Immunodominant epitopes of the adhesin of Mycoplasma pneumoniae. |
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| Authors: | E Jacobs A Pilatschek B Gerstenecker K Oberle W Bredt |
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| Affiliation: | Institute for Medical Microbiology and Hygiene, University of Freiburg, Federal Republic of Germany. |
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| Abstract: | Overlapping octapeptides from the amino acid sequence of the adherence protein of Mycoplasma pneumoniae were synthesized and used as the antigen in an enzyme-linked immunosorbent assay with serum samples from M. pneumoniae-infected patients. Of a sequence of 338 amino acids positioned between leucine 801 and leucine 1139, only two regions with immunodominant continuous epitopes were detected. The immunoglobulin G and immunoglobulin M antibodies of child and adult patients reacted especially with the NH2-(810)-W-I-G-N-G-Y-R-Y peptide but also reacted with the NH2-(1124)-F-T-D-F-V-K-P-R peptide. |
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