| 光照不均匀性对SD大鼠血液生理生化指标的影响 |
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| 引用本文： | 王德军,周卫民,王辉,寿旗扬,朱科燕,陈民利. 光照不均匀性对SD大鼠血液生理生化指标的影响[J]. 实验动物与比较医学, 2010, 30(4): 277-282. DOI: 10.3969/j.issn.1674-5817.2010.04.010 |
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| 作者姓名： | 王德军 周卫民 王辉 寿旗扬 朱科燕 陈民利 |
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| 作者单位： | 浙江中医药大学动物实验研究中心/比较医学研究中心,杭州,310053 |
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| 基金项目： | 浙江省实验动物科技计划项目,浙江省卫生高层次人才工程项目 |
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| 摘 要： | 目的探讨光照的不均匀性对正常SD大鼠血液生理生化指标的影响。方法将体重60-80g的SD大鼠80只,适应饲养一周后,进行实验饲养模拟,饲养笼位从上到下分为A、B、c、D层,测定笼位内不同点的光照度及其所占面积,每2周测采食量和体重,8周后测定血常规和血液生化指标。结果（1）笼位内侧光照度A、B、c、D层无显著差异（P〉0．05）,各层笼位外侧和中部的光照度及其所占笼位面积有显著性差异（P〈0．01,P〈0．05）;（2）A—D层大鼠的采食量和体重均无明显差异（P〉0．05）：（3）A至D层雌性大鼠WBC、MCHC、HDW、MPV、NEUT、EOS、BASO和Retic均有不同程度的升高或降低（P〈0．05,P〈0．01）;（4）A至D层雌性大鼠AsT、TBIL、UA、TG、CK、CK—MB有明显降低,ALP、GLu明显升高（P〈0．05,P〈0．01）：而雄性大鼠只有TBIL、CK-MB有明显降低,GLU、BUN有明显升高（JP〈0．05,P〈0．01）。（5）同层大鼠雌性与雄性相比,部分血液生理和生化指标存在显著差异（P〈0．05,P〈0．01）。结论不同层的笼位光照具有不均匀性,且对SD大鼠的血液生理生化指标有一定的影响,尤对雌性大鼠的影响更明显。
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| 关 键 词： | 光照不均匀性 SD大鼠 血液生理生化 |
Effect of Lighting Asymmetry on Blood Physiological and Biochemical Indexes in SD Rat |
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| WANG De-jun,ZHOU Wei-min,WANG Hui,SHOU Qi-yang,ZHU Ke-yan,CHEN Min-li. Effect of Lighting Asymmetry on Blood Physiological and Biochemical Indexes in SD Rat[J]. Laboratory Animal and Comparative Medicine, 2010, 30(4): 277-282. DOI: 10.3969/j.issn.1674-5817.2010.04.010 |
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| Authors: | WANG De-jun ZHOU Wei-min WANG Hui SHOU Qi-yang ZHU Ke-yan CHEN Min-li |
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| Affiliation: | (Zhejiang Chinese Medical University Laboratory Animal Research Center/ Comparable Medical Research Ccenter, Hangzhou 310053, China) |
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| Abstract: | Objective To observe the effect of lighting asymmetry on the blood physiological and biochemical indexes of normal SD rats. Mothods Eighty SD rats, weighting 60-80 g, were kept on the rack with different layer （A,B,C,D） after one-week＇s adaption. Light-intensity and light area were measuerd in each cage. Food consumption and body weight were calculated every 2 weeks. Blood physiological and biochemical indexes were detected after 8 weeks. Results （1）The light-intensity among inner inside of four layer＇s cages was no significant difference （P〉0.05）, however, there is a significant difference light-intensity and cage-area in outside and median site of cages in each layer （P〈0.01, P〈0.05）. （2）The food consumption and weights of A and D layers rats have no obvious differences （P〉0.05）. （3）The blood physiology shows WBC, MCHC, HDW, MPV, NEUT, EOS, BASO and Retic of female rats from A to D layers varied at different degrees （P〈0.05, P〈0.01）. （4） The Blood Biochemistry shows AST, TBIL, UA, TG, CK, and CK-MB of female rats from A to D have obviously decreased, ALP and GLU have significantly increased （P 〈0.05, P〈0.01）; while TBIL and CK-MB of male rats were markedly decreased, GLU and BUN were notably enhanced （P〈0.05, P〈0.01）. （5） Female rats compared with the males on the same layer, some physiological and biochemical indicators of blood there were significant differences（P 〈0.05, P 〈0.01）. Conclusion The light asymmetry was in different layer of the cages can cause the change of blood biochemical indexes to a certain extent, and the change was more obvious in female rats. |
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| Keywords: | Lighting asymmetry SD rat Blood physiological and biochemistry |
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