Gamma scintigraphy and biodistribution of 99mTc‐cefotaxime sodium in preclinical models of bacterial infection and sterile inflammation |
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| Authors: | Derya Ilem‐Ozdemir Makbule Asikoglu Hayal Ozkilic Ferda Yilmaz Mine Hosgor‐Limoncu Semin Ayhan |
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| Affiliation: | 1. Faculty of Pharmacy, Department of Radiopharmacy, Ege University, Izmir, Turkey;2. Faculty of Medicine, Department of Nuclear Medicine, Ege University, Izmir, Turkey;3. Faculty of Pharmacy, Department of Pharmaceutical Microbiology, Ege University, Izmir, Turkey;4. Faculty of Medicine, Department of Pathology, Celal Bayar University, Manisa, Turkey |
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| Abstract: | 99mTc‐cefotaxime sodium (99mTc‐CEF) was developed and standardized under varying conditions of reducing and antioxidant agent concentration, pH, radioactivity dose, and reducing agent type. Labeling studies were performed by changing the selected parameters one by one, and optimum labeling conditions were determined. After observing the conditions for maximum labeling efficiency and stability, lyophilized freeze dry kits were prepared accordingly. Simple method for radiolabeling of CEF with 99mTc has been developed and standardized. Labeling efficiency of 99mTc‐CEF was assessed by both radio thin‐layer chromatography and radio high‐performance liquid chromatography and found higher than 90%. The labeled compound was found to be stable in saline and human serum up to 24 h. Two different freeze dry kits were developed and evaluated. Based on the data obtained from this study, both products were stable for 6 months with high labeling efficiency. The prepared cold kit was found sterile and pyrogen free. The bacterial infection and sterile inflammation imaging capacity of 99mTc‐CEF was evaluated. Based on the in vivo studies, 99mTc‐CEF has higher uptake in infected and inflamed thigh muscle than healthy thigh muscle. |
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| Keywords: | 99m‐technetium cefotaxime sodium radiolabeling radiolabeled antibiotic infection imaging inflammation imaging |
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