| PBK/TOPK通过改变糖酵解关键酶表达水平增加结直肠癌细胞放射敏感性 |
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| 引用本文： | 郭佳星,王振堂,林 宇,王雨哲,额尔德木图,李 静,郁志龙,宝莹娜. PBK/TOPK通过改变糖酵解关键酶表达水平增加结直肠癌细胞放射敏感性[J]. 现代肿瘤医学, 2022, 0(7): 1167-1171. DOI: 10.3969/j.issn.1672-4992.2022.07.005 |
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| 作者姓名： | 郭佳星 王振堂 林 宇 王雨哲 额尔德木图 李 静 郁志龙 宝莹娜 |
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| 作者单位： | 内蒙古医科大学附属医院放疗科，内蒙古 呼和浩特 010050 |
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| 基金项目： | 内蒙古自治区自然科学基金（编号:2019MS08124）；内蒙古医科大学青年创新基金（编号:YKD2017QNCX074） |
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| 摘 要： | 目的:探讨在结直肠癌细胞中PDZ结合激酶(PDZ binding kinase,PBK)/T-淋巴因子激活的杀伤细胞来源的蛋白激酶(T-cell-originated protein kinase,TOPK)能否通过影响糖酵解关键酶表达改变放射敏感性.方法:通过慢病毒转染敲低LS174T细胞中的PBK/TOPK表达水平...
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| 关 键 词： | PBK/TOPK 糖酵解 葡萄糖转运蛋白1 己糖激酶2 放射敏感性 |
PBK/TOPK increases the radiosensitivity of colorectal cancer cells by changing the expression levels of key glycolytic enzymes |
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| GUO Jiaxing,WANG Zhentang,LIN Yu,WANG Yuzhe,Eerdemutu,LI Jing,YU Zhilong,BAO Yingna. PBK/TOPK increases the radiosensitivity of colorectal cancer cells by changing the expression levels of key glycolytic enzymes[J]. Journal of Modern Oncology, 2022, 0(7): 1167-1171. DOI: 10.3969/j.issn.1672-4992.2022.07.005 |
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| Authors: | GUO Jiaxing WANG Zhentang LIN Yu WANG Yuzhe Eerdemutu LI Jing YU Zhilong BAO Yingna |
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| Affiliation: | Department of Radiation Therapy,Affiliated Hospital of Inner Mongolia Medical University,Inner Mongolia Hohhot 010050,China. |
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| Abstract: | Objective:To explore whether PBK/TOPK has effect on the radiosensitivity by affecting the expression of key glycolytic enzymes in colorectal cancer cells.Methods:Lentivirus transfection was used to knocked down PBK/TOPK in LS174T cells.It was tested the radiosensitivity by cloning formation.The expression of PBK/TOPK,GLUT1 and HK2 were detected by Real time-PCR and Western blot.Results:The cloning formation of LS174T cells with knocking down PBK/TOPK was smaller than the control group.It showed that GLUT1 in LS174T cells with 8 Gy radiation alone decreased to the minimal expression at 48 h，but HK2 had an opposite expression that was highest expression at 48 h.Besides it was found that the expression of GLUT1 decreased in PBK/TOPK knocking down group and then showed an upward trend,the expression of HK2 showed a completely opposite change,with the highest expression at 48 h.GLUT1 showed an increasing trend within 48 h and reached a peak at 48h,then a decreasing trend appeared in the cell with PBK/TOPK knocking down combined with 8 Gy radiation,while the expression level of HK2 gradually decreased with time.Conclusion:Knockdown of PBK/TOPK can enhance the radiosensitivity of colorectal cancer cells.The mechanism may be related to the completely opposite changes in the expression of key glycolytic enzymes GLUT1 and HK2. |
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| Keywords: | PBK/TOPK glycolysis glucose transporter 1 hexokinase 2 radiotherapy sensitivity |
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