| 慢性丙型肝炎患者外周血滤泡辅助性T淋巴细胞表面PD-1受体表达及意义研究* |
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| 引用本文： | 吴静,东保吉,吴超. 慢性丙型肝炎患者外周血滤泡辅助性T淋巴细胞表面PD-1受体表达及意义研究*[J]. 实用肝脏病杂志, 2019, 22(2): 192-195. DOI: 10.3969/j.issn.1672-5069.2019.02.010 |
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| 作者姓名： | 吴静 东保吉 吴超 |
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| 作者单位： | 844000 新疆维吾尔自治区喀什市 喀什地区第一人民医院感染病科（吴静,吴超）; 新疆医科大学第六附属医院感染办公室（东保吉 |
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| 基金项目： | *新疆维吾尔自治区自然科学基金资助项目（编号：54200152） |
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| 摘 要： | 目的 探讨不同血清病毒载量的慢性丙型肝炎（CHC）患者外周血滤泡辅助性T淋巴细胞（Tfh）表面程序性死亡受体-1（PD-1）表达情况。方法 根据血清HCV RNA水平不同,将180例CHC患者分为低病毒载量组76例,3 lg copies/ml≤血清HCV RNA<6 lg copies/ml和高病毒载量组104例,血清HCV RNA≥6 lg copies/ml。比较两组外周血Tfh细胞百分比、Tfh细胞表面PD-1阳性率、外周血T、B淋巴细胞亚群、外周血CD4+T淋巴细胞和CD8+T淋巴细胞表面PD-1表达和血清白细胞介素21（IL-21）水平的差异。结果 低病毒载量组和高病毒载量组血清HCV RNA水平分别为（4.5±1.2）lg copies/ml和（6.4±0.7）lg copies/ml,Tfh细胞表面PD-1阳性百分比分别为（26.2±2.2）%和（37.2±1.1）%,Tfh细胞百分比分别为（7.9±0.7）%和（5.1±0.4）%,血清IL-21水平分别为（46.8±1.3） ng/L和（21.7±1.1） ng/L,差异均有统计学意义（P<0.01）;低病毒载量组和高病毒载量组外周血CD4+T细胞百分比分别为（51.1±4.6）%和（37.6±4.4）%,CD8+T细胞百分比分别为（24.0±3.1）%和（31.7±3.9）%, CD4+T/CD8+T细胞比值分别为（3.3±0.2）和（2.3±0.1）,CD19+B细胞百分比分别为（16.7±3.9）%和（11.8±3.2）%,差异具有统计学意义（P<0.01）;低病毒载量组和高病毒载量组外周血CD4+T细胞表面PD-1阳性率分别为（10.1±2.3）%和（2.4±0.6）%],CD8+T细胞表面PD-1阳性率分别为（6.3±2.2）%和（1.0±0.3）%,差异也具有统计学意义（P<0.01）。结论 不同血清病毒载量的CHC患者外周血Tfh和T淋巴细胞亚群以及血清白细胞介素21水平存在显著差异,进一步探讨它们对病情、抗病毒治疗应答和预后的关系,将具有十分重要的临床意义。
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| 关 键 词： | 慢性丙型肝炎 病毒载量 滤泡辅助性T淋巴细胞 程序性死亡受体1 白细胞介素21 |
| 收稿时间： | 2018-06-01 |
Comparison of PD-1 receptor on peripheral blood follicular helper T lymphocyte surface in chronic hepatitis C patients with low and high serum viral loads |
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| Wu Jing,Dong Baoji,Wu Chao. Comparison of PD-1 receptor on peripheral blood follicular helper T lymphocyte surface in chronic hepatitis C patients with low and high serum viral loads[J]. Journal of Clinical Hepatology, 2019, 22(2): 192-195. DOI: 10.3969/j.issn.1672-5069.2019.02.010 |
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| Authors: | Wu Jing Dong Baoji Wu Chao |
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| Affiliation: | Department of Infectious Diseases,First People's Hospital,Kashi 844000,Xinjiang Uyghur Autonomous Region,China |
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| Abstract: | Objective To compare the difference of programmed cell death receptor-1 （PD-1） receptor on peripheral blood follicular helper T lymphocyte （Tfh） surface in chronic hepatitis C （CHC） patients with low and high serum viral loads. Methods 180 patients with CHC were divided into two group,and 76 had serum HCV RNA level of less than 6 lg copies/ml and 104 had serum HCV RNA level of greater than 6 lg copies/ml. The percentage of PD-1 on peripheral blood Tfh cell surface,percentage of Tfh,peripheral blood T and B lymphocyte subsets and serum interleukin 21 （IL-21） level were detected. Results In patients with low and high viral load groups,serum HCV RNA levels were （4.5±1.2） lg copies/ml and （6.4±0.7） lg copies/ml,the percentages of PD-1 on Tfh cell surface were （26.2±2.2）% and （37.2±1.1）%,the percentages of Tfh cells were （7.9±0.7）% and （5.1±0.4）%,and serum IL-21 levels were（46.8±1.3） ng/l and（21.7±1.1） ng/l,all significantly different between the two groups （P<0.05）;the percentages of CD4+T lymphocyte were （51.1±4.6）% and （37.6±4.4）%], the percentages of CD8+T cell were （24.0±3.1）% and （31.7±3.9）%],the ratios of CD4+T/CD8+T cells were （3.3±0.2） and （2.3±0.1）],the percentage of CD19+B cell were（16.7±3.9）% and（11.8±3.2）%,all significantly different between the two groups（P<0.05）;the percentages of PD-1 on CD4+T lymphocyte surface were（10.1±2.3）% and （2.4±0.6）%],and the percentages of PD-1 on CD8+T cell surface were （6.3±2.2）% and（1.0±0.3）% （both P<0.01）. Conclusion There were significant differences between patients with low and high serum HCV RNA loads as respect to their peripheral blood Tfh and PD-1 expression,which might be important in evaluating the response to antiviral therapy and prognosis. |
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| Keywords: | Chronic hepatitis C Viral loads Follicle helper T lymphocytes Programmed cell death receptor-1 Interleukin 21 |
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