| 两种实验动物感染艰难梭菌的特征比较 |
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| 引用本文： | 古文鹏,夏宜兰,李文革,王媛媛,张文竹,贾筱溪,伏晓庆,卢金星,吴媛. 两种实验动物感染艰难梭菌的特征比较[J]. 疾病监测, 2021, 36(4): 340-343. DOI: 10.3784/jbjc.202012110412 |
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| 作者姓名： | 古文鹏 夏宜兰 李文革 王媛媛 张文竹 贾筱溪 伏晓庆 卢金星 吴媛 |
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| 作者单位： | 云南省疾病预防控制中心,云南昆明650022;中国医学科学院医学生物学研究所,云南昆明650031;云南省第一人民医院,云南昆明650022;中国疾病预防控制中心传染病预防控制所,北京102206;云南省疾病预防控制中心,云南昆明650022 |
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| 基金项目： | 国家科技重大专项（No. 2018ZX10733402，No. 2017ZX10103010） |
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| 摘 要： | 目的 研究分析树鼩和BALB/c小鼠感染艰难梭菌的特征,比较和评估二者作为艰难梭菌动物感染模型的适用性.方法 分别以树鼩和BALB/c小鼠作为研究对象,抗生素组合喂养后灌胃艰难梭菌RT078(105 CFU芽孢/只),7 d内观察动物的感染特征和表现,对感染动物的肠道组织进行组织病理学检查和分析.结果 树鼩自灌胃第2天...
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| 关 键 词： | 艰难梭菌 动物模型 树鼩 BALB/c小鼠 组织病理学 |
| 收稿时间： | 2020-12-11 |
Comparison of characteristics of Clostridioides difficile infection in two kinds of laboratory animals |
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| Affiliation: | 1.Yunnan Provincial Center for Disease Control and Prevention, Kunming 650022, Yunnan, China2.Institute of Medical Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Kunming 650031, Yunnan, China3.The First People's Hospital of Yunnan Province, Kunming 650022, Yunnan, China4.National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China |
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| Abstract: | Objective To analyze the characteristics of Clostridioides difficile infection in tree shrews and BALB/c mice, and evaluate the applicability to use them as animal models for C. difficile infection. Methods Tree shrews and BALB/c mice were used respectively. The animals were fed with antibiotics cocktails and infected by oral gavage of 105 CFU spores of RT078 C. difficile strain. The infection characteristics and manifestations of the animals within 7 days were observed, and the histopathological examination of the intestinal tissues were conducted. Results The tcdA and tcdB genes were detected in all stool samples of tree shrews from the second day after gavage, while the positive rate of the virulence genes was 80% in BALB/c mice. Within 7 days of infection, No tree shrew and mouse died. The proportions of the animals with weight loss during the experiment was 8.80%±1.82% and 4.82%±2.19%, respectively. Comparison of the infection characteristics of the animals showed that 60% of the tree shrews had obvious diarrhea symptoms, but the BALB/c mice had no such symptom. The results of abdominal cavity anatomy showed obvious inflammation of intestinal mucosa and abdominal cavity in the tree shrews, but not in the ALB/c mice. The histopathological characteristics of the two kinds of animals were similar, manifested by the destruction of intestinal cells, the disorder of tissue structure and the infiltration of inflammatory cells. The lesions of ileum and colon occurred in tree shrews, while colon lesion occurred in BALB/c mice. Conclusion Both tree shrews and BALB/c mice can be used as animal models for C. difficile infection. The clinical manifestations and disease manifestations of tree shrews might be similar to human diseases. The selection of animal as C. difficile infection model mainly depends on the observation indicators and research purpose of different studies. |
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