| 靶向CD19和BCMA的CAR-NK92MI细胞的构建及抗肿瘤活性研究 |
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| 引用本文： | 余卓营,李晓瑞,尚凤琴,李申,王铁山,王建勋. 靶向CD19和BCMA的CAR-NK92MI细胞的构建及抗肿瘤活性研究[J]. 现代肿瘤医学, 2022, 0(5): 785-791. DOI: 10.3969/j.issn.1672-4992.2022.05.006 |
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| 作者姓名： | 余卓营 李晓瑞 尚凤琴 李申 王铁山 王建勋 |
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| 作者单位： | 北京中医药大学生命科学学院，北京 102488 |
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| 基金项目： | 王建勋高层次人才科研启动经费(编号:9011451310032)。 |
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| 摘 要： | 目的:探讨嵌合抗原受体(chimeric antigen receptor,CAR)结构对CAR-NK细胞特异性杀伤能力的增强情况.方法:利用逆转录病毒载体分别构建出稳定表达靶向B细胞成熟抗原(B cell maturation antigen,BCMA)与分化群抗原19(cluster of differentiat...
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| 关 键 词： | 嵌合抗原受体NK细胞 特异性杀伤 分化群抗原19 B细胞成熟抗原 |
Construction and antitumor activity of CAR-NK92MI cells targeting CD19 and BCMA |
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| YU Zhuoying,LI Xiaorui,SHANG Fengqin,LI Shen,WANG Tieshan,WANG Jianxun. Construction and antitumor activity of CAR-NK92MI cells targeting CD19 and BCMA[J]. Journal of Modern Oncology, 2022, 0(5): 785-791. DOI: 10.3969/j.issn.1672-4992.2022.05.006 |
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| Authors: | YU Zhuoying LI Xiaorui SHANG Fengqin LI Shen WANG Tieshan WANG Jianxun |
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| Affiliation: | School of Life Sciences，Beijing University of Chinese Medicine,Beijing 102488,China. |
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| Abstract: | Objective:To investigate the specific cytotoxicity of CAR-NK cells by using CAR structure.Methods:BCMA and CD19 CAR-NK92 MI cells were constructed by retroviral vector respectively.Flow cytometry was uesd to detected the CAR-positive rate of CAR-NK cells stained with MYC-PE antibody.The cytotoxic activity of these CAR-NK92 MI cells against CD19-expressing or BCMA-expressing tumor cells were detected by EX-luciferase assay, Incucyte Live-Cell Analysis Systems and flow cytometry.Results:ANTI-CD19-CAR-NK92 MI cells and ANTI-BCMA-CAR-NK92 MI cells with stable and high expression of CAR were successfully constructed.The results of these cytotoxicity assays showed that ANTI-CD19-CAR-NK92 MI cells exhibited better effector function against CD19-expressing tumor cells, and ANTI-BCMA-CAR-NK92 MI cells exhibited better effector function against BCMA-expressing tumor cells.Conclusion:CAR can significantly improved the specific cytotoxicity of CAR-NK cells. |
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| Keywords: | chimeric antigen receptor NK cells specific cytotoxicity CD19 BCMA |
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