| 粪肠球菌脂磷壁酸对人牙周膜成纤维细胞表达Toll样受体2及白细胞介素-1β的影响 |
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| 引用本文： | Jin L,Luo X,Jiang Y,Xie X. 粪肠球菌脂磷壁酸对人牙周膜成纤维细胞表达Toll样受体2及白细胞介素-1β的影响[J]. 华西口腔医学杂志, 2012, 30(1): 93-96. DOI: 10.3969/j.issn.1000-1182.2012.01.022 |
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| 作者姓名： | Jin L Luo X Jiang Y Xie X |
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| 作者单位： | 中南大学湘雅医院口腔科, 长沙410008 |
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| 基金项目： | 湖南省科技厅基金资助项目(2010FJ3067) |
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| 摘 要： | 目的探讨体外培养的人牙周膜成纤维细胞（HPDLCs）在粪肠球菌脂磷壁酸（LTA）刺激下表达Toll样受体2（TLR2）及白细胞介素-1β（IL-1β）的情况。方法体外分离培养HPDLCs,采用流式细胞术检测0.1、1、10 μg·mL-1粪肠球菌LTA刺激24 h后HPDLCs表面TLR2表达的改变,酶联免疫吸附法检测12、24、48 h后IL-1β的分泌情况,并用相同质量浓度的大肠杆菌内毒素作为对照;用TLR2中和抗体预处理HPDLCs 1 h,观察1 μg·mL-1 LTA刺激24 h后其IL-1β的分泌量。结果LTA可致HPDLCs表面TLR2表达增加（P<0.05）;与对照组相比,粪肠球菌LTA可致HPDLCs分 泌IL-1β增加（P<0.05）,刺激12 h后可检测到IL-1β,48 h内呈上升趋势。TLR2中和抗体对粪肠球菌LTA诱导HPDLCs 分泌IL-1β无明显封闭作用。结论粪肠球菌LTA可引起HPDLCs表面TLR2表达及IL-1β分泌增加。
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| 关 键 词： | 粪肠球菌 脂磷壁酸 Toll样受体2 白细胞介素-1β 根尖周病 |
| 收稿时间： | 2012-02-25 |
| 修稿时间： | 2012-02-25 |
Study on the expression of Toll like receptor 2 and interleukin-1 beta induced by Enterococcus faecalis lipoteichoic acid on human periodontal ligament cells |
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| Jin Luyuan,Luo Xiaoliang,Jiang Yan,Xie Xiaoli. Study on the expression of Toll like receptor 2 and interleukin-1 beta induced by Enterococcus faecalis lipoteichoic acid on human periodontal ligament cells[J]. West China journal of stomatology, 2012, 30(1): 93-96. DOI: 10.3969/j.issn.1000-1182.2012.01.022 |
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| Authors: | Jin Luyuan Luo Xiaoliang Jiang Yan Xie Xiaoli |
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| Affiliation: | Dept. of Stomatology, Xiangya Hospital, Central-south University, Changsha 410008, China |
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| Abstract: | Objective To investigate the expression of Toll like receptor 2(TLR2) and interleukin-1β(IL-1β) of cultured human periodontal ligament cells(HPDLCs) activated by Enterococcus faecalis(E.faecalis) lipoteichoic acid(LTA).Methods HPDLCs that were obtained from the periodontal tissues of healthy humans were maintained in pro-per condition.Flow cytometry was used to detect the expression of TLR2 on normal HPDLCs and infectious HPDLCs which were incubated with 0.1,1,10 μg·mL-1 E.faecalis LTA for 24 h.IL-1β was detected by enzyme linked immunosorbent assay(ELISA) after incubating with LTA of the above concentration for 12,24 and 48 h or pretreated with TLR2 neutralizing antibody for 1 h and then co-cultured with 1 μg·mL-1 LTA for 24 h.Results E.faecalis LTA pro-moted the expression of TLR2 in normal HPDLCs.The difference had statistical significance(P<0.05).IL-1β secretion could be detected 12 h after stimulation with LTA and increasingly escalate within 48 h(P<0.05).TLR2 neutralizing antibody had no evident effect on IL-1β generation stimulating by E.faecalis LTA.Conclusion E.faecalis LTA can increase the expression of TLR2 and IL-1β in normal HPDLCs. |
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| Keywords: | Enterococcus faecalis lipoteichoic acid Toll like receptor 2 interleukin-1β periapical disease |
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