| 妊娠早期外周血和蜕膜CD4~+CD25~+FoxP3~+/CD127~-T细胞检测及意义 |
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| 引用本文： | 蒋国静,邱丽华,许文君,林其德,祝捷. 妊娠早期外周血和蜕膜CD4~+CD25~+FoxP3~+/CD127~-T细胞检测及意义[J]. 上海交通大学学报(医学版), 2009, 29(3) |
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| 作者姓名： | 蒋国静 邱丽华 许文君 林其德 祝捷 |
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| 作者单位： | 上海交通大学,医学院仁济医院妇产科,上海,200001 |
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| 摘 要： | 目的 研究妊娠早期外周血和蜕膜组织中CD4+CD25+FoxP3+和CD4+CD25+CD127-T细胞的数量变化,并探讨相关性.方法 17例原因不明复发性流产(URSA)患者(URSA组)和20名正常健康早孕者(对照组)于人工流产时分别采集外周血和蜕膜组织,Ficoll密度梯度离心法分离单个核细胞悬液,分别加入CD4-PE-Cy5.5、CD25-FITC或CD25-APC和细胞表面标记CD127-PE或细胞内标记FoxP3-PE,流式细胞仪检测样本中CD4+CD25+FoxP3+和CD4+CD25+CD127-T细胞的百分比,并进行线性相关分析.结果 与对照组比较,URSA组蜕膜组织中CD4+CD25+ FoxP3+和CD4+CD25+ CD127-T细胞百分比明显降低(均P<0.05);两组间外周血CD4+CD25+ FoxP3+和CD4+CD25+ CD127-T细胞百分比比较差异无统计学意义.组内比较显示,两组外周血和蜕膜组织中CD4+CD25+FoxP3+与CD4+CD25+CD127-T细胞百分比比较,差异均无统计学意义(均P﹥0.05).相关分析显示,两组CD4+CD25+ FoxP3+与CD4+CD25+ CD127-T细胞的百分比在外周血(r=0.9858, r=0.6870)和蜕膜组织(r=0.6089, r=0.9583)均呈显著正相关(均P﹤0.05).结论 URSA的发生可能与蜕膜CD4+CD25+ 调节性T细胞数量减少有关,而外周血CD4+CD25+调节性T细胞可能不参与URSA的免疫发病机制.细胞表面标记CD127可能成为鉴定和分离CD4+CD25+调节性T细胞的一种新的特异性标记物.
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| 关 键 词： | CD4 CD25 T细胞 外周血 蜕膜组织 复发性流产 |
Proportions and role of CD4~+CD25~+FoxP3~+/CD127~-T cells in peripheral blood anddeciduas during early pregnancy |
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| JIANG Guo-jing,QIU Li-hua,XU Wen-jun,LIN Qi-de,ZHU Jie. Proportions and role of CD4~+CD25~+FoxP3~+/CD127~-T cells in peripheral blood anddeciduas during early pregnancy[J]. Journal of Shanghai Jiaotong University:Medical Science, 2009, 29(3) |
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| Authors: | JIANG Guo-jing QIU Li-hua XU Wen-jun LIN Qi-de ZHU Jie |
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| Affiliation: | Department of Obstetrics and Gynecology;Renji Hospital;School of Medicine;Shanghai Jiaotong University;Shanghai 200001;China |
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| Abstract: | Objective To detect the proportions and role of CD4+CD25+FoxP3+ and CD4+CD25+CD127-T cells in peripheral blood and deciduas during early pregnancy. Methods Peripheral blood and deciduas were obtained during induced abortion from 17 patients with unexplained recurrent spontaneous abortion(URSA) (URSA group) and 20 normal early pregnant women (control group),and mononuclear cells were isolated by Ficoll density gradient centrifugation. The proportions of CD4+CD25+FoxP3+ and CD4+CD25+CD127-T cells of both grou... |
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| Keywords: | FoxP3 CD127 |
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