Rapid detection of t(15;17)(q24;q21) in acute promyelocytic leukaemia by microwave‐assisted fluorescence in situ hybridization |
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| Authors: | Silvia Soriani Cinzia Mura Anna Rita Panico Anna Maria Scarpa Patrizia Recchimuzzo Raffaella Dadati Renata Farioli Gabriella De Canal Maria Angela Mura Clara Cesana |
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| Affiliation: | 1. Department of Laboratory Medicine, Niguarda Ca' Granda Hospital, Milan, Italy;2. Department of Hematology, Niguarda Ca' Granda Hospital, Milan, Italy |
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| Abstract: | Acute promyelocytic leukaemia (APL) is a hematologic malignancy characterized by the rearrangement of the PML and RARα genes, mostly due to a reciprocal chromosomal translocation t(15;17)(q24;q21). A quick APL diagnosis is essential for starting a prompt suitable therapy. We describe a new rapid diagnostic laboratory approach to detect the PML‐RARα rearrangement, which gives clear genetic results within 30 min of hybridization. It combines quick cell harvesting, fluorescence in situ hybridization performed with commercial DNA probe and microwave beams supplied by a domestic microwave oven. Copyright © 2015 John Wiley & Sons, Ltd. |
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| Keywords: | acute promyelocytic leukaemia fluorescence in situ hybridization microwave oven |
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