| RNA干扰CD36对大鼠肺泡巨噬细胞TGF-1活化影响 |
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| 引用本文： | 王欣, 柏建芸. RNA干扰CD36对大鼠肺泡巨噬细胞TGF-1活化影响[J]. 中国公共卫生, 2013, 29(3): 363-365. DOI: 10.11847/zgggws2013-29-03-17 |
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| 作者姓名： | 王欣 柏建芸 |
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| 作者单位： | 1.天津市疾病预防控制中心, 300011 |
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| 基金项目： | 国家自然科学基金(81102106) |
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| 摘 要： | 目的观察RNA干扰CD36基因表达对博莱霉素(BLM)诱导的大鼠肺泡巨噬细胞潜在转化生长因子-β1(L-TGF-β1)活化影响。方法实验设BLM组、Lv-shCD36(CD36 shRNA重组慢病毒)组、Lv-shCD36对照组,各组细胞均用BLM刺激,采用realtime-PCR和western blot检测细胞中CD36干扰效率;采用貂肺上皮细胞(CCL-64)增殖抑制实验检测培养上清中TGF-β1活性。结果Lv-shCD36组细胞CD36 mRNA表达均低于BLM组和Lv-shCD36对照组,与Lv-shCD36对照组比较,干扰效率可达83%;与BLM组、Lv-shCD36对照组比较,Lv-shCD36组细胞CD36蛋白表达均降低;Lv-shCD36组培养上清中活化转化生长因子-β1(TGF-β1)含量(91.82±36.79)pg/10 μL和TGF-β1活化率(19.80±7.98)%均低于BLM组和Lv-shCD36对照组(P<0.05)。结论通过RNA干扰CD36基因表达能够抑制BLM诱导的大鼠肺泡巨噬细胞分泌的L-TGF-β1的活化。
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| 关 键 词： | RNA干扰 CD36 肺泡巨噬细胞 潜在转化生长因子1 (L-TGF-1) |
| 收稿时间： | 2012-04-23 |
Influence of CD36 gene suppression induced by RNA interference on L-TGF-1 activation derived from macrophages in rats after bleomycin treatment |
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| WANG Xin, BAI Jian-yun. Influence of CD36 gene suppression induced by RNA interference on L-TGF-1 activation derived from macrophages in rats after bleomycin treatment[J]. Chinese Journal of Public Health, 2013, 29(3): 363-365. DOI: 10.11847/zgggws2013-29-03-17 |
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| Authors: | WANG Xin BAI Jian-yun |
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| Affiliation: | 1.Tianjin Municipal Center for Disease Control and Prevention, Tianjin 300011, China |
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| Abstract: | ObjectiveTo study the influence of CD36 gene suppression induced by RNA interference on transforming growth factor-β1(L-TGF-β1) activation derived from bleomycin-treated macrophages in rats.MethodsThe three groups including bleomycin(BLM) group,BLM+Lv-shCD36(a lentiviral vector expressing shRNA specific against rat CD36) group,and BLM+Lv-shCD36-NC(non-silence control lentivirus) group were set for the experiment.The cells in each group were incubated with bleomycin and then collected for detecting the interference effect with real-time PCR and western blot,and the supernatant was collected for detection of TGF-β1 by CCL-64 mink lung epithelial growth inhibition assay.ResultsThe expression of CD36 mRNA in the Lv-shCD36 group was significantly lower than in the BLM group and control group.Compared to the control group,the efficiency of interference was 83%.The expression of CD36 protein in the Lv-shCD36 group was also lower than in the BLM group and control group.The quantity (91.82±36.79 pg/10μL)and percent (19.80±7.98%)of active TGF-β1 in the Lv-shCD36 group were significantly lower than those in the BLM group and control group(P<0.05 for all).ConclusionSuppression of the CD36 expression by RNA interference could inhibit the activation of L-TGF-β1 derived from bleomycin-treated macrophages in rat. |
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| Keywords: | RNA interference CD36 alveolar macrophage latent transforming growth factor-1 |
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