| NB4细胞和HL60细胞向粒细胞分化过程中细胞表面CD11b和CD18表达的比较 |
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| 引用本文： | 王长莹,支 蕾,姚 智,高 颖. NB4细胞和HL60细胞向粒细胞分化过程中细胞表面CD11b和CD18表达的比较[J]. 天津医科大学学报, 2016, 0(6): 465-468 |
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| 作者姓名： | 王长莹 支 蕾 姚 智 高 颖 |
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| 作者单位： | (天津医科大学基础医学院免疫学系,天津市细胞与分子免疫重点实验室,国家教育部免疫微环境与疾病重点实验室,天津 300070) |
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| 摘 要： | 目的:比较髓系白血病细胞株NB4细胞和HL60细胞向粒细胞分化过程中细胞表面抗原CD11b和CD18的表达。方法:用全反式维甲酸(ATRA)诱导NB4细胞和HL60细胞向粒细胞定向分化，用MGG染色法进行初步鉴定，用FACS检测并比较两株细胞在分化前后细胞表面CD11b和CD18的变化，用Western blot检测两株细胞分化前后CD18的蛋白表达。结果:与分化前相比，两株细胞分化后细胞表面CD11b和CD18的表达均明显升高，并且这样的变化在NB4细胞中较HL60细胞更加显著。结论:分化NB4细胞可能较分化HL60细胞是更方便的粒细胞模型。
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| 关 键 词： | NB4细胞 HL60细胞 CD11b CD18 |
Comparison of cell surface expressions of CD11b and CD18 in NB4 cells and HL60 cells during myeloid differentiation |
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| WANG Chang-ying,ZHI Lei,YAO Zhi,GAO Ying. Comparison of cell surface expressions of CD11b and CD18 in NB4 cells and HL60 cells during myeloid differentiation[J]. Journal of Tianjin Medical University, 2016, 0(6): 465-468 |
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| Authors: | WANG Chang-ying ZHI Lei YAO Zhi GAO Ying |
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| Affiliation: | (Department of Immunology, School of Basic Medical Sciences, Tianjin Medical University, Tianjin Key Laboratory of Cellular and Molecular Immunology, Key Laboratory of Educational Ministry, Tianjin 300070, China ) |
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| Abstract: | Objective: To compare the expressions of cell surface antigen CD11b and CD18 in myeloid leukemia cell lines NB4 cells and HL60 cells in myeloid differentiation. Methods: NB4 cells and HL60 cells were induced to differentiate to mature granulocytes by All-trans retinoic acid (ATRA), and cell morphology was tested by MGG staining. Cell surface expressions of CD11b and CD18 of these two cell lines during differentiation were tested by flowcytometry. Expression of CD18 protein of these two cell lines in differentiation was tested by Western blot. Results :Compared with the undifferentiated cells, cell surface expressions of CD11b and CD18 were both upregulated in differentiated NB4 cells and differentiated HL60 cells. And these changes were more significant in differentiated NB4 cells than in differentiated HL60 cells. Conclusion: It might be more convenient to study myeloid cells in differentiated NB4 cells than in differentiated HL60 cells |
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| Keywords: | NB4 cells HL60 cells CD11b CD18 |
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