| PRDM1 基因对非霍奇金淋巴瘤细胞增殖能力的影响 |
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| 引用本文： | 徐理华, 李玺, 谭获. PRDM1 基因对非霍奇金淋巴瘤细胞增殖能力的影响[J]. 分子影像学杂志, 2016, 39(3): 306-308. doi: 10.3969/j.issn.1674-4500.2016.03.31 |
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| 作者姓名： | 徐理华 李玺 谭获 |
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| 作者单位： | 1.广州医科大学附属第一医院血液科，广东 广州 510230;;2.中山大学附属第三医院甲乳外科，广东 广州 510630 |
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| 摘 要： | 目的 观察PRDM1对非霍奇金淋巴瘤细胞SUDHL-4增殖能力的影响。方法 通过质粒转染的方法在低表达PRDM1的SUDHL-4细胞中过表达PRDM1基因，通过MTT实验、细胞周期分析检测SUDHL-4生物学行为的变化。结果 过表达PRDM1的SUDHL-4细胞的生长速度明显下降，细胞G0/G1比例、S期比例下降；G2/M期比例升高,细胞阻滞于G2/M期，差异均有统计学意义（P<0.05）。结论 PRDM1基因表达缺失可能在非霍奇金淋巴瘤的发生发展中起重要作用，可能成为判断非霍奇金淋巴瘤预后的分子标志物及临床治疗的靶点。
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| 关 键 词： | 非霍奇金淋巴瘤 PRDM1 SUDHL-4细胞 |
| 收稿时间： | 2016-04-15 |
Effects of PRDM1gene expression on the reproductive ability of non-Hodgkin lymphoma cells |
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| Lihua XU, Xi LI, Huo TAN. Effects of PRDM1gene expression on the reproductive ability of non-Hodgkin lymphoma cells[J]. Journal of Molecular Imaging, 2016, 39(3): 306-308. doi: 10.3969/j.issn.1674-4500.2016.03.31 |
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| Authors: | Lihua XU Xi LI Huo TAN |
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| Affiliation: | 1. Department of Hematology, the First Affiliated Hospital of Guangzhou Medical University, Guangzhou 510230, China;;2. Department of thyroid surgery, the Third Affiliated Hospital of Zhongshan University, Guangzhou 510630, China |
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| Abstract: | Objective To explore the effects of PRDM1gene expression on the reproductive ability of Non-Hodgkin Lymphoma cells SUDHL-4．Methods Non-Hodgkin Lymphoma cells SUDHL-4 was chosed to show the biology behavior changes after overexpressing PRDM1 gene, through the MTT test, and cell cycle analysis. Results After overexpressing PRDM1 gene, the proliferation of SUDHL-4 cell were significantly decreased, and cell cycle arrested at G2/M (P<0.05). Conclusion The loss of PRDM1 gene may be related to the occurrence and development of lymphoma, and it may become the molecular diagnostics and new therapy target of lymphoma. |
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| Keywords: | non-Hodgkin lymphoma PRDM1 SUDHL-4 cell |
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