| 江苏汉族人群p53第7内含子多态性与慢性萎缩性胃炎无相关性 |
| |
| 引用本文： | 王剑蓉,赖仁胜,张心海,谢玲. 江苏汉族人群p53第7内含子多态性与慢性萎缩性胃炎无相关性[J]. 世界华人消化杂志, 2007, 15(2): 171-175 |
| |
| 作者姓名： | 王剑蓉 赖仁胜 张心海 谢玲 |
| |
| 作者单位： | 南京中医药大学基础医学院,南京中医药大学附属医院病理科,国家中管局三级分子生物学实验室,江苏省南京市,210029;南京中医药大学附属医院病理科,国家中管局三级分子生物学实验室,江苏省南京市,210029;南京中医药大学,江苏省南京市,210029 |
| |
| 摘 要： | 目的:探讨江苏汉族人群p53第7内含子多态性与慢性萎缩性胃炎(CAG)之间的相关性.方法:提取80例CAG患者、76例健康体检人群基因组DNA,PCR特异性扩增p53基因第7,8外显子及其间的第7内含子,产物纯化后测序,将样品序列与标准序列进行比对,观察第7内舍子多态性.组间的基因型及等位基因频率比较采用χ~2检验.结果:CAG组p53基因第7内含子的第72位碱基和第92位碱基等位基因分别为T-G (32.5%)和C-T(67.5%),基因型表现为T-G/T-G (11.3%),C-T/T-G(42.5%)和C-T/C-T(46.3%)3种;在体检组中相应的等位基因T-G和C-T的频率是29.6%和70.4%,3种基因型频率分布分别为9.2%(T-G/T-G),40.8%(C-T/T-G)和50.0%(C-T/C-T).等位基因频率和基因型分布在CAG组和体检组差异无统计学意义(P>0.05),T-G等位基因携带者和C-T等位基因携带者患CAG的危险性差异无统计学意义(OR=1.14).结论:江苏汉族人群p53第7内含子多态性与CAG表遗传外显率无直接致病关系.
|
| 关 键 词： | 慢性萎缩性胃炎 p53基因 多态性 内含子 测序 |
| 修稿时间： | 2006-10-10 |
No association between p53 gene intron 7 polymorphism and chronic atrophic gastritis in Han Chinese of Jiangsu province |
| |
| Jian-Rong Wang,Ren-Sheng Lai,Xin-Hai Zhang,Ling Xie. No association between p53 gene intron 7 polymorphism and chronic atrophic gastritis in Han Chinese of Jiangsu province[J]. World Chinese Journal of Digestology, 2007, 15(2): 171-175 |
| |
| Authors: | Jian-Rong Wang Ren-Sheng Lai Xin-Hai Zhang Ling Xie |
| |
| Abstract: | AIM:To explore whether the polymorphism of p53 gene intron 7 is associated with the occurrence of chronic atrophic gastritis (CAG) in Han Chinese of Jiangsu province. METHODS:The genome DNA was extracted from tissues of 80 CAG patients and 76 healthy controls,and the exon 7 and 8 as well as the in- tron 7 within exon 7 and 8 of p53 gene were am- plified by polymerase chain reaction (PCR).The products were sequenced after purification.The polymorphism of p53 gene intron 7 was compar- atively analyzed with the standard sequences. The genotypes and allelic frequencies compared between the two groups using chi-square test. RESULTS:In CAG cases,the allelic frequen- cies of the 72~(th) base pair (bp) and the 92~(th) bp in p53 gene intron 7 were 32.5% (T-G) and 67.5% (C-T),and the frequencies of genotype T-G/T-G, C-T/T-G,and C-T/C-T were 11.3%,42.5%,and 46.3%,respectively.Meanwhile in the controls, the allelic frequencies of T-G and C-T were 29.6% and 70.4%,and the frequencies of geno- type T-G/T-G,C-T/T-G,and C-T/C-T were 9.2%,40.8%,and 50.0%,respectively.There were no significant differences in the allelic frequen- cies and genotypic distributions between the two groups (P>0.05).The risk of CAG occur- rence was not significantly different between the individuals with T-G and C-T allele (OR=1.14). CONCLUSION:There is no direct association between the polymorphism of p53 gene intron 7 and epigenetic penetrance of CAG in Han Chi- nese of Jiangsu province. |
| |
| Keywords: | Chronic atrophic gastritis p53 gene Polymorphism Intron Sequencing |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
| 点击此处可从《世界华人消化杂志》浏览原始摘要信息 |
|
点击此处可从《世界华人消化杂志》下载全文 |
|
