Multiplex PCR assay for identification of human diarrheagenic Escherichia coli |
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| Authors: | Toma Claudia Lu Yan Higa Naomi Nakasone Noboru Chinen Isabel Baschkier Ariela Rivas Marta Iwanaga Masaaki |
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| Affiliation: | Department of Bacteriology, Faculty of Medicine, University of the Ryukyus, Nishihara, Okinawa 903-0215, Japan. k950417@med.u-ryukyu.ac.jp |
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| Abstract: | A multiplex PCR assay for the identification of human diarrheagenic Escherichia coli was developed. The targets selected for each category were eae for enteropathogenic E. coli, stx for Shiga toxin-producing E. coli, elt and est for enterotoxigenic E. coli, ipaH for enteroinvasive E. coli, and aggR for enteroaggregative E. coli. This assay allowed the categorization of a diarrheagenic E. coli strain in a single reaction tube. |
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