| 过表达CHD5基因对肝癌细胞SMMC-7721生物学行为的影响 |
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| 引用本文： | 王继元,费建平,陆艺,姜保成. 过表达CHD5基因对肝癌细胞SMMC-7721生物学行为的影响[J]. 现代肿瘤医学, 2016, 0(9): 1357-1360. DOI: 10.3969/j.issn.1672-4992.2016.09.007 |
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| 作者姓名： | 王继元 费建平 陆艺 姜保成 |
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| 作者单位： | 上海交通大学医学院附属苏州九龙医院普外科，江苏 苏州 215000 |
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| 摘 要： | 目的:探讨过表达CHD5基因对人肝癌细胞SMMC-7721生物学行为的影响。方法:采用脂质体法将CHD5过表达质粒及对照质粒分别转染SMMC-7721细胞，Western blotting检测SMMC-7721细胞中CHD5蛋白的表达情况，CCK-8法检测SMMC-7721细胞生长增殖能力的变化，流式细胞仪检测细胞凋亡，Transwell实验检测细胞迁移和侵袭能力的变化。结果:转染CHD5过表达质粒后，SMMC-7721细胞CHD5蛋白表达水平显著增高（P<0.01），抑制了细胞增殖、迁移和侵袭（P<0.05），促进了细胞凋亡（P<0.05）。结论:过表达CHD5基因可抑制SMMC-7721细胞的增殖、迁移和侵袭并诱导其凋亡。
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| 关 键 词： | CHD5 肝癌细胞SMMC-7721 细胞增殖 细胞凋亡 迁移 侵袭 |
Effects of CHD5 on cells biological behavior in the hepatocellular carcinoma cell line SMMC -7721 |
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| Wang Jiyuan,Fei Jianping,Lu Yi,Jiang Baocheng. Effects of CHD5 on cells biological behavior in the hepatocellular carcinoma cell line SMMC -7721[J]. Journal of Modern Oncology, 2016, 0(9): 1357-1360. DOI: 10.3969/j.issn.1672-4992.2016.09.007 |
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| Authors: | Wang Jiyuan Fei Jianping Lu Yi Jiang Baocheng |
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| Affiliation: | Department of General Surgery，Suzhou Kowloon Hospital，Shanghai Jiaotong University School of Medicine，Jiangsu Suzhou 215000，China. |
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| Abstract: | Objective:To explore the effect of CHD5 on cells biological behavior in hepatocellular carcinoma cell line SMMC -7721.Methods:SMMC -7721 cells were transfected with pcDNA3.1(-)/CHD5 or negative control, Western blotting was performed to measure the expression of CHD5 protein,cell proliferation,apoptosis,migration and invasion was determined by CCK -8 assay,flow cytometry and transwell assay,respectively.Results:CHD5 was up-regulated after transfected with pcDNA3.1(-)/CHD5 in SMMC -7721 cells compared with NC(P <0.01),inhibi-ted cells proliferation(P <0.05),increased cells apoptosis(P <0.05),and the ability of invasion and migration was significantly increased(P <0.05).Conclusion:Overexpression of CHD5 in SMMC -7721 cells may suppress cells proliferation,invasion and migration,induce cells apoptosis. |
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| Keywords: | CHD5 hepatocellular carcinoma cell line SMMC -7721 proliferation apoptosis migration invasion |
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