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| 体外冲击波对兔膝软骨细胞增殖的影响 | |
| 引用本文： | 赵喆,刘春梅,白晓东,邢更彦. 体外冲击波对兔膝软骨细胞增殖的影响[J]. 中国康复理论与实践, 2012, 18(9): 824-826 |
| 作者姓名： | 赵喆 刘春梅 白晓东 邢更彦 |
| 作者单位： | 1.武警总医院骨科，北京市100039；2.武警山西总队朔州支队，山西朔州市036002。 |
| 基金项目： | 国家自然科学基金项目(31172169) |
| 摘 要： | 目的体外观察体外冲击波(ESW)对软骨细胞增殖的影响。方法酶法消化获得正常兔膝软骨细胞，体外分别施加0、1×105、1.5×105、2×105、2.5×105、3×105、3.5×105 Pa ESW 0、300、600、900 次。倒置显微镜观察，HE 染色观察，CCK-8 染色筛选ESW促进软骨细胞增殖最佳压强和次数。结果1.5×105 Pa、600 次ESW干预后，软骨细胞增殖活性达最高；随压强、次数升高，软骨细胞增殖活性明显下降。结论软骨细胞是对ESW应力敏感细胞。ESW在一定压强和次数下显著促进软骨细胞增殖。 |
| 关 键 词： | 体外冲击波 软骨细胞 细胞增殖 应力细胞 |
| 收稿时间： | 2012-03-29 |
Effect of Extracorporeal Shock Wave on Chondrocyte Proliferation of Rabbit Knee in Vitro |
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| ZHAO Zhe,LIU Chun-mei,BAI Xiao-dong,et al. Effect of Extracorporeal Shock Wave on Chondrocyte Proliferation of Rabbit Knee in Vitro[J]. Chinese Journal of Rehabilitation Theory and Practice, 2012, 18(9): 824-826 | |
| Authors: | ZHAO Zhe LIU Chun-mei BAI Xiao-dong et al |
| Affiliation: | Department of Orthopaedic Surgery, General Hospital of Chinese People's Army Police Forces, Beijing 100039, China |
| Abstract: | Objective To observe the effect of extracorporeal shock wave(ESW) on proliferation of chondrocyte in vitro.Methods Chondrocyte from normal rabbit knee using enzymatic digestion was treated with ESW of 0,1×105,1.5×105,2×105,2.5×105,3×105,3.5×105 Pa 0,300,600,900 times in vitro.The pressure and frequency of ESW most promoting chondrocyte proliferation were determined with cartilage cell morphology,HE staining and CCK-8 assay.Results Chondrocyte proliferated most under 1.5×105 Pa and 600 times of ESW,and decreased as the pressure and the frequency increased.Conclusion Chondrocyte is stress-sensitive in vitro.ESW can promote chondrocyte proliferation in certain pressure and frequency. |
| Keywords: | extracorporeal shock wave chondrocyte proliferation stress cells |
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