |
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