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| 游离型HPV16细胞类型特异性增强子片段的变异对启动子活性的影响 | |
| 引用本文： | 李玲,;刘文康,;楚雍烈,;刘湘,;任健康. 游离型HPV16细胞类型特异性增强子片段的变异对启动子活性的影响[J]. 延安大学学报(医学科学版), 2014, 12(2): 1-4 |
| 作者姓名： | 李玲, 刘文康, 楚雍烈, 刘湘, 任健康 |
| 作者单位： | [1]陕西省人民医院检验科,陕西西安710068; [2]西安交通大学基础医学院病原生物系,陕西西安710061 |
| 基金项目： | 国家自然科学基金资助项目（NO.81272856） |
| 摘 要： | 目的 研究宫颈癌中游离型HPV16中细胞特异性增强子(cell-type specific enhancer,CTSE)片段的变异对启动子P97活性的影响,探讨CTSE片段变异与游离型HPV16致癌机制的关系。方法 分别构建含HPV16突变型及野生型CTSE片段的重组报道基因载体(pCAT3-Promoter)表达系统,利用脂质体将重组的报道基因载体转染HeLa细胞;ELISA法测定细胞中CAT蛋白表达量的变化,了解变异CTSE片段对启动子活性的影响。结果 与无增强子的CAT报道质粒pCAT3-Promoter相比,插入HPV16 CTSE 序列的pCAT3-Promoter重组报道质粒CAT蛋白表达量增加1.5～2.5倍;插入突变型CTSE 序列的报道载体与插入野生型CTSE 序列的报道载体相比,报道基因CAT蛋白表达量增加了约1.6倍。结论 陕西地区宫颈癌组织中HPV16 YY1位点突变的CTSE序列可激活启动子活性,YY1位点的突变与游离型HPV16 DNA 致宫颈癌有密切关系。 |
| 关 键 词： | 宫颈癌 游离型HPV16 细胞特异性增强子 YY1位点 启动子活性 |
| 收稿时间： | 2014-04-01 |
The Influence of Mutant Cell-type Specific Enhancer Fragment in Episomal HPV16 DNA on Activity of Viral Promoter in Cervical Carcinoma |
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| LI Ling,LIU Wen-kang,CHU Yong-lie,LIU Xiang,REN Jian-kang. The Influence of Mutant Cell-type Specific Enhancer Fragment in Episomal HPV16 DNA on Activity of Viral Promoter in Cervical Carcinoma[J]. Journal of Yanan University:Medical Science Edition, 2014, 12(2): 1-4 | |
| Authors: | LI Ling LIU Wen-kang CHU Yong-lie LIU Xiang REN Jian-kang |
| Affiliation: | LI Ling, LIU Wen-kang , CHU Yong-lie, LIU Xiang, REN Jian-kang(1 Department of Clinical laboratory, Shaanxi Provincial People＇s Hospital, Xi＇ an 710068, China; 2. Department of Immunology and Pathogenic Microbiology, Medical School of Xi＇ an Jiaotong University, Xi＇an 710061 ; China) |
| Abstract: | Objective To investigate the influence of mutant episomal HPV16 CTSE on promoter activity. Methods Recombinant CAT reporter plasmids containing wild HPV - 16 CTSE （ pCAT3 - Promoter - wCTSE） and mutant episomal HPV16 CTSE （ pCAT3 - Promoter - mCTSE） were constructed with pCAT3 - Promoter system. Functional analysis of the mutant CTSE and the wild - type CTSE was performed with CAT assay. Results 1. The recombinant plasmids showed a 1.5 - 2.5 fold increase in comparison to the CAT expressed of the pCAT3 - Promoter. 2. The re- combinant plasmids pCAT - 2 showed a 1.6 fold increase of the CAT expressed in comparison to pCAT - 1 contai- ning the prototype HPV - 16 CTSE. Conclusions Our results indicated the activity of promoter was up - regulated by YY1 site mutation in this CTSE. The mechanism of episomal HPV - associated carcinogenesis in Shaanxi is closely associated with YY1 site mutation in LCR. |
| Keywords: | cervical carcinoma episomal HPV16 cell- type specific enhancer mutation |
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